1.0 - install packages, load libraries

Here we source the bit of code that performs this operation. essentially, this one-line command runs an entire R script file. This can be handy when you load the same libraries in different steps of your analysis

source("./code/install_packages_load_libraries.R")

2.0 - load and check data

Here we will load data from 3 species in the Brassicacea family experiment: Arabdopsis thaliana, Brassica oleraceae and Isatis tinctoria. This data has been heavily filtered to reduce complexity and size.

You will see that the data is stored as a list of 3 phyloseq objects. we can acess this in different ways

# load object
load("./data/ps_rarefied.Rdata")

#check ps object
ps_rarefied # full ps object
## phyloseq-class experiment-level object
## otu_table()   OTU Table:         [ 1000 taxa and 143 samples ]
## sample_data() Sample Data:       [ 143 samples by 44 sample variables ]
## tax_table()   Taxonomy Table:    [ 1000 taxa by 8 taxonomic ranks ]
## refseq()      DNAStringSet:      [ 1000 reference sequences ]
otu_table(ps_rarefied)[1:5,1:5] # 5 first rows across 5 first columns
## OTU Table:          [5 taxa and 5 samples]
##                      taxa are rows
##         49_16S 50_16S 51_16S 52_16S 53_16S
## bASV_5      90    299    109     87    231
## bASV_7      25    156    154    446     58
## bASV_9     157    191    264    108    145
## bASV_11    337    248    193    216    100
## bASV_12    201    437    272     71    154
tax_table(ps_rarefied)[1:5,] # 5 first rows across all columns
## Taxonomy Table:     [5 taxa by 8 taxonomic ranks]:
##         Kingdom       Phylum                Class                   
## bASV_5  "k__Bacteria" "p__Proteobacteria"   "c__Gammaproteobacteria"
## bASV_7  "k__Bacteria" "p__Proteobacteria"   "c__Gammaproteobacteria"
## bASV_9  "k__Bacteria" "p__Bacteroidota"     "c__Bacteroidia"        
## bASV_11 "k__Bacteria" "p__Proteobacteria"   "c__Alphaproteobacteria"
## bASV_12 "k__Bacteria" "p__Actinobacteriota" "c__Actinobacteria"     
##         Order                 Family                 Genus             Species
## bASV_5  "o__Burkholderiales"  "f__Comamonadaceae"    "g__Acidovorax"   "s__"  
## bASV_7  "o__Pseudomonadales"  "f__Pseudomonadaceae"  "g__Pseudomonas"  "s__"  
## bASV_9  "o__Chitinophagales"  "f__Chitinophagaceae"  "g__Niastella"    "s__"  
## bASV_11 "o__Sphingomonadales" "f__Sphingomonadaceae" "g__Sphingomonas" "s__"  
## bASV_12 "o__Streptomycetales" "f__Streptomycetaceae" "g__Streptomyces" "s__"  
##         ASV_id   
## bASV_5  "bASV_5" 
## bASV_7  "bASV_7" 
## bASV_9  "bASV_9" 
## bASV_11 "bASV_11"
## bASV_12 "bASV_12"
ps_rarefied@sam_data [1:5,1:5]# metadata
##        Target Harvest_ID Block_row_column Sp_speed  Stress
## 49_16S    16S         49            1.2.4       M1 Control
## 50_16S    16S         50            2.3.1       M1 Control
## 51_16S    16S         51            3.2.9       M1 Control
## 52_16S    16S         52            4.1.6       M1 Control
## 53_16S    16S         53           5.6.14       M1 Control

3.0 - create a single taxmap object for a single heat tree

For a simple start, let’s create individual heat trees from an individual plyloseq object

# make a copy of you rarefied ps object
ps_rarefied_filt<-ps_rarefied

#remove unecessary taxonomic info (dada2id, "s__" and "ASV_id") by updating the tax table with a subset of the tax table
tax_table(ps_rarefied_filt)<-tax_table(ps_rarefied_filt)[,1:6]


# let's remove the "r__"ranks from the taxonomy, they can be useful but will pollute our plot
tax_table(ps_rarefied_filt)[, colnames(tax_table(ps_rarefied_filt))] <- gsub(pattern = "[a-z]__", # regular expression 
                                                                   x = tax_table(ps_rarefied_filt)[, colnames(tax_table(ps_rarefied_filt))], # "df"
                                                                   replacement = "") # replacement for pattern
# transform from phyloseq to taxmap object
taxmap_obj<-parse_phyloseq(ps_rarefied_filt)

3.1 - EXERCISE check taxmap object you just created

taxmap_obj is a object from the Taxmap class, but can be navigated as a nested list. explore taxmap_obj with “$” and tab-completion. can you find the representative sequences in the data layer?

# the taxmap object is a data container, just like phyloseq
taxmap_obj

# this object has much more than what you can see on one print, just like much other R output you generate. 
taxmap_obj$data$tax_data # otu tables...
taxmap_obj$taxa # taxonomy names, rnks and codes

3.2 - create the heat tree visualization

Now we can create a heat tree, with colors indicating the number of sequences in each taxa.

The first tree is actually quite busy - depending on your goal, you might want to further filter the input ASVs to a smaller subset like all differentially abudnant ASVs or features defined as core microbiome communities

# create a heat tree!
set.seed(1) # set a seed so the layout can be exactly the same every time
heat_tree(.input = taxmap_obj, # your taxmap object from the metacoder package
          node_size = n_obs, # n_obs is a function that calculates the number of OTUs per taxon 
          node_color = n_obs, # this function from metacoder is called with non-standard evaluation (no arguments)
          node_label = taxon_names, # labels for the taxons
          layout = "davidson-harel", # The primary layout algorithm
          initial_layout = "reingold-tilford", # The layout algorithm that initializes node locations
          output_file = "./results/heat_tree_test.pdf") # A PDF file you will export this plot to

Can you well which is the most diverse phylum? which are the 3 most diverse families?

QUESTION BREAK

3.3 - EXERCISE check arguments

check what’s produced with the n_obs argument/function used in the tree above and also check the taxa names

#n_obs is a function that takesyour taxmap object as input, and return the number of sequences in each taxa
n_obs(taxmap_obj)

#in metacoder, ab, ac, ad, etc refer each to a different taxonomy
taxmap_obj$taxa

3.4 - Remove some taxa the heat tree visualization

It might be too dense/complex to look, so let’s remove taxa names that can be unfamiliar like “phylum NB1-J”

# create a heat cleaner tree!
taxmap_obj %>%
  metacoder::filter_taxa(grepl(pattern = "^[a-zA-Z]+$", taxon_names)) %>% # remove "odd" taxa
  heat_tree(node_label = taxon_names,
            node_size = n_obs,
            node_color = n_obs,
            layout = "davidson-harel",
            initial_layout = "reingold-tilford",
            output_file = "./results/heat_tree_test_cleaner.pdf")

3.5 - modify the tree with available options

There are many options to customize these trees, we will only explore a few of them

3.5.1 - truncate at order level

This tree stops at Order level

# create a heat cleaner tree!
taxmap_obj %>%
  metacoder::filter_taxa(grepl(pattern = "^[a-zA-Z]+$", taxon_names)) %>% # remove "odd" taxa
  metacoder::filter_taxa(taxon_ranks == "Order", supertaxa = TRUE) %>% # subset to the Order rank
  heat_tree(node_label = taxon_names,
            node_size = n_obs,
            node_color = n_obs,
            layout = "davidson-harel",
            initial_layout = "reingold-tilford",
            output_file = "./results/heat_tree_order.pdf")

3.5.2 - make individual roots for specific taxonomic groups

This tree has a separated root at different taxonomic levels, and custom colors

# create a heat cleaner tree!
taxmap_obj %>%
  metacoder::filter_taxa(grepl(pattern = "^[a-zA-Z]+$", taxon_names)) %>% # remove "odd" taxa
  metacoder::filter_taxa(taxon_names %in% c("Rhizobiales", "Actinobacteria", "Xanthomonadaceae"),
              subtaxa = TRUE, 
              supertaxa = FALSE) %>% 
  heat_tree(node_label = taxon_names,
            node_size = n_obs,
            node_color = n_obs,
            node_color_range = c("blue", "yellow", "pink", "red"),
            layout = "davidson-harel",
            initial_layout = "reingold-tilford",
            output_file = "./results/heat_tree_sep_roots.pdf")

3.6 - Compare 2 groups of samples

lets now compare ASV abundances between two groups of samples, such as plant species in brassicaceae lineage I and II (Sp_Lineage_Walden).

3.6.1 - calculate taxon abundance

“tax_abund” is added as a data layer of the taxmap object. it defines the number of sequences on every single taxon - all phyla, all classes, orders… this is different from counts of every single ASV! this data layer is needed on the next step.

#get abundance per taxon
taxmap_obj$data$tax_abund<-calc_taxon_abund(obj = taxmap_obj, # the taxmap object
                                            data = "otu_table", # layer of data table to use
                                            cols = taxmap_obj$data$sample_data$sample_id) #sample names

#check the new data layer you created. note that "ab" refers to "k__Bacteria",  and "aac" to "p__Proteobacteria".  
taxmap_obj$data$tax_abund
## # A tibble: 457 x 144
##    taxon_id `49_16S` `50_16S` `51_16S` `52_16S` `53_16S` 54_16~1 55_16~2 56_16~3
##    <chr>       <dbl>    <dbl>    <dbl>    <dbl>    <dbl>   <dbl>   <dbl>   <dbl>
##  1 ab          10000    10000    10000    10000    10000   10000   10000   10000
##  2 ac           7137     7430     6677     7995     7774    7459    7183    7574
##  3 ad           1188     1012     1351      739      769     951    1242     998
##  4 ae            739     1152     1129      295      535     938    1085     829
##  5 af            355      101      349      363      267     186     135     165
##  6 ag            191       82      189      309      286     208     134     147
##  7 ah             42       65       80       22       40      47      28      81
##  8 ai             23       24       43       38       40      21      13      27
##  9 aj             10        7       18       31       12       5       6       0
## 10 ak             16       18       21       13       25      19      27      17
## # ... with 447 more rows, 135 more variables: `57_16S` <dbl>, `58_16S` <dbl>,
## #   `59_16S` <dbl>, `60_16S` <dbl>, `61_16S` <dbl>, `62_16S` <dbl>,
## #   `63_16S` <dbl>, `64_16S` <dbl>, `65_16S` <dbl>, `66_16S` <dbl>,
## #   `67_16S` <dbl>, `68_16S` <dbl>, `69_16S` <dbl>, `70_16S` <dbl>,
## #   `71_16S` <dbl>, `72_16S` <dbl>, `73_16S` <dbl>, `74_16S` <dbl>,
## #   `75_16S` <dbl>, `76_16S` <dbl>, `77_16S` <dbl>, `78_16S` <dbl>,
## #   `79_16S` <dbl>, `80_16S` <dbl>, `81_16S` <dbl>, `82_16S` <dbl>, ...
#quickly compare those values to the OTU table to see the differnce:
taxmap_obj$data$otu_table
## # A tibble: 1,000 x 145
##    taxon_id otu_id  `49_16S` `50_16S` `51_16S` `52_16S` `53_16S` 54_16~1 55_16~2
##    <chr>    <chr>      <dbl>    <dbl>    <dbl>    <dbl>    <dbl>   <dbl>   <dbl>
##  1 jq       bASV_5        90      299      109       87      231      40      78
##  2 jr       bASV_7        25      156      154      446       58     191     376
##  3 js       bASV_9       157      191      264      108      145     128     245
##  4 jt       bASV_11      337      248      193      216      100     499     403
##  5 ju       bASV_12      201      437      272       71      154     240     362
##  6 jv       bASV_13      226      178       89      162      115     255     230
##  7 jw       bASV_14      374      159      301      230      321     167     229
##  8 fi       bASV_15      157       49       72      135       26     199      92
##  9 jy       bASV_16       62      373      173       96      144     255     201
## 10 fj       bASV_18      317      160      161      143      191     165     182
## # ... with 990 more rows, 136 more variables: `56_16S` <dbl>, `57_16S` <dbl>,
## #   `58_16S` <dbl>, `59_16S` <dbl>, `60_16S` <dbl>, `61_16S` <dbl>,
## #   `62_16S` <dbl>, `63_16S` <dbl>, `64_16S` <dbl>, `65_16S` <dbl>,
## #   `66_16S` <dbl>, `67_16S` <dbl>, `68_16S` <dbl>, `69_16S` <dbl>,
## #   `70_16S` <dbl>, `71_16S` <dbl>, `72_16S` <dbl>, `73_16S` <dbl>,
## #   `74_16S` <dbl>, `75_16S` <dbl>, `76_16S` <dbl>, `77_16S` <dbl>,
## #   `78_16S` <dbl>, `79_16S` <dbl>, `80_16S` <dbl>, `81_16S` <dbl>, ...

3.6.2 - calculate taxon prevalence/occumpancy

here we define taxa “occurrence” by determining in how many samples of each group they appear

#get occurrence of per lineage
taxmap_obj$data$tax_occ_2lineages <- calc_n_samples(obj = taxmap_obj, 
                                                    data = "tax_abund", 
                                                    cols = taxmap_obj$data$sample_data$sample_id,
                                                    groups = taxmap_obj$data$sample_data$Sp_Lineage_Walden) # What category each sample is



#check the object. it shows how many times a taxon is represented in lineage I or II
taxmap_obj$data$tax_occ_2lineages
## # A tibble: 457 x 3
##    taxon_id lineage_II lineage_I
##    <chr>         <int>     <int>
##  1 ab               95        48
##  2 ac               95        48
##  3 ad               95        48
##  4 ae               95        48
##  5 af               95        48
##  6 ag               95        48
##  7 ah               95        48
##  8 ai               95        48
##  9 aj               94        40
## 10 ak               95        48
## # ... with 447 more rows

3.6.3 - calculate log fold changes between two groups

Here we see log fold changes

# this will calculate log2 median ratios and p values for a wilcoxcon test within taxas in this lineage
taxmap_obj$data$diff_table_2lineages <- compare_groups(obj = taxmap_obj,
                                                      data = "tax_abund",
                                                      cols = taxmap_obj$data$sample_data$sample_id, 
                                                      groups = taxmap_obj$data$sample_data$Sp_Lineage_Walden) 

#check the object.
taxmap_obj$data$diff_table_2lineages
## # A tibble: 457 x 7
##    taxon_id treatment_1 treatment_2 log2_median_ratio media~1 mean_d~2 wilcox_~3
##    <chr>    <chr>       <chr>                   <dbl>   <dbl>    <dbl>     <dbl>
##  1 ab       lineage_II  lineage_I              0          0    0       NaN      
##  2 ac       lineage_II  lineage_I              0.126    624.   5.56e+2   1.34e-7
##  3 ad       lineage_II  lineage_I              0.0991    68   -4.57e+0   4.77e-1
##  4 ae       lineage_II  lineage_I             -0.795   -394.  -3.58e+2   5.75e-7
##  5 af       lineage_II  lineage_I              0.0316     5.5 -1.54e-2   9.76e-1
##  6 ag       lineage_II  lineage_I             -0.448    -65.5 -6.67e+1   1.77e-5
##  7 ah       lineage_II  lineage_I             -0.257    -11.5 -1.05e+1   1.93e-1
##  8 ai       lineage_II  lineage_I             -0.665    -20.5 -2.11e+1   1.10e-6
##  9 aj       lineage_II  lineage_I             -0.115     -1   -7.21e-1   7.78e-1
## 10 ak       lineage_II  lineage_I             -0.445     -6.5 -6.32e+0   1.23e-3
## # ... with 447 more rows, and abbreviated variable names 1: median_diff,
## #   2: mean_diff, 3: wilcox_p_value

3.6.4 - plot heat tree showing log-fold differences

tan indicates lineage II, cyan indicates lineage I

taxmap_obj %>%
  metacoder::filter_taxa(grepl(pattern = "^[a-zA-Z]+$", taxon_names)) %>% # remove "odd" taxa
  heat_tree(node_label = taxon_names,
            node_size = n_obs,
            node_color = log2_median_ratio,
            node_color_interval = c(-3, 3), # The range of `log2_median_ratio` to display
            node_color_range = c("cyan", "gray", "tan"), # The color palette used
            layout = "davidson-harel",
            node_color_axis_label = "Log2 ratio, cyan for Lineage I",
            initial_layout = "reingold-tilford",
            output_file = "./results/heat_tree_diff_lineages.pdf")

3.6.5 - supress non-significant differences and plot log changes again

this plot indicates log fold changes, but includes p values > 0.05. let’s run any non-significat differences into a median log ratio of zero, shading them as grey in the plot

# set differential log ratio to 0 based on p values below 0.05
taxmap_obj$data$diff_table_2lineages$log2_median_ratio[taxmap_obj$data$diff_table_2lineages$wilcox_p_value > 0.05] <- 0

# plot the heatmap
taxmap_obj %>%
  metacoder::filter_taxa(grepl(pattern = "^[a-zA-Z]+$", taxon_names)) %>% # remove "odd" taxa
  heat_tree(node_label = taxon_names,
            node_size = n_obs,
            node_color = log2_median_ratio,
            node_color_interval = c(-3, 3), # The range of `log2_median_ratio` to display
            node_color_range = c("cyan", "gray", "tan"), # The color palette used
            layout = "davidson-harel",
            node_color_axis_label = "Log2 ratio, cyan for Lineage I",
            initial_layout = "reingold-tilford",
            output_file = "./results/heat_tree_diff_lineages_pcut.pdf")

From this figure, we could see a larger presence of low-diversity phyla in lineage I (cyan). lineage II has a clear increase in Sphingomonadaceae. both lineages seem to be colonized by several, but different, bacteroidia and actinobacteria. Family Comamonadaceae and Chitinophagaceae seem to be the most diverse

QUESTION BREAK

3.7 - Compare 3 or more groups of samples

If you have more than 2 groups, like we have 3 different stresses, you can plot a matrix of heat trees with a dedicated function from the same package. before we do that, let’s first calculate taxon abundance, prevalence, and log-fold changes like we did in chunks 3.5.1 to 3.5.3

3.7.1 - calculate abudance, prevalence and log-fold in 3 treatment groups

#abundance per taxon was already calculated in chunk 3.5.1, now we jsut overwrite
taxmap_obj$data$tax_abund<-calc_taxon_abund(obj = taxmap_obj, 
                                            data = "otu_table", 
                                            cols = taxmap_obj$data$sample_data$sample_id) 

#get occurrence of ASVs per treatment
taxmap_obj$data$tax_occ_3treatments <- calc_n_samples(obj = taxmap_obj, 
                                                      data = "tax_abund", 
                                                      cols = taxmap_obj$data$sample_data$sample_id,
                                                      groups = taxmap_obj$data$sample_data$Stress) # now refer to Stress, that has 3 groups

# calculate log2 median ratios and p values for a wilcoxcon test within taxas in this stress treatment groups
taxmap_obj$data$diff_table_3treatments <- compare_groups(obj = taxmap_obj,
                                                        data = "tax_abund",
                                                        cols = taxmap_obj$data$sample_data$sample_id, 
                                                        groups = taxmap_obj$data$sample_data$Stress) 

# set differential log ratio to 0 based on adjusted p values
taxmap_obj$data$diff_table_3treatments$log2_median_ratio[taxmap_obj$data$diff_table_3treatments$wilcox_p_value > 0.05] <- 0

# because of potential ambiguity in non-standard evaluation, we should change this column name. this is only needed because of the column taxmap_obj$data$diff_table_2lineages$log2_median_ratio we created in chunk 3.5.3 
names(taxmap_obj$data$diff_table_3treatments)[4]<-"log2_median_ratio_stress"

3.7.2 - Plot a matrix of heat trees

Now that we have log-fold differences in pariwise treatment comparisons, let’s compare them in a matrix of heat trees

#plot matrix tree
set.seed(1)
heat_tree_matrix(taxmap_obj,
                 data = "diff_table_3treatments", # this is the table with the data you want to plot
                 node_size = n_obs, # n_obs is a function that calculates, in this case, the number of OTUs per taxon
                 node_label = taxon_names,
                 node_color = log2_median_ratio_stress, # A column from `taxmap_obj$data$diff_table_3treatments`
                 node_color_range = diverging_palette(), # The built-in palette for diverging data
                 node_color_trans = "linear", # The default is scaled by circle area
                 node_color_interval = c(-3, 3), # The range of `log2_median_ratio` to display
                 edge_color_interval = c(-3, 3), # The range of `log2_median_ratio` to display
                 node_size_axis_label = "Number of OTUs",
                 node_color_axis_label = "Log2 ratio median proportions",
                 layout = "davidson-harel", # The primary layout algorithm
                 initial_layout = "reingold-tilford", # The layout algorithm that initializes node locations
                 output_file = "./results/matrix_heat_tree_Stress.pdf") # Saves the plot as a pdf file

3.7.3 - EXERCISE change grouping variable

by changing the code above, Make a matrix of heat trees that plots the differences between the 3 plant species we are using. The metadata for that is present in the metadata column “sp_full_name”. which alphaproteobacteria are more common in Brassica oleracea than Isatis tinctoria?

QUESTION BREAK

3.8 - create a matrix of heat trees from a phyloseq object with a single function

These trees are interesting, but as you start exploring real data and multiple options you will need a more efficiency way of running all this code. for this, we create a custom function that handles all of it at once,

3.8.1 - Define a custom function

this custom function will:

1 - truncate the taxonomy table to genus level,
2 - remove the g__ and f__ etc from taxonomy table 3 - parse the phyloseq object into a taxmap object, 4 - calculate taxa occurence, abudance and log-fold differences between groups of samples that you define 5 - plot a heat tree

It’s arguments are 1 - ps_object = a phyloseq object 2 - sample_group = a (quoted) column from the sample metadata that you want to compare

The function will count the number of different groups in your metadata column to decide if it will plot a heat tree (2 treatments/groups) or matrix of heat trees (3+ treatments). the function should fail if your metadata only has 1 sample group!

phyloseq_to_heat_tree_matrix<-function(ps_object, sample_group){
  
  # this function output is a heat trees comparing metadata. the input is based on a phyloseq object
  
  # ps object =  a phyloseq object, containing sample metadata, otu table, and taxonomy table
  # sample_group = the name of a column in your emtadata that you want to compare in the heat tree. it has to be quoted.
  
  # this function will create a matrix of heat trees if your metadata has more than 2 groups. it should fail if it has only 1 group
  

#remove unecessary taxonomic info (dada2id, "s__" and "ASV_id) by updating the tax table with a subset of the tax table
tax_table(ps_object)<-tax_table(ps_object)[,1:6]


# let's remove the "r__"ranks from the taxonomy, they can be useful but will pollute our plot
tax_table(ps_object)[, colnames(tax_table(ps_object))] <- gsub(pattern = "[a-z]__", # regular expression pattern to search for
                                                                   x = tax_table(ps_object)[, colnames(tax_table(ps_object))], # "df"
                                                                   replacement = "") # replacement for pattern
# transform from phyloseq to taxmap object
taxmap_obj<-parse_phyloseq(ps_object)

#get abundance per taxon
taxmap_obj$data$tax_abund<-calc_taxon_abund(obj = taxmap_obj, 
                                      data = "otu_table",
                                      cols = taxmap_obj$data$sample_data$sample_id) 

#get occurrence of ASVs per treatment
# the sample groups needs some wrangling to fit within the soft code of the function
taxmap_obj$data$tax_occ<- calc_n_samples(obj = taxmap_obj, 
                                                      data = "tax_abund", 
                                                      cols = taxmap_obj$data$sample_data$sample_id,
                                                      groups = taxmap_obj$data$sample_data[colnames(taxmap_obj$data$sample_data)==sample_group][[1]]) 

# calculate log2 median ratios and p values for a wilcoxcon test within taxas in this stress treatment groups
# the sample groups needs some wrangling to fit within the soft code of the function
taxmap_obj$data$diff_table <- compare_groups(obj = taxmap_obj,
                                                        data = "tax_abund",
                                                        cols = taxmap_obj$data$sample_data$sample_id, 
                                                        groups = taxmap_obj$data$sample_data[colnames(taxmap_obj$data$sample_data)==sample_group][[1]]) 

# set differential log ratio to 0 based on adjusted p values
taxmap_obj$data$diff_table$log2_median_ratio[taxmap_obj$data$diff_table$wilcox_p_value > 0.05] <- 0

# define number of compared factors
factors_compared<-taxmap_obj$data$sample_data[colnames(taxmap_obj$data$sample_data)==sample_group][[1]] 

# draw the plot based on an if else statement: if there are 2 groups, plot a a heat tree comparing abundances between both groups, else plot a matrix of ehat trees. this function will fail if you only have 1 sample group! 

if (length(unique(factors_compared)) == 2) {

set.seed(1)
 output<- taxmap_obj %>%
heat_tree(
            node_label = taxon_names,
      #      data = "diff_table", 
            node_size = n_obs,
            node_color = log2_median_ratio,
            node_color_interval = c(-3, 3), # The range of `log2_median_ratio` to display
            node_color_range = c("cyan", "gray", "tan"), # The color palette used
            layout = "davidson-harel",
            initial_layout = "reingold-tilford")

 } else {

set.seed(1)
output<-heat_tree_matrix(taxmap_obj,
                         data = "diff_table", # this is the table with the data you want to plot
                         node_size = n_obs, # n_obs is a function that calculates, in this case, the number of OTUs per taxon
                         node_label = taxon_names,
                         node_color = log2_median_ratio, # A column from `taxmap_obj$data$diff_table_3treatments`
                         node_color_range = diverging_palette(), # The built-in palette for diverging data
                         node_color_interval = c(-3, 3), # The range of `log2_median_ratio` to display
                         edge_color_interval = c(-3, 3), # The range of `log2_median_ratio` to display
                         node_size_axis_label = "Number of OTUs",
                         node_color_axis_label = "Log2 ratio median proportions",
                         layout = "davidson-harel", # The primary layout algorithm
                         initial_layout = "reingold-tilford") # The layout algorithm that initializes node locations

   
   
 }


# clearly define the output object you will get from the function
return(output)   


}

3.8.2 - creating heat trees from a custom function

Now that we defined a new function that takes a phyloseq object as a data input and a column of the metadata as an argument, we can more easely produce heat trees across the variables.

# run custom function
heat_tree_stress<-phyloseq_to_heat_tree_matrix(ps_object = ps_rarefied, sample_group = "Stress")
heat_tree_lineage<-phyloseq_to_heat_tree_matrix(ps_object = ps_rarefied, sample_group = "Sp_Lineage_Walden")

# check new trees
heat_tree_stress

heat_tree_lineage

3.8.3 - EXERCISE make more heat trees!

Now that you know the basics, let’s manipulate some of these visualizations

3.8.3.1 - EXERCISE use custom function

Now produce new heat tree, based on metadata like “Sp_full_name”, “greenhouse_compartment” or “Speed”!

3.8.3.2 - EXERCISE see Burkholderiales and Rhizobiales at ASV level

Our trees so far stopped at genus level, because ploting 2000 ASVs would overcrowd the plot. Show the “Sp_full_name” effect at ASV level, but only on Burkholderiales and Rhizobiales. What do you have to change, and where? You can copy-paste and adjust the code we saw above, or make a new version of the custom function

3.8.3.3 - EXERCISE remove kingdom from taxonomy table

Our trees so far have k__Bacteria as a shared root. What will happen if you remove that taxonomic information? to make this change, What do you have to alter? You can copy-paste and adjust the code we saw above, or make a new version of the custom function

4.0 - create a list of taxmap objects for a single heat tree

Now that we have a function that takes a single phyloseq object as input, let’s run it across all the species in the dataset

4.1 - create a list of phyloseq objects

First, let’s split the phyloseq object into a list of phyloseq objects according the species

note that the phyloseq input is based on the original phyloseq object, that still contains the ranks as p__Proteobacteria, o__Rhizobiales, etc

# turn a ps object into a list f ps objects
ps_l<- metagMisc::phyloseq_sep_variable(ps_rarefied, variable = "Sp_abb_name")

4.2 - check list of phyloseq objects

Let’s check the list we just created. there are different ways we can do this

# check object, which is a list of phyloseq objects (ps_l)
ps_l
## $At
## phyloseq-class experiment-level object
## otu_table()   OTU Table:         [ 963 taxa and 48 samples ]
## sample_data() Sample Data:       [ 48 samples by 44 sample variables ]
## tax_table()   Taxonomy Table:    [ 963 taxa by 8 taxonomic ranks ]
## refseq()      DNAStringSet:      [ 963 reference sequences ]
## 
## $Bo_M
## phyloseq-class experiment-level object
## otu_table()   OTU Table:         [ 994 taxa and 48 samples ]
## sample_data() Sample Data:       [ 48 samples by 44 sample variables ]
## tax_table()   Taxonomy Table:    [ 994 taxa by 8 taxonomic ranks ]
## refseq()      DNAStringSet:      [ 994 reference sequences ]
## 
## $It
## phyloseq-class experiment-level object
## otu_table()   OTU Table:         [ 988 taxa and 47 samples ]
## sample_data() Sample Data:       [ 47 samples by 44 sample variables ]
## tax_table()   Taxonomy Table:    [ 988 taxa by 8 taxonomic ranks ]
## refseq()      DNAStringSet:      [ 988 reference sequences ]
# access the Arabidosps phyloseq object
ps_l$At
## phyloseq-class experiment-level object
## otu_table()   OTU Table:         [ 963 taxa and 48 samples ]
## sample_data() Sample Data:       [ 48 samples by 44 sample variables ]
## tax_table()   Taxonomy Table:    [ 963 taxa by 8 taxonomic ranks ]
## refseq()      DNAStringSet:      [ 963 reference sequences ]
# access the taxonomy table of the  Arabidosps phyloseq object
ps_l$At@tax_table
## Taxonomy Table:     [963 taxa by 8 taxonomic ranks]:
##           Kingdom       Phylum                 Class                   
## bASV_5    "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_7    "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_9    "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_11   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_12   "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_13   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_14   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_15   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_16   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_18   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_19   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_20   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_21   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_23   "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_25   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_27   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_29   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_30   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_31   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_32   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_33   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_34   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_35   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_36   "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_37   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_38   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_39   "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_40   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_41   "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_43   "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_44   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_45   "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_46   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_48   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_49   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_50   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_51   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_52   "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_53   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_54   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_55   "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_56   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_57   "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_58   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_59   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_60   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_61   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_62   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_63   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_64   "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_65   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_66   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_67   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_68   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_69   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_70   "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_71   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_72   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_73   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_74   "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_75   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_76   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_77   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_78   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_79   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_80   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_81   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_82   "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_83   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_84   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_85   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_86   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_87   "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_88   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_89   "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_90   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_91   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_92   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_93   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_94   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_95   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_96   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_97   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_98   "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_99   "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_100  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_101  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_102  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_103  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_104  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_105  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_106  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_107  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_108  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_109  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_110  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_111  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_112  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_113  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_114  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_115  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_116  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_117  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_118  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_119  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_120  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_121  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_122  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_123  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_124  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_125  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_126  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_128  "k__Bacteria" "p__Myxococcota"       "c__Polyangia"          
## bASV_129  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_131  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_132  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_133  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_134  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_135  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_136  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_137  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_138  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_139  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_140  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_141  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_142  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_143  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_144  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_145  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_146  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_147  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_148  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_149  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_151  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_152  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_153  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_154  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_155  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_156  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_158  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_159  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_160  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_161  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_162  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_163  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_164  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_165  "k__Bacteria" "p__Myxococcota"       "c__Polyangia"          
## bASV_166  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_168  "k__Bacteria" "p__Firmicutes"        "c__Bacilli"            
## bASV_169  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_170  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_171  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_173  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_174  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_175  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_176  "k__Bacteria" "p__Methylomirabilota" "c__Methylomirabilia"   
## bASV_177  "k__Bacteria" "p__Armatimonadota"    "c__Fimbriimonadia"     
## bASV_178  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_180  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_181  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_182  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_183  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_184  "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_185  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_186  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_187  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_188  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_189  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_190  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_191  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_192  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_193  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_194  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_195  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_196  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_197  "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_198  "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_199  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_200  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_201  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_202  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_203  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_204  "k__Bacteria" "p__Patescibacteria"   "c__Saccharimonadia"    
## bASV_205  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_206  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_207  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_209  "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_211  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_212  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_213  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_214  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_215  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_216  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_217  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_218  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_219  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_220  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_221  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_222  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_223  "k__Bacteria" NA                     NA                      
## bASV_224  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_225  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_226  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_227  "k__Bacteria" "p__Acidobacteriota"   "c__Holophagae"         
## bASV_228  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_229  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_230  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_231  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_232  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_233  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_234  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_235  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_237  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_238  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_240  "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_241  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_242  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_243  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_244  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_245  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_246  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_247  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_249  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_250  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_251  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_252  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_253  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_254  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_255  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_256  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_257  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_258  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_259  "k__Bacteria" "p__Chloroflexi"       "c__Chloroflexia"       
## bASV_260  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_261  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_262  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_263  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_264  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_265  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_266  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_268  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_269  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_270  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_271  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_272  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_273  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_274  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_275  "k__Bacteria" "p__Chloroflexi"       "c__Gitt-GS-136"        
## bASV_276  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_277  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_278  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_279  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_280  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_281  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_282  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_283  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_285  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_286  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_288  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_289  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_290  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_291  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_292  "k__Bacteria" "p__Firmicutes"        "c__Bacilli"            
## bASV_293  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_294  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_295  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_297  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_298  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_299  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_300  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_302  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_303  "k__Bacteria" "p__Patescibacteria"   "c__Saccharimonadia"    
## bASV_304  "k__Bacteria" "p__Nitrospirota"      "c__Nitrospiria"        
## bASV_305  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_306  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_307  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_308  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_309  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_310  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_312  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_313  "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_314  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_315  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_316  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_317  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_318  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_319  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_321  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_322  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_323  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_324  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_325  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_326  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_327  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_328  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_330  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_331  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_332  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_333  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_334  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_335  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_336  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_337  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_339  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_340  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_341  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_342  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_343  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_345  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_346  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_347  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_348  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_349  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_350  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_351  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_352  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_353  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_354  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_355  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_356  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_357  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_358  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_359  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_361  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_362  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_363  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_364  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_365  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_366  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_367  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_368  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_369  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_370  "k__Bacteria" "p__Patescibacteria"   "c__Saccharimonadia"    
## bASV_371  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_372  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_374  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_375  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_376  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_377  "k__Bacteria" "p__Chloroflexi"       "c__Gitt-GS-136"        
## bASV_378  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_379  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_380  "k__Bacteria" "p__Myxococcota"       "c__Myxococcia"         
## bASV_381  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_382  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_383  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_384  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_385  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_386  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_387  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_388  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_389  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_390  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_391  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_392  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_395  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_396  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_397  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_399  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_400  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_401  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_402  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_403  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_404  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_406  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_407  "k__Bacteria" "p__Armatimonadota"    "c__Fimbriimonadia"     
## bASV_408  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_409  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_410  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_411  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_412  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_413  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_414  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_416  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_417  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_418  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_419  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_420  "k__Bacteria" "p__Actinobacteriota"  "c__Thermoleophilia"    
## bASV_421  "k__Bacteria" "p__Gemmatimonadota"   "c__Longimicrobia"      
## bASV_422  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_423  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_424  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_425  "k__Bacteria" "p__Actinobacteriota"  "c__Acidimicrobiia"     
## bASV_426  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_427  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_428  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_429  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_430  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_431  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_432  "k__Bacteria" "p__Gemmatimonadota"   "c__Longimicrobia"      
## bASV_433  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_434  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_435  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_436  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_438  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_439  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_440  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_441  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_442  "k__Bacteria" "p__Chloroflexi"       "c__Chloroflexia"       
## bASV_443  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_446  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_447  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_448  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_449  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_450  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_451  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_453  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_454  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_455  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_456  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_457  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_458  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_459  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_460  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_461  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_462  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_464  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_465  "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_466  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_467  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_468  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_469  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_471  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_472  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_474  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_476  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_477  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_478  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_479  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_480  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_481  "k__Bacteria" "p__Myxococcota"       "c__Polyangia"          
## bASV_482  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_483  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_484  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_485  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_486  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_487  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_488  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_489  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_490  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_491  "k__Bacteria" "p__Firmicutes"        "c__Bacilli"            
## bASV_492  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_493  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_494  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_495  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_496  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_498  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_499  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_500  "k__Bacteria" "p__Actinobacteriota"  "c__Thermoleophilia"    
## bASV_501  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_503  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_504  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_505  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_506  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_507  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_508  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_509  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_510  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_511  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_512  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_513  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_514  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_515  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_516  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_517  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_518  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_519  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_520  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_521  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_522  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_524  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_525  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_526  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_527  "k__Bacteria" "p__Actinobacteriota"  "c__Acidimicrobiia"     
## bASV_528  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_529  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_530  "k__Bacteria" "p__Actinobacteriota"  "c__Acidimicrobiia"     
## bASV_531  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_533  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_534  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_535  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_536  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_537  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_539  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_540  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_541  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_542  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_546  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_547  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_548  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_549  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_550  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_551  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_552  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_553  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_554  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_556  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_557  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_558  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_561  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_562  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_563  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_564  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_565  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_567  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_569  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_570  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_571  "k__Bacteria" "p__Actinobacteriota"  "c__Acidimicrobiia"     
## bASV_572  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_573  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_574  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_575  "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_576  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_577  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_578  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_579  "k__Bacteria" "p__Chloroflexi"       "c__KD4-96"             
## bASV_580  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_581  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_582  "k__Bacteria" "p__Methylomirabilota" "c__Methylomirabilia"   
## bASV_583  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_584  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_585  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_586  "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_587  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_590  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_591  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_592  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_593  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_595  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_596  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_597  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_598  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_599  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_600  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_601  "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_602  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_603  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_604  "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_605  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_606  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_607  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_609  "k__Bacteria" "p__Acidobacteriota"   "c__Holophagae"         
## bASV_611  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_612  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_613  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_614  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_615  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_616  "k__Bacteria" "p__Actinobacteriota"  "c__Thermoleophilia"    
## bASV_617  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_619  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_621  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_622  "k__Bacteria" "p__Acidobacteriota"   "c__Holophagae"         
## bASV_623  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_624  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_625  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_626  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_627  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_628  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_629  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_632  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_635  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_636  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_637  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_638  "k__Bacteria" "p__Myxococcota"       "c__Polyangia"          
## bASV_639  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_640  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_641  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_642  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_643  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_645  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_646  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_647  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_648  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_649  "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_650  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_651  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_652  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_653  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_654  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_655  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_657  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_658  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_659  "k__Bacteria" "p__Actinobacteriota"  "c__Thermoleophilia"    
## bASV_660  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_661  "k__Bacteria" "p__Actinobacteriota"  "c__Acidimicrobiia"     
## bASV_662  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_663  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_664  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_665  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_666  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_667  "k__Bacteria" "p__Actinobacteriota"  "c__MB-A2-108"          
## bASV_668  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_669  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_670  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_672  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_674  "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_675  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_676  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_677  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_678  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_682  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_683  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_684  "k__Bacteria" "p__Firmicutes"        "c__Bacilli"            
## bASV_687  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_688  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_691  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_692  "k__Bacteria" "p__Chloroflexi"       "c__AD3"                
## bASV_693  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_694  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_695  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_696  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_698  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_699  "k__Bacteria" "p__Chloroflexi"       "c__KD4-96"             
## bASV_700  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_701  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_702  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_703  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_704  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_705  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_706  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_707  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_708  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_709  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_711  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_712  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_713  "k__Bacteria" "p__Planctomycetota"   "c__Planctomycetes"     
## bASV_714  "k__Bacteria" "p__Chloroflexi"       "c__Chloroflexia"       
## bASV_715  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_716  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_717  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_719  "k__Bacteria" "p__Firmicutes"        "c__Bacilli"            
## bASV_720  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_721  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_722  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_724  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_725  "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_726  "k__Bacteria" "p__Actinobacteriota"  "c__Acidimicrobiia"     
## bASV_728  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_729  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_730  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_731  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_732  "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_733  "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_735  "k__Bacteria" "p__Armatimonadota"    "c__Fimbriimonadia"     
## bASV_736  "k__Bacteria" "p__Nitrospirota"      "c__Nitrospiria"        
## bASV_737  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_738  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_739  "k__Bacteria" "p__Firmicutes"        "c__Bacilli"            
## bASV_740  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_741  "k__Bacteria" "p__Actinobacteriota"  "c__Acidimicrobiia"     
## bASV_743  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_744  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_745  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_746  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_747  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_749  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_752  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_753  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_754  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_755  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_756  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_757  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_758  "k__Bacteria" "p__Myxococcota"       "c__Polyangia"          
## bASV_759  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_760  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_761  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_762  "k__Bacteria" "p__Acidobacteriota"   "c__Holophagae"         
## bASV_763  "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_764  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_766  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_767  "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_769  "k__Bacteria" "p__Acidobacteriota"   "c__Holophagae"         
## bASV_770  "k__Bacteria" "p__Myxococcota"       "c__Polyangia"          
## bASV_771  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_772  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_773  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_776  "k__Bacteria" "p__Myxococcota"       "c__Polyangia"          
## bASV_777  "k__Bacteria" "p__Nitrospirota"      "c__Nitrospiria"        
## bASV_780  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_782  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_784  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_785  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_786  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_787  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_788  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_789  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_791  "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_792  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_793  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_794  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_795  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_797  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_798  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_799  "k__Bacteria" "p__Chloroflexi"       "c__Ktedonobacteria"    
## bASV_800  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_801  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_803  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_804  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_805  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_806  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_807  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_808  "k__Bacteria" "p__Chloroflexi"       "c__KD4-96"             
## bASV_809  "k__Bacteria" "p__Firmicutes"        "c__Bacilli"            
## bASV_810  "k__Bacteria" "p__Myxococcota"       "c__Polyangia"          
## bASV_812  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_813  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_814  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_816  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_817  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_819  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_820  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_821  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_822  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_823  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_824  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_825  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_826  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_827  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_828  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_829  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_830  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_831  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_832  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_833  "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_835  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_836  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_837  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_838  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_840  "k__Bacteria" "p__Actinobacteriota"  "c__Acidimicrobiia"     
## bASV_841  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_842  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_843  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_844  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_845  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_846  "k__Bacteria" NA                     NA                      
## bASV_847  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_848  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_849  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_851  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_854  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_855  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_856  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_857  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_858  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_859  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_860  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_861  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_865  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_866  "k__Bacteria" "p__Firmicutes"        "c__Bacilli"            
## bASV_867  "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_868  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_869  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_870  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_871  "k__Bacteria" "p__Firmicutes"        "c__Bacilli"            
## bASV_874  "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_875  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_876  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_877  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_878  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_879  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_880  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_882  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_883  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_885  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_886  "k__Bacteria" "p__Deinococcota"      "c__Deinococci"         
## bASV_887  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_888  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_890  "k__Bacteria" "p__Actinobacteriota"  "c__Acidimicrobiia"     
## bASV_891  "k__Bacteria" "p__Myxococcota"       "c__Myxococcia"         
## bASV_892  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_893  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_895  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_896  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_897  "k__Bacteria" "p__Actinobacteriota"  "c__Thermoleophilia"    
## bASV_898  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_901  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_902  "k__Bacteria" "p__Actinobacteriota"  "c__Acidimicrobiia"     
## bASV_903  "k__Bacteria" "p__Planctomycetota"   "c__Planctomycetes"     
## bASV_904  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_905  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_906  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_907  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_908  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_909  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_910  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_911  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_912  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_914  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_915  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_916  "k__Bacteria" "p__Patescibacteria"   "c__Saccharimonadia"    
## bASV_917  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_918  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_920  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_921  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_922  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_923  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_924  "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_925  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_926  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_928  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_930  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_931  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_933  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_934  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_935  "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_936  "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_937  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_939  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_940  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_941  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_945  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_946  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_947  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_948  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_949  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_950  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_951  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_952  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_953  "k__Bacteria" "p__Patescibacteria"   "c__Saccharimonadia"    
## bASV_954  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_955  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_956  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_958  "k__Bacteria" "p__Actinobacteriota"  "c__Acidimicrobiia"     
## bASV_959  "k__Bacteria" "p__Actinobacteriota"  "c__Acidimicrobiia"     
## bASV_960  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_961  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_963  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_964  "k__Bacteria" "p__Myxococcota"       "c__Polyangia"          
## bASV_966  "k__Bacteria" "p__Chloroflexi"       "c__Ktedonobacteria"    
## bASV_969  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_972  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_973  "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_974  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_975  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_976  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_977  "k__Bacteria" "p__Acidobacteriota"   "c__Holophagae"         
## bASV_979  "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_980  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_981  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_982  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_983  "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_984  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_986  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_988  "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_989  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_990  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_991  "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_992  "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_994  "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_995  "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_996  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_998  "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_999  "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1001 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1003 "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_1005 "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_1006 "k__Bacteria" "p__Methylomirabilota" "c__Methylomirabilia"   
## bASV_1007 "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_1008 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1010 "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_1011 "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_1012 "k__Bacteria" "p__Actinobacteriota"  "c__Acidimicrobiia"     
## bASV_1013 "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_1014 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1015 "k__Bacteria" "p__Firmicutes"        "c__Bacilli"            
## bASV_1016 "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_1017 "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_1018 "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_1019 "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_1020 "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_1021 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1022 "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_1027 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1028 "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_1029 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1030 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1031 "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_1032 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1033 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1034 "k__Bacteria" "p__Patescibacteria"   "c__Saccharimonadia"    
## bASV_1035 "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_1037 "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_1038 "k__Bacteria" "p__Firmicutes"        "c__Bacilli"            
## bASV_1039 "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_1041 "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_1042 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1045 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1046 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1047 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1048 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1049 "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_1052 "k__Bacteria" "p__Chloroflexi"       "c__Ktedonobacteria"    
## bASV_1054 "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_1055 "k__Bacteria" "p__Myxococcota"       "c__Polyangia"          
## bASV_1056 "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_1057 "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_1058 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1059 "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_1060 "k__Bacteria" "p__Patescibacteria"   "c__Saccharimonadia"    
## bASV_1061 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1062 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1064 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1066 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1067 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1068 "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_1070 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1071 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1072 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1073 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1074 "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_1075 "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_1077 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1078 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1079 "k__Bacteria" "p__Myxococcota"       "c__Polyangia"          
## bASV_1080 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1081 "k__Bacteria" "p__Acidobacteriota"   "c__Holophagae"         
## bASV_1082 "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_1083 "k__Bacteria" "p__Myxococcota"       "c__Polyangia"          
## bASV_1084 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1085 "k__Bacteria" "p__Cyanobacteria"     "c__Vampirivibrionia"   
## bASV_1086 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1087 "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_1088 "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_1089 "k__Bacteria" "p__Chloroflexi"       "c__Chloroflexia"       
## bASV_1090 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1091 "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_1092 "k__Bacteria" "p__Nitrospirota"      "c__Nitrospiria"        
## bASV_1094 "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_1095 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1096 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1098 "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_1099 "k__Bacteria" "p__Gemmatimonadota"   "c__Gemmatimonadetes"   
## bASV_1102 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1103 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1105 "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_1107 "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_1108 "k__Bacteria" "p__Verrucomicrobiota" "c__Verrucomicrobiae"   
## bASV_1109 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1110 "k__Bacteria" "p__Bacteroidota"      "c__Bacteroidia"        
## bASV_1111 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1112 "k__Bacteria" "p__Actinobacteriota"  "c__Actinobacteria"     
## bASV_1114 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1115 "k__Bacteria" "p__Acidobacteriota"   "c__Acidobacteriae"     
## bASV_1116 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1117 "k__Bacteria" "p__Bdellovibrionota"  "c__Bdellovibrionia"    
## bASV_1118 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1119 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1120 "k__Bacteria" "p__Actinobacteriota"  "c__Acidimicrobiia"     
## bASV_1121 "k__Bacteria" "p__Acidobacteriota"   "c__Holophagae"         
## bASV_1122 "k__Bacteria" "p__Chloroflexi"       "c__Ktedonobacteria"    
## bASV_1123 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1124 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1125 "k__Bacteria" "p__Planctomycetota"   "c__Phycisphaerae"      
## bASV_1126 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1127 "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_1128 "k__Bacteria" "p__Proteobacteria"    "c__Alphaproteobacteria"
## bASV_1129 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1130 "k__Bacteria" "p__Planctomycetota"   "c__Planctomycetes"     
## bASV_1131 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1132 "k__Bacteria" "p__Acidobacteriota"   "c__Vicinamibacteria"   
## bASV_1133 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
## bASV_1134 "k__Bacteria" "p__Myxococcota"       "c__Polyangia"          
## bASV_1136 "k__Bacteria" "p__Acidobacteriota"   "c__Blastocatellia"     
## bASV_1137 "k__Bacteria" "p__Proteobacteria"    "c__Gammaproteobacteria"
##           Order                                  
## bASV_5    "o__Burkholderiales"                   
## bASV_7    "o__Pseudomonadales"                   
## bASV_9    "o__Chitinophagales"                   
## bASV_11   "o__Sphingomonadales"                  
## bASV_12   "o__Streptomycetales"                  
## bASV_13   "o__Rhizobiales"                       
## bASV_14   "o__Burkholderiales"                   
## bASV_15   "o__Burkholderiales"                   
## bASV_16   "o__Burkholderiales"                   
## bASV_18   "o__Rhizobiales"                       
## bASV_19   "o__Caulobacterales"                   
## bASV_20   "o__Burkholderiales"                   
## bASV_21   "o__Sphingomonadales"                  
## bASV_23   "o__Chitinophagales"                   
## bASV_25   "o__Burkholderiales"                   
## bASV_27   "o__Burkholderiales"                   
## bASV_29   "o__Rhizobiales"                       
## bASV_30   "o__Caulobacterales"                   
## bASV_31   "o__Rhizobiales"                       
## bASV_32   "o__Burkholderiales"                   
## bASV_33   "o__Pseudomonadales"                   
## bASV_34   "o__Burkholderiales"                   
## bASV_35   "o__Burkholderiales"                   
## bASV_36   "o__Chitinophagales"                   
## bASV_37   "o__Burkholderiales"                   
## bASV_38   "o__Burkholderiales"                   
## bASV_39   "o__Chitinophagales"                   
## bASV_40   "o__Burkholderiales"                   
## bASV_41   "o__Streptomycetales"                  
## bASV_43   "o__Micrococcales"                     
## bASV_44   "o__Xanthomonadales"                   
## bASV_45   "o__Chitinophagales"                   
## bASV_46   "o__Burkholderiales"                   
## bASV_48   "o__Rhizobiales"                       
## bASV_49   "o__Sphingomonadales"                  
## bASV_50   "o__Rhizobiales"                       
## bASV_51   "o__Burkholderiales"                   
## bASV_52   "o__Streptomycetales"                  
## bASV_53   "o__Rhizobiales"                       
## bASV_54   "o__Burkholderiales"                   
## bASV_55   "o__Streptomycetales"                  
## bASV_56   "o__Burkholderiales"                   
## bASV_57   "o__Micrococcales"                     
## bASV_58   "o__Burkholderiales"                   
## bASV_59   "o__Burkholderiales"                   
## bASV_60   "o__Sphingomonadales"                  
## bASV_61   "o__Rhizobiales"                       
## bASV_62   "o__Sphingomonadales"                  
## bASV_63   "o__Rhizobiales"                       
## bASV_64   "o__Cytophagales"                      
## bASV_65   "o__Burkholderiales"                   
## bASV_66   "o__Rhizobiales"                       
## bASV_67   "o__Rhizobiales"                       
## bASV_68   "o__Burkholderiales"                   
## bASV_69   "o__Rhizobiales"                       
## bASV_70   "o__Chitinophagales"                   
## bASV_71   "o__Xanthomonadales"                   
## bASV_72   "o__Burkholderiales"                   
## bASV_73   "o__Caulobacterales"                   
## bASV_74   "o__Chitinophagales"                   
## bASV_75   "o__Burkholderiales"                   
## bASV_76   "o__Caulobacterales"                   
## bASV_77   "o__Burkholderiales"                   
## bASV_78   "o__Sphingomonadales"                  
## bASV_79   "o__Burkholderiales"                   
## bASV_80   "o__Xanthomonadales"                   
## bASV_81   "o__Burkholderiales"                   
## bASV_82   "o__Streptomycetales"                  
## bASV_83   "o__Burkholderiales"                   
## bASV_84   "o__Burkholderiales"                   
## bASV_85   "o__Burkholderiales"                   
## bASV_86   "o__Burkholderiales"                   
## bASV_87   "o__Chitinophagales"                   
## bASV_88   "o__Rhizobiales"                       
## bASV_89   "o__Rhizobiales"                       
## bASV_90   "o__Xanthomonadales"                   
## bASV_91   "o__Xanthomonadales"                   
## bASV_92   "o__Burkholderiales"                   
## bASV_93   "o__Burkholderiales"                   
## bASV_94   "o__Burkholderiales"                   
## bASV_95   "o__Burkholderiales"                   
## bASV_96   "o__Xanthomonadales"                   
## bASV_97   "o__Burkholderiales"                   
## bASV_98   "o__Acidobacteriales"                  
## bASV_99   "o__Xanthomonadales"                   
## bASV_100  "o__Caulobacterales"                   
## bASV_101  "o__Pseudomonadales"                   
## bASV_102  "o__Chitinophagales"                   
## bASV_103  "o__Caulobacterales"                   
## bASV_104  "o__Burkholderiales"                   
## bASV_105  "o__Burkholderiales"                   
## bASV_106  "o__Xanthomonadales"                   
## bASV_107  "o__Sphingomonadales"                  
## bASV_108  "o__Chitinophagales"                   
## bASV_109  "o__Chitinophagales"                   
## bASV_110  "o__Sphingomonadales"                  
## bASV_111  "o__Rhizobiales"                       
## bASV_112  "o__Burkholderiales"                   
## bASV_113  "o__Burkholderiales"                   
## bASV_114  "o__Sphingomonadales"                  
## bASV_115  "o__Caulobacterales"                   
## bASV_116  "o__Xanthomonadales"                   
## bASV_117  "o__Burkholderiales"                   
## bASV_118  "o__Gemmatimonadales"                  
## bASV_119  "o__Burkholderiales"                   
## bASV_120  "o__Gemmatimonadales"                  
## bASV_121  "o__Pseudonocardiales"                 
## bASV_122  "o__Xanthomonadales"                   
## bASV_123  "o__Chitinophagales"                   
## bASV_124  "o__Propionibacteriales"               
## bASV_125  "o__Gemmatimonadales"                  
## bASV_126  "o__Xanthomonadales"                   
## bASV_128  "o__Nannocystales"                     
## bASV_129  "o__Burkholderiales"                   
## bASV_131  "o__Burkholderiales"                   
## bASV_132  "o__Xanthomonadales"                   
## bASV_133  "o__Rhizobiales"                       
## bASV_134  "o__Burkholderiales"                   
## bASV_135  "o__Sphingomonadales"                  
## bASV_136  "o__Burkholderiales"                   
## bASV_137  "o__Rhizobiales"                       
## bASV_138  "o__Bryobacterales"                    
## bASV_139  "o__Burkholderiales"                   
## bASV_140  "o__Burkholderiales"                   
## bASV_141  "o__Caulobacterales"                   
## bASV_142  "o__Xanthomonadales"                   
## bASV_143  "o__Burkholderiales"                   
## bASV_144  "o__Rhizobiales"                       
## bASV_145  "o__Burkholderiales"                   
## bASV_146  "o__Rhizobiales"                       
## bASV_147  "o__Sphingomonadales"                  
## bASV_148  "o__Burkholderiales"                   
## bASV_149  "o__Sphingomonadales"                  
## bASV_151  "o__Burkholderiales"                   
## bASV_152  "o__Sphingomonadales"                  
## bASV_153  "o__Burkholderiales"                   
## bASV_154  "o__Flavobacteriales"                  
## bASV_155  "o__Rhizobiales"                       
## bASV_156  "o__Burkholderiales"                   
## bASV_158  "o__Burkholderiales"                   
## bASV_159  "o__Sphingobacteriales"                
## bASV_160  "o__Sphingomonadales"                  
## bASV_161  "o__Sphingomonadales"                  
## bASV_162  "o__Caulobacterales"                   
## bASV_163  "o__Sphingomonadales"                  
## bASV_164  "o__Xanthomonadales"                   
## bASV_165  "o__MSB-4B10"                          
## bASV_166  "o__Burkholderiales"                   
## bASV_168  "o__Bacillales"                        
## bASV_169  "o__Chitinophagales"                   
## bASV_170  "o__Azospirillales"                    
## bASV_171  "o__Pyrinomonadales"                   
## bASV_173  "o__Rhizobiales"                       
## bASV_174  "o__Burkholderiales"                   
## bASV_175  "o__Burkholderiales"                   
## bASV_176  "o__Rokubacteriales"                   
## bASV_177  "o__Fimbriimonadales"                  
## bASV_178  "o__Caulobacterales"                   
## bASV_180  "o__Sphingomonadales"                  
## bASV_181  "o__Propionibacteriales"               
## bASV_182  "o__Steroidobacterales"                
## bASV_183  "o__Pseudonocardiales"                 
## bASV_184  "o__Chthoniobacterales"                
## bASV_185  "o__Gemmatimonadales"                  
## bASV_186  "o__Burkholderiales"                   
## bASV_187  "o__Sphingomonadales"                  
## bASV_188  "o__Xanthomonadales"                   
## bASV_189  "o__Rhizobiales"                       
## bASV_190  "o__Sphingomonadales"                  
## bASV_191  "o__Rhizobiales"                       
## bASV_192  "o__Burkholderiales"                   
## bASV_193  "o__Sphingomonadales"                  
## bASV_194  "o__Sphingomonadales"                  
## bASV_195  "o__Burkholderiales"                   
## bASV_196  "o__Xanthomonadales"                   
## bASV_197  "o__Verrucomicrobiales"                
## bASV_198  "o__Tepidisphaerales"                  
## bASV_199  "o__Burkholderiales"                   
## bASV_200  "o__Gemmatimonadales"                  
## bASV_201  "o__Rhizobiales"                       
## bASV_202  "o__Xanthomonadales"                   
## bASV_203  "o__Cytophagales"                      
## bASV_204  "o__Saccharimonadales"                 
## bASV_205  "o__Xanthomonadales"                   
## bASV_206  "o__Sphingomonadales"                  
## bASV_207  "o__Rhizobiales"                       
## bASV_209  "o__Chthoniobacterales"                
## bASV_211  "o__Caulobacterales"                   
## bASV_212  "o__Burkholderiales"                   
## bASV_213  "o__Burkholderiales"                   
## bASV_214  "o__Gemmatimonadales"                  
## bASV_215  "o__Micrococcales"                     
## bASV_216  "o__Sphingobacteriales"                
## bASV_217  "o__Xanthomonadales"                   
## bASV_218  "o__Burkholderiales"                   
## bASV_219  "o__Rhizobiales"                       
## bASV_220  "o__Burkholderiales"                   
## bASV_221  "o__Sphingobacteriales"                
## bASV_222  "o__Micrococcales"                     
## bASV_223  NA                                     
## bASV_224  "o__Burkholderiales"                   
## bASV_225  "o__Cytophagales"                      
## bASV_226  "o__Rhizobiales"                       
## bASV_227  "o__Subgroup_7"                        
## bASV_228  "o__Xanthomonadales"                   
## bASV_229  "o__Gemmatimonadales"                  
## bASV_230  "o__Burkholderiales"                   
## bASV_231  "o__Sphingomonadales"                  
## bASV_232  "o__Burkholderiales"                   
## bASV_233  "o__Chitinophagales"                   
## bASV_234  "o__Blastocatellales"                  
## bASV_235  "o__Xanthomonadales"                   
## bASV_237  "o__Burkholderiales"                   
## bASV_238  "o__Sphingomonadales"                  
## bASV_240  "o__Tepidisphaerales"                  
## bASV_241  "o__Pseudonocardiales"                 
## bASV_242  "o__Chitinophagales"                   
## bASV_243  "o__Burkholderiales"                   
## bASV_244  "o__Gemmatimonadales"                  
## bASV_245  "o__Streptomycetales"                  
## bASV_246  "o__Streptomycetales"                  
## bASV_247  "o__Chitinophagales"                   
## bASV_249  "o__Micromonosporales"                 
## bASV_250  "o__Burkholderiales"                   
## bASV_251  "o__Sphingomonadales"                  
## bASV_252  "o__Sphingomonadales"                  
## bASV_253  "o__Burkholderiales"                   
## bASV_254  "o__Blastocatellales"                  
## bASV_255  "o__Burkholderiales"                   
## bASV_256  "o__Sphingomonadales"                  
## bASV_257  "o__Rhizobiales"                       
## bASV_258  "o__Burkholderiales"                   
## bASV_259  "o__Chloroflexales"                    
## bASV_260  "o__Burkholderiales"                   
## bASV_261  "o__Acidobacteriales"                  
## bASV_262  "o__Sphingomonadales"                  
## bASV_263  "o__Cytophagales"                      
## bASV_264  "o__Burkholderiales"                   
## bASV_265  "o__Gemmatimonadales"                  
## bASV_266  "o__Burkholderiales"                   
## bASV_268  "o__Chitinophagales"                   
## bASV_269  "o__Burkholderiales"                   
## bASV_270  "o__Steroidobacterales"                
## bASV_271  "o__Reyranellales"                     
## bASV_272  "o__Gemmatimonadales"                  
## bASV_273  "o__Burkholderiales"                   
## bASV_274  "o__Burkholderiales"                   
## bASV_275  "o__Gitt-GS-136"                       
## bASV_276  "o__Burkholderiales"                   
## bASV_277  "o__Burkholderiales"                   
## bASV_278  "o__Burkholderiales"                   
## bASV_279  "o__Gemmatimonadales"                  
## bASV_280  "o__Chitinophagales"                   
## bASV_281  "o__Rhizobiales"                       
## bASV_282  "o__Caulobacterales"                   
## bASV_283  "o__Caulobacterales"                   
## bASV_285  "o__Burkholderiales"                   
## bASV_286  "o__Rhizobiales"                       
## bASV_288  "o__Xanthomonadales"                   
## bASV_289  "o__Burkholderiales"                   
## bASV_290  "o__Burkholderiales"                   
## bASV_291  "o__Pseudonocardiales"                 
## bASV_292  "o__Bacillales"                        
## bASV_293  "o__Burkholderiales"                   
## bASV_294  "o__Blastocatellales"                  
## bASV_295  "o__Burkholderiales"                   
## bASV_297  "o__Caulobacterales"                   
## bASV_298  "o__Rhizobiales"                       
## bASV_299  "o__Blastocatellales"                  
## bASV_300  "o__Burkholderiales"                   
## bASV_302  "o__Burkholderiales"                   
## bASV_303  "o__Saccharimonadales"                 
## bASV_304  "o__Nitrospirales"                     
## bASV_305  "o__Xanthomonadales"                   
## bASV_306  "o__Burkholderiales"                   
## bASV_307  "o__Burkholderiales"                   
## bASV_308  "o__Burkholderiales"                   
## bASV_309  "o__Burkholderiales"                   
## bASV_310  "o__Caulobacterales"                   
## bASV_312  "o__Burkholderiales"                   
## bASV_313  "o__Verrucomicrobiales"                
## bASV_314  "o__Burkholderiales"                   
## bASV_315  "o__Rhizobiales"                       
## bASV_316  "o__Gemmatimonadales"                  
## bASV_317  "o__Sphingomonadales"                  
## bASV_318  "o__Sphingomonadales"                  
## bASV_319  "o__Gammaproteobacteria_Incertae_Sedis"
## bASV_321  "o__Sphingomonadales"                  
## bASV_322  "o__Xanthomonadales"                   
## bASV_323  "o__Sphingobacteriales"                
## bASV_324  "o__Cytophagales"                      
## bASV_325  "o__Burkholderiales"                   
## bASV_326  "o__Blastocatellales"                  
## bASV_327  "o__Xanthomonadales"                   
## bASV_328  "o__Burkholderiales"                   
## bASV_330  "o__Chitinophagales"                   
## bASV_331  "o__Xanthomonadales"                   
## bASV_332  "o__Gemmatimonadales"                  
## bASV_333  "o__Cytophagales"                      
## bASV_334  "o__Sphingobacteriales"                
## bASV_335  "o__Propionibacteriales"               
## bASV_336  "o__Burkholderiales"                   
## bASV_337  "o__Burkholderiales"                   
## bASV_339  "o__Rhizobiales"                       
## bASV_340  "o__Sphingomonadales"                  
## bASV_341  "o__Burkholderiales"                   
## bASV_342  "o__Burkholderiales"                   
## bASV_343  "o__Burkholderiales"                   
## bASV_345  "o__Chitinophagales"                   
## bASV_346  "o__Burkholderiales"                   
## bASV_347  "o__Cellvibrionales"                   
## bASV_348  "o__Sphingomonadales"                  
## bASV_349  "o__Burkholderiales"                   
## bASV_350  "o__Sphingomonadales"                  
## bASV_351  "o__Rhizobiales"                       
## bASV_352  "o__Sphingomonadales"                  
## bASV_353  "o__Caulobacterales"                   
## bASV_354  "o__Chitinophagales"                   
## bASV_355  "o__Sphingomonadales"                  
## bASV_356  "o__Burkholderiales"                   
## bASV_357  "o__Burkholderiales"                   
## bASV_358  "o__Sphingobacteriales"                
## bASV_359  "o__Caulobacterales"                   
## bASV_361  "o__Sphingomonadales"                  
## bASV_362  "o__Burkholderiales"                   
## bASV_363  "o__Cytophagales"                      
## bASV_364  "o__Rhizobiales"                       
## bASV_365  "o__Chitinophagales"                   
## bASV_366  "o__Burkholderiales"                   
## bASV_367  "o__Gemmatimonadales"                  
## bASV_368  "o__Rhizobiales"                       
## bASV_369  "o__Burkholderiales"                   
## bASV_370  "o__Saccharimonadales"                 
## bASV_371  "o__Xanthomonadales"                   
## bASV_372  "o__Burkholderiales"                   
## bASV_374  "o__Gemmatimonadales"                  
## bASV_375  "o__Burkholderiales"                   
## bASV_376  "o__Burkholderiales"                   
## bASV_377  "o__Gitt-GS-136"                       
## bASV_378  "o__Gemmatimonadales"                  
## bASV_379  "o__Burkholderiales"                   
## bASV_380  "o__Myxococcales"                      
## bASV_381  "o__Xanthomonadales"                   
## bASV_382  "o__Burkholderiales"                   
## bASV_383  "o__Gemmatimonadales"                  
## bASV_384  "o__Burkholderiales"                   
## bASV_385  "o__Propionibacteriales"               
## bASV_386  "o__Sphingomonadales"                  
## bASV_387  "o__Chitinophagales"                   
## bASV_388  "o__Rhizobiales"                       
## bASV_389  "o__Burkholderiales"                   
## bASV_390  "o__Burkholderiales"                   
## bASV_391  "o__Bryobacterales"                    
## bASV_392  "o__Reyranellales"                     
## bASV_395  "o__Gemmatimonadales"                  
## bASV_396  "o__Burkholderiales"                   
## bASV_397  "o__Gemmatimonadales"                  
## bASV_399  "o__Burkholderiales"                   
## bASV_400  "o__Burkholderiales"                   
## bASV_401  "o__Rhizobiales"                       
## bASV_402  "o__Rhizobiales"                       
## bASV_403  "o__Burkholderiales"                   
## bASV_404  "o__Burkholderiales"                   
## bASV_406  "o__Xanthomonadales"                   
## bASV_407  "o__Fimbriimonadales"                  
## bASV_408  "o__Blastocatellales"                  
## bASV_409  "o__Propionibacteriales"               
## bASV_410  "o__Burkholderiales"                   
## bASV_411  "o__Xanthomonadales"                   
## bASV_412  "o__Burkholderiales"                   
## bASV_413  "o__Xanthomonadales"                   
## bASV_414  "o__Pseudomonadales"                   
## bASV_416  "o__Burkholderiales"                   
## bASV_417  "o__Rhizobiales"                       
## bASV_418  "o__Rhizobiales"                       
## bASV_419  "o__Burkholderiales"                   
## bASV_420  "o__Gaiellales"                        
## bASV_421  "o__Longimicrobiales"                  
## bASV_422  "o__Sphingomonadales"                  
## bASV_423  "o__Burkholderiales"                   
## bASV_424  "o__Xanthomonadales"                   
## bASV_425  "o__Microtrichales"                    
## bASV_426  "o__Sphingomonadales"                  
## bASV_427  "o__Vicinamibacterales"                
## bASV_428  "o__Gammaproteobacteria_Incertae_Sedis"
## bASV_429  "o__Chitinophagales"                   
## bASV_430  "o__Caulobacterales"                   
## bASV_431  "o__Cytophagales"                      
## bASV_432  "o__Longimicrobiales"                  
## bASV_433  "o__Steroidobacterales"                
## bASV_434  "o__Propionibacteriales"               
## bASV_435  "o__Acidobacteriales"                  
## bASV_436  "o__Cytophagales"                      
## bASV_438  "o__Rhizobiales"                       
## bASV_439  "o__Micropepsales"                     
## bASV_440  "o__Burkholderiales"                   
## bASV_441  "o__Burkholderiales"                   
## bASV_442  "o__Chloroflexales"                    
## bASV_443  "o__Cytophagales"                      
## bASV_446  "o__Sphingomonadales"                  
## bASV_447  "o__Blastocatellales"                  
## bASV_448  "o__Chitinophagales"                   
## bASV_449  "o__Rhizobiales"                       
## bASV_450  "o__Rhizobiales"                       
## bASV_451  "o__Dongiales"                         
## bASV_453  "o__Burkholderiales"                   
## bASV_454  "o__Frankiales"                        
## bASV_455  "o__Sphingobacteriales"                
## bASV_456  "o__Propionibacteriales"               
## bASV_457  "o__Burkholderiales"                   
## bASV_458  "o__Propionibacteriales"               
## bASV_459  "o__Sphingobacteriales"                
## bASV_460  "o__Propionibacteriales"               
## bASV_461  "o__Sphingomonadales"                  
## bASV_462  "o__Sphingomonadales"                  
## bASV_464  "o__Gemmatimonadales"                  
## bASV_465  "o__Tepidisphaerales"                  
## bASV_466  "o__Pseudonocardiales"                 
## bASV_467  "o__Vicinamibacterales"                
## bASV_468  "o__Gemmatimonadales"                  
## bASV_469  "o__Burkholderiales"                   
## bASV_471  "o__Burkholderiales"                   
## bASV_472  "o__Chitinophagales"                   
## bASV_474  "o__Burkholderiales"                   
## bASV_476  "o__Burkholderiales"                   
## bASV_477  "o__Burkholderiales"                   
## bASV_478  "o__Burkholderiales"                   
## bASV_479  "o__Xanthomonadales"                   
## bASV_480  "o__Streptosporangiales"               
## bASV_481  "o__Polyangiales"                      
## bASV_482  "o__Burkholderiales"                   
## bASV_483  "o__Micrococcales"                     
## bASV_484  "o__Burkholderiales"                   
## bASV_485  "o__Burkholderiales"                   
## bASV_486  "o__Chitinophagales"                   
## bASV_487  "o__Burkholderiales"                   
## bASV_488  "o__Xanthomonadales"                   
## bASV_489  "o__Corynebacteriales"                 
## bASV_490  "o__Xanthomonadales"                   
## bASV_491  "o__Bacillales"                        
## bASV_492  "o__uncultured"                        
## bASV_493  "o__Flavobacteriales"                  
## bASV_494  "o__Chitinophagales"                   
## bASV_495  "o__Burkholderiales"                   
## bASV_496  "o__Gammaproteobacteria_Incertae_Sedis"
## bASV_498  "o__Pyrinomonadales"                   
## bASV_499  "o__Sphingomonadales"                  
## bASV_500  "o__Gaiellales"                        
## bASV_501  "o__Solibacterales"                    
## bASV_503  "o__Blastocatellales"                  
## bASV_504  "o__Xanthomonadales"                   
## bASV_505  "o__Propionibacteriales"               
## bASV_506  "o__Sphingomonadales"                  
## bASV_507  "o__Burkholderiales"                   
## bASV_508  "o__Dongiales"                         
## bASV_509  "o__Dongiales"                         
## bASV_510  "o__Sphingomonadales"                  
## bASV_511  "o__Burkholderiales"                   
## bASV_512  "o__Cellvibrionales"                   
## bASV_513  "o__Blastocatellales"                  
## bASV_514  "o__Burkholderiales"                   
## bASV_515  "o__Caulobacterales"                   
## bASV_516  "o__Reyranellales"                     
## bASV_517  "o__Flavobacteriales"                  
## bASV_518  "o__Burkholderiales"                   
## bASV_519  "o__Bryobacterales"                    
## bASV_520  "o__Gemmatimonadales"                  
## bASV_521  "o__Burkholderiales"                   
## bASV_522  "o__Chitinophagales"                   
## bASV_524  "o__Sphingomonadales"                  
## bASV_525  "o__Burkholderiales"                   
## bASV_526  "o__Rhizobiales"                       
## bASV_527  "o__IMCC26256"                         
## bASV_528  "o__Burkholderiales"                   
## bASV_529  "o__Burkholderiales"                   
## bASV_530  "o__IMCC26256"                         
## bASV_531  "o__Gemmatimonadales"                  
## bASV_533  "o__Flavobacteriales"                  
## bASV_534  "o__Blastocatellales"                  
## bASV_535  "o__Flavobacteriales"                  
## bASV_536  "o__Propionibacteriales"               
## bASV_537  "o__Burkholderiales"                   
## bASV_539  "o__Burkholderiales"                   
## bASV_540  "o__Burkholderiales"                   
## bASV_541  "o__Sphingomonadales"                  
## bASV_542  "o__Glycomycetales"                    
## bASV_546  "o__Acidobacteriales"                  
## bASV_547  "o__Sphingobacteriales"                
## bASV_548  "o__Xanthomonadales"                   
## bASV_549  "o__Gemmatimonadales"                  
## bASV_550  "o__Burkholderiales"                   
## bASV_551  "o__Pyrinomonadales"                   
## bASV_552  "o__Xanthomonadales"                   
## bASV_553  "o__Sphingobacteriales"                
## bASV_554  "o__Streptosporangiales"               
## bASV_556  "o__Propionibacteriales"               
## bASV_557  "o__Xanthomonadales"                   
## bASV_558  "o__Chitinophagales"                   
## bASV_561  "o__Xanthomonadales"                   
## bASV_562  "o__Caulobacterales"                   
## bASV_563  "o__Burkholderiales"                   
## bASV_564  "o__Chitinophagales"                   
## bASV_565  "o__Sphingomonadales"                  
## bASV_567  "o__Caulobacterales"                   
## bASV_569  "o__Flavobacteriales"                  
## bASV_570  "o__Chitinophagales"                   
## bASV_571  "o__IMCC26256"                         
## bASV_572  "o__Sphingomonadales"                  
## bASV_573  "o__Rhizobiales"                       
## bASV_574  "o__Vicinamibacterales"                
## bASV_575  "o__Tepidisphaerales"                  
## bASV_576  "o__Acidobacteriales"                  
## bASV_577  "o__Gemmatimonadales"                  
## bASV_578  "o__Solibacterales"                    
## bASV_579  "o__KD4-96"                            
## bASV_580  "o__Burkholderiales"                   
## bASV_581  "o__Rhizobiales"                       
## bASV_582  "o__Rokubacteriales"                   
## bASV_583  "o__Solibacterales"                    
## bASV_584  "o__Caulobacterales"                   
## bASV_585  "o__Vicinamibacterales"                
## bASV_586  "o__Tepidisphaerales"                  
## bASV_587  "o__Burkholderiales"                   
## bASV_590  "o__Burkholderiales"                   
## bASV_591  "o__Micropepsales"                     
## bASV_592  "o__Burkholderiales"                   
## bASV_593  "o__Pseudonocardiales"                 
## bASV_595  "o__Gemmatimonadales"                  
## bASV_596  "o__Xanthomonadales"                   
## bASV_597  "o__Gammaproteobacteria_Incertae_Sedis"
## bASV_598  "o__Micrococcales"                     
## bASV_599  "o__Rhizobiales"                       
## bASV_600  "o__Sphingobacteriales"                
## bASV_601  "o__Tepidisphaerales"                  
## bASV_602  "o__Rhizobiales"                       
## bASV_603  "o__Burkholderiales"                   
## bASV_604  "o__Tepidisphaerales"                  
## bASV_605  "o__Xanthomonadales"                   
## bASV_606  "o__Burkholderiales"                   
## bASV_607  "o__Gemmatimonadales"                  
## bASV_609  "o__Subgroup_7"                        
## bASV_611  "o__Sphingomonadales"                  
## bASV_612  "o__Vicinamibacterales"                
## bASV_613  "o__Gemmatimonadales"                  
## bASV_614  "o__Propionibacteriales"               
## bASV_615  "o__Burkholderiales"                   
## bASV_616  "o__Gaiellales"                        
## bASV_617  "o__Rhizobiales"                       
## bASV_619  "o__Burkholderiales"                   
## bASV_621  "o__Rhizobiales"                       
## bASV_622  "o__Subgroup_7"                        
## bASV_623  "o__Burkholderiales"                   
## bASV_624  "o__Sphingomonadales"                  
## bASV_625  "o__Burkholderiales"                   
## bASV_626  "o__Burkholderiales"                   
## bASV_627  "o__Burkholderiales"                   
## bASV_628  "o__Chitinophagales"                   
## bASV_629  "o__Vicinamibacterales"                
## bASV_632  "o__Micropepsales"                     
## bASV_635  "o__Sphingomonadales"                  
## bASV_636  "o__Xanthomonadales"                   
## bASV_637  "o__Gemmatimonadales"                  
## bASV_638  "o__Haliangiales"                      
## bASV_639  "o__Sphingomonadales"                  
## bASV_640  "o__Burkholderiales"                   
## bASV_641  "o__Rhizobiales"                       
## bASV_642  "o__Micrococcales"                     
## bASV_643  "o__Pyrinomonadales"                   
## bASV_645  "o__Caulobacterales"                   
## bASV_646  "o__Burkholderiales"                   
## bASV_647  "o__Sphingomonadales"                  
## bASV_648  "o__Pyrinomonadales"                   
## bASV_649  "o__Chthoniobacterales"                
## bASV_650  "o__Burkholderiales"                   
## bASV_651  "o__Sphingobacteriales"                
## bASV_652  "o__Xanthomonadales"                   
## bASV_653  "o__Gemmatimonadales"                  
## bASV_654  "o__Dongiales"                         
## bASV_655  "o__Flavobacteriales"                  
## bASV_657  "o__Burkholderiales"                   
## bASV_658  "o__Micrococcales"                     
## bASV_659  "o__Solirubrobacterales"               
## bASV_660  "o__Vicinamibacterales"                
## bASV_661  "o__IMCC26256"                         
## bASV_662  "o__Burkholderiales"                   
## bASV_663  "o__Burkholderiales"                   
## bASV_664  "o__Rhizobiales"                       
## bASV_665  "o__Caulobacterales"                   
## bASV_666  "o__Gemmatimonadales"                  
## bASV_667  "o__MB-A2-108"                         
## bASV_668  "o__PLTA13"                            
## bASV_669  "o__Burkholderiales"                   
## bASV_670  "o__Subgroup_17"                       
## bASV_672  "o__Burkholderiales"                   
## bASV_674  "o__Tepidisphaerales"                  
## bASV_675  "o__Vicinamibacterales"                
## bASV_676  "o__Blastocatellales"                  
## bASV_677  "o__Burkholderiales"                   
## bASV_678  "o__Sphingomonadales"                  
## bASV_682  "o__Caulobacterales"                   
## bASV_683  "o__Glycomycetales"                    
## bASV_684  "o__Paenibacillales"                   
## bASV_687  "o__uncultured"                        
## bASV_688  "o__Gammaproteobacteria_Incertae_Sedis"
## bASV_691  "o__Subgroup_2"                        
## bASV_692  "o__AD3"                               
## bASV_693  "o__Frankiales"                        
## bASV_694  "o__Burkholderiales"                   
## bASV_695  "o__Propionibacteriales"               
## bASV_696  "o__Steroidobacterales"                
## bASV_698  "o__Rhizobiales"                       
## bASV_699  "o__KD4-96"                            
## bASV_700  "o__Gemmatimonadales"                  
## bASV_701  "o__Burkholderiales"                   
## bASV_702  "o__Frankiales"                        
## bASV_703  "o__Burkholderiales"                   
## bASV_704  "o__Gemmatimonadales"                  
## bASV_705  "o__Propionibacteriales"               
## bASV_706  "o__Chitinophagales"                   
## bASV_707  "o__Vicinamibacterales"                
## bASV_708  "o__Sphingobacteriales"                
## bASV_709  "o__Gammaproteobacteria_Incertae_Sedis"
## bASV_711  "o__Propionibacteriales"               
## bASV_712  "o__Burkholderiales"                   
## bASV_713  "o__Planctomycetales"                  
## bASV_714  "o__Chloroflexales"                    
## bASV_715  "o__Pseudonocardiales"                 
## bASV_716  "o__Burkholderiales"                   
## bASV_717  "o__Chitinophagales"                   
## bASV_719  "o__Paenibacillales"                   
## bASV_720  "o__Gemmatimonadales"                  
## bASV_721  "o__Chitinophagales"                   
## bASV_722  "o__Burkholderiales"                   
## bASV_724  "o__Flavobacteriales"                  
## bASV_725  "o__Chthoniobacterales"                
## bASV_726  "o__IMCC26256"                         
## bASV_728  "o__Sphingomonadales"                  
## bASV_729  "o__Xanthomonadales"                   
## bASV_730  "o__Burkholderiales"                   
## bASV_731  "o__Burkholderiales"                   
## bASV_732  "o__Chthoniobacterales"                
## bASV_733  "o__Chthoniobacterales"                
## bASV_735  "o__Fimbriimonadales"                  
## bASV_736  "o__Nitrospirales"                     
## bASV_737  "o__Acidobacteriales"                  
## bASV_738  "o__Rhizobiales"                       
## bASV_739  "o__Paenibacillales"                   
## bASV_740  "o__Gemmatimonadales"                  
## bASV_741  "o__Microtrichales"                    
## bASV_743  "o__Chitinophagales"                   
## bASV_744  "o__Burkholderiales"                   
## bASV_745  "o__Burkholderiales"                   
## bASV_746  "o__Rhizobiales"                       
## bASV_747  "o__Gemmatimonadales"                  
## bASV_749  "o__Rhizobiales"                       
## bASV_752  "o__Sphingomonadales"                  
## bASV_753  "o__Vicinamibacterales"                
## bASV_754  "o__Burkholderiales"                   
## bASV_755  "o__Burkholderiales"                   
## bASV_756  "o__Burkholderiales"                   
## bASV_757  "o__Caulobacterales"                   
## bASV_758  "o__Polyangiales"                      
## bASV_759  "o__Rhizobiales"                       
## bASV_760  "o__Sphingomonadales"                  
## bASV_761  "o__Vicinamibacterales"                
## bASV_762  "o__Subgroup_7"                        
## bASV_763  "o__Tepidisphaerales"                  
## bASV_764  "o__Gemmatimonadales"                  
## bASV_766  "o__Sphingomonadales"                  
## bASV_767  "o__Opitutales"                        
## bASV_769  "o__Subgroup_7"                        
## bASV_770  "o__Polyangiales"                      
## bASV_771  "o__Micrococcales"                     
## bASV_772  "o__Burkholderiales"                   
## bASV_773  "o__Rhizobiales"                       
## bASV_776  "o__mle1-27"                           
## bASV_777  "o__Nitrospirales"                     
## bASV_780  "o__Burkholderiales"                   
## bASV_782  "o__Propionibacteriales"               
## bASV_784  "o__Xanthomonadales"                   
## bASV_785  "o__Burkholderiales"                   
## bASV_786  "o__Burkholderiales"                   
## bASV_787  "o__Chitinophagales"                   
## bASV_788  "o__Pseudonocardiales"                 
## bASV_789  "o__Burkholderiales"                   
## bASV_791  "o__Pedosphaerales"                    
## bASV_792  "o__Burkholderiales"                   
## bASV_793  "o__Chitinophagales"                   
## bASV_794  "o__Burkholderiales"                   
## bASV_795  "o__Gemmatimonadales"                  
## bASV_797  "o__Streptomycetales"                  
## bASV_798  "o__Sphingomonadales"                  
## bASV_799  "o__C0119"                             
## bASV_800  "o__Gemmatimonadales"                  
## bASV_801  "o__Burkholderiales"                   
## bASV_803  "o__Chitinophagales"                   
## bASV_804  "o__Burkholderiales"                   
## bASV_805  "o__Burkholderiales"                   
## bASV_806  "o__Chitinophagales"                   
## bASV_807  "o__Pyrinomonadales"                   
## bASV_808  "o__KD4-96"                            
## bASV_809  "o__Bacillales"                        
## bASV_810  "o__Polyangiales"                      
## bASV_812  "o__Chitinophagales"                   
## bASV_813  "o__Sphingomonadales"                  
## bASV_814  "o__Rhizobiales"                       
## bASV_816  "o__Gammaproteobacteria_Incertae_Sedis"
## bASV_817  "o__Burkholderiales"                   
## bASV_819  "o__Propionibacteriales"               
## bASV_820  "o__Burkholderiales"                   
## bASV_821  "o__Gemmatimonadales"                  
## bASV_822  "o__Acidobacteriales"                  
## bASV_823  "o__Gemmatimonadales"                  
## bASV_824  "o__Propionibacteriales"               
## bASV_825  "o__Burkholderiales"                   
## bASV_826  "o__Burkholderiales"                   
## bASV_827  "o__Xanthomonadales"                   
## bASV_828  "o__Burkholderiales"                   
## bASV_829  "o__Micropepsales"                     
## bASV_830  "o__Subgroup_17"                       
## bASV_831  "o__Burkholderiales"                   
## bASV_832  "o__Xanthomonadales"                   
## bASV_833  "o__Chthoniobacterales"                
## bASV_835  "o__Sphingomonadales"                  
## bASV_836  "o__Burkholderiales"                   
## bASV_837  "o__Pyrinomonadales"                   
## bASV_838  "o__Xanthomonadales"                   
## bASV_840  "o__Microtrichales"                    
## bASV_841  "o__Burkholderiales"                   
## bASV_842  "o__Cytophagales"                      
## bASV_843  "o__Burkholderiales"                   
## bASV_844  "o__Burkholderiales"                   
## bASV_845  "o__Burkholderiales"                   
## bASV_846  NA                                     
## bASV_847  "o__Burkholderiales"                   
## bASV_848  "o__Cytophagales"                      
## bASV_849  "o__Vicinamibacterales"                
## bASV_851  "o__Flavobacteriales"                  
## bASV_854  "o__Burkholderiales"                   
## bASV_855  "o__Rhizobiales"                       
## bASV_856  "o__Vicinamibacterales"                
## bASV_857  "o__Burkholderiales"                   
## bASV_858  "o__Chitinophagales"                   
## bASV_859  "o__Caulobacterales"                   
## bASV_860  "o__Burkholderiales"                   
## bASV_861  "o__Blastocatellales"                  
## bASV_865  "o__Gemmatimonadales"                  
## bASV_866  "o__Bacillales"                        
## bASV_867  "o__Tepidisphaerales"                  
## bASV_868  "o__Burkholderiales"                   
## bASV_869  "o__Pyrinomonadales"                   
## bASV_870  "o__Burkholderiales"                   
## bASV_871  "o__Paenibacillales"                   
## bASV_874  "o__Opitutales"                        
## bASV_875  "o__Xanthomonadales"                   
## bASV_876  "o__Burkholderiales"                   
## bASV_877  "o__Vicinamibacterales"                
## bASV_878  "o__Burkholderiales"                   
## bASV_879  "o__Burkholderiales"                   
## bASV_880  "o__Burkholderiales"                   
## bASV_882  "o__Burkholderiales"                   
## bASV_883  "o__Burkholderiales"                   
## bASV_885  "o__Rhizobiales"                       
## bASV_886  "o__Deinococcales"                     
## bASV_887  "o__Gemmatimonadales"                  
## bASV_888  "o__Burkholderiales"                   
## bASV_890  "o__uncultured"                        
## bASV_891  "o__Myxococcales"                      
## bASV_892  "o__Chitinophagales"                   
## bASV_893  "o__Burkholderiales"                   
## bASV_895  "o__Gemmatimonadales"                  
## bASV_896  "o__11-24"                             
## bASV_897  "o__Gaiellales"                        
## bASV_898  "o__Acidobacteriales"                  
## bASV_901  "o__Chitinophagales"                   
## bASV_902  "o__uncultured"                        
## bASV_903  "o__Gemmatales"                        
## bASV_904  "o__Flavobacteriales"                  
## bASV_905  "o__Pseudonocardiales"                 
## bASV_906  "o__Gemmatimonadales"                  
## bASV_907  "o__Pseudomonadales"                   
## bASV_908  "o__Caulobacterales"                   
## bASV_909  "o__Burkholderiales"                   
## bASV_910  "o__Micropepsales"                     
## bASV_911  "o__Burkholderiales"                   
## bASV_912  "o__Rhizobiales"                       
## bASV_914  "o__Burkholderiales"                   
## bASV_915  "o__Burkholderiales"                   
## bASV_916  "o__Saccharimonadales"                 
## bASV_917  "o__Vicinamibacterales"                
## bASV_918  "o__Flavobacteriales"                  
## bASV_920  "o__Gemmatimonadales"                  
## bASV_921  "o__Burkholderiales"                   
## bASV_922  "o__Rhizobiales"                       
## bASV_923  "o__Micromonosporales"                 
## bASV_924  "o__Tepidisphaerales"                  
## bASV_925  "o__Burkholderiales"                   
## bASV_926  "o__Rhizobiales"                       
## bASV_928  "o__Steroidobacterales"                
## bASV_930  "o__Xanthomonadales"                   
## bASV_931  "o__Solibacterales"                    
## bASV_933  "o__Sphingomonadales"                  
## bASV_934  "o__Rhizobiales"                       
## bASV_935  "o__Chthoniobacterales"                
## bASV_936  "o__Tepidisphaerales"                  
## bASV_937  "o__Flavobacteriales"                  
## bASV_939  "o__Rhizobiales"                       
## bASV_940  "o__Rhizobiales"                       
## bASV_941  "o__Burkholderiales"                   
## bASV_945  "o__Sphingomonadales"                  
## bASV_946  "o__Burkholderiales"                   
## bASV_947  "o__Burkholderiales"                   
## bASV_948  "o__Burkholderiales"                   
## bASV_949  "o__Burkholderiales"                   
## bASV_950  "o__Bryobacterales"                    
## bASV_951  "o__Rhizobiales"                       
## bASV_952  "o__Cytophagales"                      
## bASV_953  "o__Saccharimonadales"                 
## bASV_954  "o__Burkholderiales"                   
## bASV_955  "o__Rhizobiales"                       
## bASV_956  "o__Cytophagales"                      
## bASV_958  "o__Microtrichales"                    
## bASV_959  "o__IMCC26256"                         
## bASV_960  "o__Burkholderiales"                   
## bASV_961  "o__Solibacterales"                    
## bASV_963  "o__Gemmatimonadales"                  
## bASV_964  "o__Polyangiales"                      
## bASV_966  "o__C0119"                             
## bASV_969  "o__Elsterales"                        
## bASV_972  "o__Caulobacterales"                   
## bASV_973  "o__Tepidisphaerales"                  
## bASV_974  "o__Micropepsales"                     
## bASV_975  "o__Chitinophagales"                   
## bASV_976  "o__Burkholderiales"                   
## bASV_977  "o__Subgroup_7"                        
## bASV_979  "o__Pedosphaerales"                    
## bASV_980  "o__Burkholderiales"                   
## bASV_981  "o__Vicinamibacterales"                
## bASV_982  "o__Xanthomonadales"                   
## bASV_983  "o__Pyrinomonadales"                   
## bASV_984  "o__Rhizobiales"                       
## bASV_986  "o__Xanthomonadales"                   
## bASV_988  "o__Solibacterales"                    
## bASV_989  "o__Burkholderiales"                   
## bASV_990  "o__Rhizobiales"                       
## bASV_991  "o__Gemmatimonadales"                  
## bASV_992  "o__Vicinamibacterales"                
## bASV_994  "o__Chitinophagales"                   
## bASV_995  "o__Frankiales"                        
## bASV_996  "o__Xanthomonadales"                   
## bASV_998  "o__Rhizobiales"                       
## bASV_999  "o__Burkholderiales"                   
## bASV_1001 "o__Rhizobiales"                       
## bASV_1003 "o__Gemmatimonadales"                  
## bASV_1005 "o__Gemmatimonadales"                  
## bASV_1006 "o__Rokubacteriales"                   
## bASV_1007 "o__Blastocatellales"                  
## bASV_1008 "o__Rhizobiales"                       
## bASV_1010 "o__Gemmatimonadales"                  
## bASV_1011 "o__Pseudonocardiales"                 
## bASV_1012 "o__Microtrichales"                    
## bASV_1013 "o__Chitinophagales"                   
## bASV_1014 "o__Xanthomonadales"                   
## bASV_1015 "o__Bacillales"                        
## bASV_1016 "o__Gemmatimonadales"                  
## bASV_1017 "o__Chitinophagales"                   
## bASV_1018 "o__Gemmatimonadales"                  
## bASV_1019 "o__Verrucomicrobiales"                
## bASV_1020 "o__Tepidisphaerales"                  
## bASV_1021 "o__Burkholderiales"                   
## bASV_1022 "o__Vicinamibacterales"                
## bASV_1027 "o__Xanthomonadales"                   
## bASV_1028 "o__Tepidisphaerales"                  
## bASV_1029 "o__Burkholderiales"                   
## bASV_1030 "o__Xanthomonadales"                   
## bASV_1031 "o__Frankiales"                        
## bASV_1032 "o__Burkholderiales"                   
## bASV_1033 "o__Micropepsales"                     
## bASV_1034 "o__Saccharimonadales"                 
## bASV_1035 "o__Sphingobacteriales"                
## bASV_1037 "o__Chitinophagales"                   
## bASV_1038 "o__Paenibacillales"                   
## bASV_1039 "o__Verrucomicrobiales"                
## bASV_1041 "o__Vicinamibacterales"                
## bASV_1042 "o__Burkholderiales"                   
## bASV_1045 NA                                     
## bASV_1046 "o__Xanthomonadales"                   
## bASV_1047 "o__Burkholderiales"                   
## bASV_1048 "o__Burkholderiales"                   
## bASV_1049 "o__Tepidisphaerales"                  
## bASV_1052 "o__Ktedonobacterales"                 
## bASV_1054 "o__Flavobacteriales"                  
## bASV_1055 "o__Blfdi19"                           
## bASV_1056 "o__Propionibacteriales"               
## bASV_1057 "o__Chitinophagales"                   
## bASV_1058 "o__Rhizobiales"                       
## bASV_1059 "o__Solibacterales"                    
## bASV_1060 "o__Saccharimonadales"                 
## bASV_1061 "o__Rhizobiales"                       
## bASV_1062 NA                                     
## bASV_1064 "o__Burkholderiales"                   
## bASV_1066 "o__Micropepsales"                     
## bASV_1067 "o__Dongiales"                         
## bASV_1068 "o__Glycomycetales"                    
## bASV_1070 "o__Burkholderiales"                   
## bASV_1071 "o__Caulobacterales"                   
## bASV_1072 "o__Burkholderiales"                   
## bASV_1073 "o__Burkholderiales"                   
## bASV_1074 "o__Propionibacteriales"               
## bASV_1075 "o__Solibacterales"                    
## bASV_1077 "o__Burkholderiales"                   
## bASV_1078 "o__uncultured"                        
## bASV_1079 "o__Polyangiales"                      
## bASV_1080 "o__Pseudomonadales"                   
## bASV_1081 "o__Subgroup_7"                        
## bASV_1082 "o__Vicinamibacterales"                
## bASV_1083 "o__Haliangiales"                      
## bASV_1084 "o__Xanthomonadales"                   
## bASV_1085 "o__Vampirovibrionales"                
## bASV_1086 "o__Steroidobacterales"                
## bASV_1087 "o__Gemmatimonadales"                  
## bASV_1088 "o__Micrococcales"                     
## bASV_1089 "o__Chloroflexales"                    
## bASV_1090 "o__Burkholderiales"                   
## bASV_1091 "o__Gemmatimonadales"                  
## bASV_1092 "o__Nitrospirales"                     
## bASV_1094 "o__Verrucomicrobiales"                
## bASV_1095 "o__Acetobacterales"                   
## bASV_1096 "o__Xanthomonadales"                   
## bASV_1098 "o__Chitinophagales"                   
## bASV_1099 "o__Gemmatimonadales"                  
## bASV_1102 "o__Burkholderiales"                   
## bASV_1103 "o__Burkholderiales"                   
## bASV_1105 "o__Tepidisphaerales"                  
## bASV_1107 "o__Cytophagales"                      
## bASV_1108 "o__Opitutales"                        
## bASV_1109 "o__Burkholderiales"                   
## bASV_1110 "o__Sphingobacteriales"                
## bASV_1111 "o__Burkholderiales"                   
## bASV_1112 "o__Pseudonocardiales"                 
## bASV_1114 "o__Rhizobiales"                       
## bASV_1115 "o__Bryobacterales"                    
## bASV_1116 "o__Sphingomonadales"                  
## bASV_1117 "o__Bdellovibrionales"                 
## bASV_1118 "o__Caulobacterales"                   
## bASV_1119 "o__Sphingomonadales"                  
## bASV_1120 NA                                     
## bASV_1121 "o__Subgroup_7"                        
## bASV_1122 "o__C0119"                             
## bASV_1123 "o__Rhizobiales"                       
## bASV_1124 "o__Rhizobiales"                       
## bASV_1125 "o__Tepidisphaerales"                  
## bASV_1126 "o__Burkholderiales"                   
## bASV_1127 "o__Vicinamibacterales"                
## bASV_1128 "o__Rhizobiales"                       
## bASV_1129 "o__Burkholderiales"                   
## bASV_1130 "o__Planctomycetales"                  
## bASV_1131 "o__Gammaproteobacteria_Incertae_Sedis"
## bASV_1132 "o__Vicinamibacterales"                
## bASV_1133 "o__Burkholderiales"                   
## bASV_1134 "o__Polyangiales"                      
## bASV_1136 "o__Blastocatellales"                  
## bASV_1137 "o__Burkholderiales"                   
##           Family                             
## bASV_5    "f__Comamonadaceae"                
## bASV_7    "f__Pseudomonadaceae"              
## bASV_9    "f__Chitinophagaceae"              
## bASV_11   "f__Sphingomonadaceae"             
## bASV_12   "f__Streptomycetaceae"             
## bASV_13   "f__Rhizobiaceae"                  
## bASV_14   "f__Comamonadaceae"                
## bASV_15   "f__Oxalobacteraceae"              
## bASV_16   "f__Oxalobacteraceae"              
## bASV_18   "f__Xanthobacteraceae"             
## bASV_19   "f__Caulobacteraceae"              
## bASV_20   "f__Comamonadaceae"                
## bASV_21   "f__Sphingomonadaceae"             
## bASV_23   "f__Chitinophagaceae"              
## bASV_25   "f__Oxalobacteraceae"              
## bASV_27   "f__Comamonadaceae"                
## bASV_29   "f__Devosiaceae"                   
## bASV_30   "f__Caulobacteraceae"              
## bASV_31   "f__Devosiaceae"                   
## bASV_32   "f__Oxalobacteraceae"              
## bASV_33   "f__Pseudomonadaceae"              
## bASV_34   "f__Comamonadaceae"                
## bASV_35   "f__Oxalobacteraceae"              
## bASV_36   "f__Chitinophagaceae"              
## bASV_37   "f__Comamonadaceae"                
## bASV_38   "f__Oxalobacteraceae"              
## bASV_39   "f__Chitinophagaceae"              
## bASV_40   "f__Oxalobacteraceae"              
## bASV_41   "f__Streptomycetaceae"             
## bASV_43   "f__Micrococcaceae"                
## bASV_44   "f__Xanthomonadaceae"              
## bASV_45   "f__Chitinophagaceae"              
## bASV_46   "f__Comamonadaceae"                
## bASV_48   "f__Xanthobacteraceae"             
## bASV_49   "f__Sphingomonadaceae"             
## bASV_50   "f__Xanthobacteraceae"             
## bASV_51   "f__Oxalobacteraceae"              
## bASV_52   "f__Streptomycetaceae"             
## bASV_53   "f__Rhizobiaceae"                  
## bASV_54   "f__Comamonadaceae"                
## bASV_55   "f__Streptomycetaceae"             
## bASV_56   "f__Oxalobacteraceae"              
## bASV_57   "f__Micrococcaceae"                
## bASV_58   "f__Comamonadaceae"                
## bASV_59   "f__Comamonadaceae"                
## bASV_60   "f__Sphingomonadaceae"             
## bASV_61   "f__Rhizobiaceae"                  
## bASV_62   "f__Sphingomonadaceae"             
## bASV_63   "f__Rhizobiaceae"                  
## bASV_64   "f__Microscillaceae"               
## bASV_65   "f__Oxalobacteraceae"              
## bASV_66   "f__Rhizobiaceae"                  
## bASV_67   "f__Beijerinckiaceae"              
## bASV_68   "f__Comamonadaceae"                
## bASV_69   "f__Rhizobiaceae"                  
## bASV_70   "f__Chitinophagaceae"              
## bASV_71   "f__Rhodanobacteraceae"            
## bASV_72   "f__Comamonadaceae"                
## bASV_73   "f__Caulobacteraceae"              
## bASV_74   "f__Chitinophagaceae"              
## bASV_75   "f__Oxalobacteraceae"              
## bASV_76   "f__Caulobacteraceae"              
## bASV_77   "f__Comamonadaceae"                
## bASV_78   "f__Sphingomonadaceae"             
## bASV_79   "f__Oxalobacteraceae"              
## bASV_80   "f__Xanthomonadaceae"              
## bASV_81   "f__Comamonadaceae"                
## bASV_82   "f__Streptomycetaceae"             
## bASV_83   "f__Oxalobacteraceae"              
## bASV_84   "f__Methylophilaceae"              
## bASV_85   "f__Comamonadaceae"                
## bASV_86   "f__Oxalobacteraceae"              
## bASV_87   "f__Chitinophagaceae"              
## bASV_88   "f__Rhizobiaceae"                  
## bASV_89   "f__Rhizobiaceae"                  
## bASV_90   "f__Rhodanobacteraceae"            
## bASV_91   "f__Xanthomonadaceae"              
## bASV_92   "f__Oxalobacteraceae"              
## bASV_93   "f__Comamonadaceae"                
## bASV_94   "f__Oxalobacteraceae"              
## bASV_95   "f__Comamonadaceae"                
## bASV_96   "f__Xanthomonadaceae"              
## bASV_97   "f__Comamonadaceae"                
## bASV_98   "f__uncultured"                    
## bASV_99   "f__Rhodanobacteraceae"            
## bASV_100  "f__Caulobacteraceae"              
## bASV_101  "f__Pseudomonadaceae"              
## bASV_102  "f__Chitinophagaceae"              
## bASV_103  "f__Caulobacteraceae"              
## bASV_104  "f__Oxalobacteraceae"              
## bASV_105  "f__Comamonadaceae"                
## bASV_106  "f__Xanthomonadaceae"              
## bASV_107  "f__Sphingomonadaceae"             
## bASV_108  "f__Chitinophagaceae"              
## bASV_109  "f__Chitinophagaceae"              
## bASV_110  "f__Sphingomonadaceae"             
## bASV_111  "f__Rhizobiaceae"                  
## bASV_112  "f__Oxalobacteraceae"              
## bASV_113  "f__Comamonadaceae"                
## bASV_114  "f__Sphingomonadaceae"             
## bASV_115  "f__Caulobacteraceae"              
## bASV_116  "f__Xanthomonadaceae"              
## bASV_117  "f__Oxalobacteraceae"              
## bASV_118  "f__Gemmatimonadaceae"             
## bASV_119  "f__Oxalobacteraceae"              
## bASV_120  "f__Gemmatimonadaceae"             
## bASV_121  "f__Pseudonocardiaceae"            
## bASV_122  "f__Xanthomonadaceae"              
## bASV_123  "f__Chitinophagaceae"              
## bASV_124  "f__Nocardioidaceae"               
## bASV_125  "f__Gemmatimonadaceae"             
## bASV_126  "f__Xanthomonadaceae"              
## bASV_128  "f__Nannocystaceae"                
## bASV_129  "f__Comamonadaceae"                
## bASV_131  "f__Comamonadaceae"                
## bASV_132  "f__Xanthomonadaceae"              
## bASV_133  "f__Xanthobacteraceae"             
## bASV_134  "f__Oxalobacteraceae"              
## bASV_135  "f__Sphingomonadaceae"             
## bASV_136  "f__Oxalobacteraceae"              
## bASV_137  "f__Rhizobiaceae"                  
## bASV_138  "f__Bryobacteraceae"               
## bASV_139  "f__Burkholderiaceae"              
## bASV_140  "f__Oxalobacteraceae"              
## bASV_141  "f__Caulobacteraceae"              
## bASV_142  "f__Xanthomonadaceae"              
## bASV_143  "f__Comamonadaceae"                
## bASV_144  "f__Rhizobiaceae"                  
## bASV_145  "f__Oxalobacteraceae"              
## bASV_146  "f__Rhizobiaceae"                  
## bASV_147  "f__Sphingomonadaceae"             
## bASV_148  "f__Burkholderiaceae"              
## bASV_149  "f__Sphingomonadaceae"             
## bASV_151  "f__Oxalobacteraceae"              
## bASV_152  "f__Sphingomonadaceae"             
## bASV_153  "f__Oxalobacteraceae"              
## bASV_154  "f__Crocinitomicaceae"             
## bASV_155  "f__Devosiaceae"                   
## bASV_156  "f__Oxalobacteraceae"              
## bASV_158  "f__Comamonadaceae"                
## bASV_159  "f__Sphingobacteriaceae"           
## bASV_160  "f__Sphingomonadaceae"             
## bASV_161  "f__Sphingomonadaceae"             
## bASV_162  "f__Caulobacteraceae"              
## bASV_163  "f__Sphingomonadaceae"             
## bASV_164  "f__Xanthomonadaceae"              
## bASV_165  "f__MSB-4B10"                      
## bASV_166  "f__Oxalobacteraceae"              
## bASV_168  "f__Bacillaceae"                   
## bASV_169  "f__Chitinophagaceae"              
## bASV_170  "f__Azospirillaceae"               
## bASV_171  "f__Pyrinomonadaceae"              
## bASV_173  "f__Rhizobiaceae"                  
## bASV_174  "f__Comamonadaceae"                
## bASV_175  "f__Comamonadaceae"                
## bASV_176  "f__Rokubacteriales"               
## bASV_177  "f__Fimbriimonadaceae"             
## bASV_178  "f__Caulobacteraceae"              
## bASV_180  "f__Sphingomonadaceae"             
## bASV_181  "f__Nocardioidaceae"               
## bASV_182  "f__Steroidobacteraceae"           
## bASV_183  "f__Pseudonocardiaceae"            
## bASV_184  "f__Chthoniobacteraceae"           
## bASV_185  "f__Gemmatimonadaceae"             
## bASV_186  "f__Oxalobacteraceae"              
## bASV_187  "f__Sphingomonadaceae"             
## bASV_188  "f__Xanthomonadaceae"              
## bASV_189  "f__Rhizobiaceae"                  
## bASV_190  "f__Sphingomonadaceae"             
## bASV_191  "f__Xanthobacteraceae"             
## bASV_192  "f__Oxalobacteraceae"              
## bASV_193  "f__Sphingomonadaceae"             
## bASV_194  "f__Sphingomonadaceae"             
## bASV_195  "f__Nitrosomonadaceae"             
## bASV_196  "f__Rhodanobacteraceae"            
## bASV_197  "f__Rubritaleaceae"                
## bASV_198  "f__WD2101_soil_group"             
## bASV_199  "f__Oxalobacteraceae"              
## bASV_200  "f__Gemmatimonadaceae"             
## bASV_201  "f__Xanthobacteraceae"             
## bASV_202  "f__Xanthomonadaceae"              
## bASV_203  "f__Microscillaceae"               
## bASV_204  "f__Saccharimonadaceae"            
## bASV_205  "f__Xanthomonadaceae"              
## bASV_206  "f__Sphingomonadaceae"             
## bASV_207  "f__Devosiaceae"                   
## bASV_209  "f__Chthoniobacteraceae"           
## bASV_211  "f__Caulobacteraceae"              
## bASV_212  "f__Burkholderiaceae"              
## bASV_213  "f__Oxalobacteraceae"              
## bASV_214  "f__Gemmatimonadaceae"             
## bASV_215  "f__Intrasporangiaceae"            
## bASV_216  "f__Sphingobacteriaceae"           
## bASV_217  "f__Xanthomonadaceae"              
## bASV_218  "f__Comamonadaceae"                
## bASV_219  "f__Rhizobiaceae"                  
## bASV_220  "f__Comamonadaceae"                
## bASV_221  "f__Sphingobacteriaceae"           
## bASV_222  "f__Intrasporangiaceae"            
## bASV_223  NA                                 
## bASV_224  "f__Comamonadaceae"                
## bASV_225  "f__Spirosomaceae"                 
## bASV_226  "f__Xanthobacteraceae"             
## bASV_227  "f__Subgroup_7"                    
## bASV_228  "f__Xanthomonadaceae"              
## bASV_229  "f__Gemmatimonadaceae"             
## bASV_230  "f__Comamonadaceae"                
## bASV_231  "f__Sphingomonadaceae"             
## bASV_232  "f__Oxalobacteraceae"              
## bASV_233  "f__Chitinophagaceae"              
## bASV_234  "f__Blastocatellaceae"             
## bASV_235  "f__Xanthomonadaceae"              
## bASV_237  "f__SC-I-84"                       
## bASV_238  "f__Sphingomonadaceae"             
## bASV_240  "f__WD2101_soil_group"             
## bASV_241  "f__Pseudonocardiaceae"            
## bASV_242  "f__Chitinophagaceae"              
## bASV_243  "f__Comamonadaceae"                
## bASV_244  "f__Gemmatimonadaceae"             
## bASV_245  "f__Streptomycetaceae"             
## bASV_246  "f__Streptomycetaceae"             
## bASV_247  "f__Chitinophagaceae"              
## bASV_249  "f__Micromonosporaceae"            
## bASV_250  "f__Oxalobacteraceae"              
## bASV_251  "f__Sphingomonadaceae"             
## bASV_252  "f__Sphingomonadaceae"             
## bASV_253  "f__Oxalobacteraceae"              
## bASV_254  "f__Blastocatellaceae"             
## bASV_255  "f__Comamonadaceae"                
## bASV_256  "f__Sphingomonadaceae"             
## bASV_257  "f__Rhizobiaceae"                  
## bASV_258  "f__Oxalobacteraceae"              
## bASV_259  "f__Herpetosiphonaceae"            
## bASV_260  "f__SC-I-84"                       
## bASV_261  "f__uncultured"                    
## bASV_262  "f__Sphingomonadaceae"             
## bASV_263  "f__Microscillaceae"               
## bASV_264  "f__Comamonadaceae"                
## bASV_265  "f__Gemmatimonadaceae"             
## bASV_266  "f__Comamonadaceae"                
## bASV_268  "f__Chitinophagaceae"              
## bASV_269  "f__Oxalobacteraceae"              
## bASV_270  "f__Steroidobacteraceae"           
## bASV_271  "f__Reyranellaceae"                
## bASV_272  "f__Gemmatimonadaceae"             
## bASV_273  "f__Comamonadaceae"                
## bASV_274  "f__Oxalobacteraceae"              
## bASV_275  "f__Gitt-GS-136"                   
## bASV_276  "f__Oxalobacteraceae"              
## bASV_277  "f__Comamonadaceae"                
## bASV_278  "f__Comamonadaceae"                
## bASV_279  "f__Gemmatimonadaceae"             
## bASV_280  "f__Chitinophagaceae"              
## bASV_281  "f__Xanthobacteraceae"             
## bASV_282  "f__Caulobacteraceae"              
## bASV_283  "f__Caulobacteraceae"              
## bASV_285  "f__Oxalobacteraceae"              
## bASV_286  "f__Beijerinckiaceae"              
## bASV_288  "f__Rhodanobacteraceae"            
## bASV_289  "f__Oxalobacteraceae"              
## bASV_290  "f__Alcaligenaceae"                
## bASV_291  "f__Pseudonocardiaceae"            
## bASV_292  "f__Bacillaceae"                   
## bASV_293  "f__Oxalobacteraceae"              
## bASV_294  "f__Blastocatellaceae"             
## bASV_295  "f__Comamonadaceae"                
## bASV_297  "f__Caulobacteraceae"              
## bASV_298  "f__Xanthobacteraceae"             
## bASV_299  "f__Blastocatellaceae"             
## bASV_300  "f__Oxalobacteraceae"              
## bASV_302  "f__Oxalobacteraceae"              
## bASV_303  "f__Saccharimonadaceae"            
## bASV_304  "f__Nitrospiraceae"                
## bASV_305  "f__Xanthomonadaceae"              
## bASV_306  "f__Oxalobacteraceae"              
## bASV_307  "f__A21b"                          
## bASV_308  "f__Oxalobacteraceae"              
## bASV_309  "f__Comamonadaceae"                
## bASV_310  "f__Caulobacteraceae"              
## bASV_312  "f__Comamonadaceae"                
## bASV_313  "f__Rubritaleaceae"                
## bASV_314  "f__Comamonadaceae"                
## bASV_315  "f__Hyphomicrobiaceae"             
## bASV_316  "f__Gemmatimonadaceae"             
## bASV_317  "f__Sphingomonadaceae"             
## bASV_318  "f__Sphingomonadaceae"             
## bASV_319  "f__Unknown_Family"                
## bASV_321  "f__Sphingomonadaceae"             
## bASV_322  "f__Xanthomonadaceae"              
## bASV_323  "f__Sphingobacteriaceae"           
## bASV_324  "f__Spirosomaceae"                 
## bASV_325  "f__Burkholderiaceae"              
## bASV_326  "f__Blastocatellaceae"             
## bASV_327  "f__Xanthomonadaceae"              
## bASV_328  "f__Oxalobacteraceae"              
## bASV_330  "f__Chitinophagaceae"              
## bASV_331  "f__Rhodanobacteraceae"            
## bASV_332  "f__Gemmatimonadaceae"             
## bASV_333  "f__Spirosomaceae"                 
## bASV_334  "f__Sphingobacteriaceae"           
## bASV_335  "f__Nocardioidaceae"               
## bASV_336  "f__Oxalobacteraceae"              
## bASV_337  "f__Comamonadaceae"                
## bASV_339  "f__Xanthobacteraceae"             
## bASV_340  "f__Sphingomonadaceae"             
## bASV_341  "f__Comamonadaceae"                
## bASV_342  "f__Comamonadaceae"                
## bASV_343  "f__Oxalobacteraceae"              
## bASV_345  "f__Chitinophagaceae"              
## bASV_346  "f__SC-I-84"                       
## bASV_347  "f__Cellvibrionaceae"              
## bASV_348  "f__Sphingomonadaceae"             
## bASV_349  "f__Oxalobacteraceae"              
## bASV_350  "f__Sphingomonadaceae"             
## bASV_351  "f__Rhizobiaceae"                  
## bASV_352  "f__Sphingomonadaceae"             
## bASV_353  "f__Caulobacteraceae"              
## bASV_354  "f__Chitinophagaceae"              
## bASV_355  "f__Sphingomonadaceae"             
## bASV_356  "f__Comamonadaceae"                
## bASV_357  "f__Burkholderiaceae"              
## bASV_358  "f__Sphingobacteriaceae"           
## bASV_359  "f__Caulobacteraceae"              
## bASV_361  "f__Sphingomonadaceae"             
## bASV_362  "f__SC-I-84"                       
## bASV_363  "f__Microscillaceae"               
## bASV_364  "f__Methyloligellaceae"            
## bASV_365  "f__Chitinophagaceae"              
## bASV_366  "f__Oxalobacteraceae"              
## bASV_367  "f__Gemmatimonadaceae"             
## bASV_368  "f__Xanthobacteraceae"             
## bASV_369  "f__Comamonadaceae"                
## bASV_370  "f__Saccharimonadales"             
## bASV_371  "f__Rhodanobacteraceae"            
## bASV_372  "f__Comamonadaceae"                
## bASV_374  "f__Gemmatimonadaceae"             
## bASV_375  "f__Oxalobacteraceae"              
## bASV_376  "f__Comamonadaceae"                
## bASV_377  "f__Gitt-GS-136"                   
## bASV_378  "f__Gemmatimonadaceae"             
## bASV_379  "f__Burkholderiaceae"              
## bASV_380  "f__Myxococcaceae"                 
## bASV_381  "f__Xanthomonadaceae"              
## bASV_382  "f__SC-I-84"                       
## bASV_383  "f__Gemmatimonadaceae"             
## bASV_384  "f__Comamonadaceae"                
## bASV_385  "f__Nocardioidaceae"               
## bASV_386  "f__Sphingomonadaceae"             
## bASV_387  "f__Chitinophagaceae"              
## bASV_388  "f__Rhizobiaceae"                  
## bASV_389  "f__Nitrosomonadaceae"             
## bASV_390  "f__Oxalobacteraceae"              
## bASV_391  "f__Bryobacteraceae"               
## bASV_392  "f__Reyranellaceae"                
## bASV_395  "f__Gemmatimonadaceae"             
## bASV_396  "f__Oxalobacteraceae"              
## bASV_397  "f__Gemmatimonadaceae"             
## bASV_399  "f__Comamonadaceae"                
## bASV_400  "f__Nitrosomonadaceae"             
## bASV_401  "f__Xanthobacteraceae"             
## bASV_402  "f__Rhizobiaceae"                  
## bASV_403  "f__Burkholderiaceae"              
## bASV_404  "f__Comamonadaceae"                
## bASV_406  "f__Xanthomonadaceae"              
## bASV_407  "f__Fimbriimonadaceae"             
## bASV_408  "f__Blastocatellaceae"             
## bASV_409  "f__Nocardioidaceae"               
## bASV_410  "f__Burkholderiaceae"              
## bASV_411  "f__Rhodanobacteraceae"            
## bASV_412  "f__Comamonadaceae"                
## bASV_413  "f__Rhodanobacteraceae"            
## bASV_414  "f__Pseudomonadaceae"              
## bASV_416  "f__Nitrosomonadaceae"             
## bASV_417  "f__Beijerinckiaceae"              
## bASV_418  "f__Beijerinckiaceae"              
## bASV_419  "f__Oxalobacteraceae"              
## bASV_420  "f__Gaiellaceae"                   
## bASV_421  "f__Longimicrobiaceae"             
## bASV_422  "f__Sphingomonadaceae"             
## bASV_423  "f__Oxalobacteraceae"              
## bASV_424  "f__Xanthomonadaceae"              
## bASV_425  "f__Ilumatobacteraceae"            
## bASV_426  "f__Sphingomonadaceae"             
## bASV_427  "f__Vicinamibacteraceae"           
## bASV_428  "f__Unknown_Family"                
## bASV_429  "f__Chitinophagaceae"              
## bASV_430  "f__Caulobacteraceae"              
## bASV_431  "f__Hymenobacteraceae"             
## bASV_432  "f__Longimicrobiaceae"             
## bASV_433  "f__Steroidobacteraceae"           
## bASV_434  "f__Nocardioidaceae"               
## bASV_435  "f__Acidobacteriaceae_(Subgroup_1)"
## bASV_436  "f__Hymenobacteraceae"             
## bASV_438  "f__Beijerinckiaceae"              
## bASV_439  "f__Micropepsaceae"                
## bASV_440  "f__SC-I-84"                       
## bASV_441  "f__Oxalobacteraceae"              
## bASV_442  "f__Roseiflexaceae"                
## bASV_443  "f__Microscillaceae"               
## bASV_446  "f__Sphingomonadaceae"             
## bASV_447  "f__Blastocatellaceae"             
## bASV_448  "f__Chitinophagaceae"              
## bASV_449  "f__Beijerinckiaceae"              
## bASV_450  "f__Xanthobacteraceae"             
## bASV_451  "f__Dongiaceae"                    
## bASV_453  "f__Methylophilaceae"              
## bASV_454  "f__Geodermatophilaceae"           
## bASV_455  "f__Sphingobacteriaceae"           
## bASV_456  "f__Nocardioidaceae"               
## bASV_457  "f__Oxalobacteraceae"              
## bASV_458  "f__Nocardioidaceae"               
## bASV_459  "f__Sphingobacteriaceae"           
## bASV_460  "f__Nocardioidaceae"               
## bASV_461  "f__Sphingomonadaceae"             
## bASV_462  "f__Sphingomonadaceae"             
## bASV_464  "f__Gemmatimonadaceae"             
## bASV_465  "f__WD2101_soil_group"             
## bASV_466  "f__Pseudonocardiaceae"            
## bASV_467  "f__uncultured"                    
## bASV_468  "f__Gemmatimonadaceae"             
## bASV_469  "f__Nitrosomonadaceae"             
## bASV_471  "f__Oxalobacteraceae"              
## bASV_472  "f__Chitinophagaceae"              
## bASV_474  "f__Comamonadaceae"                
## bASV_476  "f__Comamonadaceae"                
## bASV_477  "f__Comamonadaceae"                
## bASV_478  "f__Oxalobacteraceae"              
## bASV_479  "f__Xanthomonadaceae"              
## bASV_480  "f__Thermomonosporaceae"           
## bASV_481  "f__Sandaracinaceae"               
## bASV_482  "f__Comamonadaceae"                
## bASV_483  "f__Intrasporangiaceae"            
## bASV_484  "f__Comamonadaceae"                
## bASV_485  "f__Oxalobacteraceae"              
## bASV_486  "f__Chitinophagaceae"              
## bASV_487  "f__Oxalobacteraceae"              
## bASV_488  "f__Rhodanobacteraceae"            
## bASV_489  "f__Mycobacteriaceae"              
## bASV_490  "f__Xanthomonadaceae"              
## bASV_491  "f__Bacillaceae"                   
## bASV_492  "f__uncultured"                    
## bASV_493  "f__Flavobacteriaceae"             
## bASV_494  "f__Chitinophagaceae"              
## bASV_495  "f__Comamonadaceae"                
## bASV_496  "f__Unknown_Family"                
## bASV_498  "f__Pyrinomonadaceae"              
## bASV_499  "f__Sphingomonadaceae"             
## bASV_500  "f__uncultured"                    
## bASV_501  "f__Solibacteraceae"               
## bASV_503  "f__Blastocatellaceae"             
## bASV_504  "f__Xanthomonadaceae"              
## bASV_505  "f__Nocardioidaceae"               
## bASV_506  "f__Sphingomonadaceae"             
## bASV_507  "f__Comamonadaceae"                
## bASV_508  "f__Dongiaceae"                    
## bASV_509  "f__Dongiaceae"                    
## bASV_510  "f__Sphingomonadaceae"             
## bASV_511  "f__Methylophilaceae"              
## bASV_512  "f__Cellvibrionaceae"              
## bASV_513  "f__Blastocatellaceae"             
## bASV_514  "f__Comamonadaceae"                
## bASV_515  "f__Caulobacteraceae"              
## bASV_516  "f__Reyranellaceae"                
## bASV_517  "f__Flavobacteriaceae"             
## bASV_518  "f__Comamonadaceae"                
## bASV_519  "f__Bryobacteraceae"               
## bASV_520  "f__Gemmatimonadaceae"             
## bASV_521  "f__Comamonadaceae"                
## bASV_522  "f__Chitinophagaceae"              
## bASV_524  "f__Sphingomonadaceae"             
## bASV_525  "f__Burkholderiaceae"              
## bASV_526  "f__Xanthobacteraceae"             
## bASV_527  "f__IMCC26256"                     
## bASV_528  "f__Comamonadaceae"                
## bASV_529  "f__Oxalobacteraceae"              
## bASV_530  "f__IMCC26256"                     
## bASV_531  "f__Gemmatimonadaceae"             
## bASV_533  "f__Weeksellaceae"                 
## bASV_534  "f__Blastocatellaceae"             
## bASV_535  "f__Flavobacteriaceae"             
## bASV_536  "f__Nocardioidaceae"               
## bASV_537  "f__Comamonadaceae"                
## bASV_539  "f__Comamonadaceae"                
## bASV_540  "f__Oxalobacteraceae"              
## bASV_541  "f__Sphingomonadaceae"             
## bASV_542  "f__Glycomycetaceae"               
## bASV_546  "f__uncultured"                    
## bASV_547  "f__Sphingobacteriaceae"           
## bASV_548  "f__Xanthomonadaceae"              
## bASV_549  "f__Gemmatimonadaceae"             
## bASV_550  "f__Oxalobacteraceae"              
## bASV_551  "f__Pyrinomonadaceae"              
## bASV_552  "f__Rhodanobacteraceae"            
## bASV_553  "f__Sphingobacteriaceae"           
## bASV_554  "f__Streptosporangiaceae"          
## bASV_556  "f__Nocardioidaceae"               
## bASV_557  "f__Xanthomonadaceae"              
## bASV_558  "f__Chitinophagaceae"              
## bASV_561  "f__Rhodanobacteraceae"            
## bASV_562  "f__Caulobacteraceae"              
## bASV_563  "f__Comamonadaceae"                
## bASV_564  "f__Chitinophagaceae"              
## bASV_565  "f__Sphingomonadaceae"             
## bASV_567  "f__Caulobacteraceae"              
## bASV_569  "f__Flavobacteriaceae"             
## bASV_570  "f__Chitinophagaceae"              
## bASV_571  "f__IMCC26256"                     
## bASV_572  "f__Sphingomonadaceae"             
## bASV_573  "f__Labraceae"                     
## bASV_574  "f__uncultured"                    
## bASV_575  "f__WD2101_soil_group"             
## bASV_576  "f__uncultured"                    
## bASV_577  "f__Gemmatimonadaceae"             
## bASV_578  "f__Solibacteraceae"               
## bASV_579  "f__KD4-96"                        
## bASV_580  "f__SC-I-84"                       
## bASV_581  "f__Rhizobiaceae"                  
## bASV_582  "f__Rokubacteriales"               
## bASV_583  "f__Solibacteraceae"               
## bASV_584  "f__Caulobacteraceae"              
## bASV_585  "f__Vicinamibacteraceae"           
## bASV_586  "f__WD2101_soil_group"             
## bASV_587  "f__Comamonadaceae"                
## bASV_590  "f__Oxalobacteraceae"              
## bASV_591  "f__Micropepsaceae"                
## bASV_592  "f__Burkholderiaceae"              
## bASV_593  "f__Pseudonocardiaceae"            
## bASV_595  "f__Gemmatimonadaceae"             
## bASV_596  "f__Rhodanobacteraceae"            
## bASV_597  "f__Unknown_Family"                
## bASV_598  "f__Intrasporangiaceae"            
## bASV_599  "f__Hyphomicrobiaceae"             
## bASV_600  "f__Sphingobacteriaceae"           
## bASV_601  "f__WD2101_soil_group"             
## bASV_602  "f__Rhizobiaceae"                  
## bASV_603  "f__Oxalobacteraceae"              
## bASV_604  "f__WD2101_soil_group"             
## bASV_605  "f__Xanthomonadaceae"              
## bASV_606  "f__Comamonadaceae"                
## bASV_607  "f__Gemmatimonadaceae"             
## bASV_609  "f__Subgroup_7"                    
## bASV_611  "f__Sphingomonadaceae"             
## bASV_612  "f__Vicinamibacteraceae"           
## bASV_613  "f__Gemmatimonadaceae"             
## bASV_614  "f__Nocardioidaceae"               
## bASV_615  "f__Oxalobacteraceae"              
## bASV_616  "f__Gaiellaceae"                   
## bASV_617  "f__Rhizobiaceae"                  
## bASV_619  "f__SC-I-84"                       
## bASV_621  "f__Hyphomicrobiaceae"             
## bASV_622  "f__Subgroup_7"                    
## bASV_623  "f__Oxalobacteraceae"              
## bASV_624  "f__Sphingomonadaceae"             
## bASV_625  "f__Oxalobacteraceae"              
## bASV_626  "f__Oxalobacteraceae"              
## bASV_627  "f__Oxalobacteraceae"              
## bASV_628  "f__Chitinophagaceae"              
## bASV_629  "f__uncultured"                    
## bASV_632  "f__Micropepsaceae"                
## bASV_635  "f__Sphingomonadaceae"             
## bASV_636  "f__Xanthomonadaceae"              
## bASV_637  "f__Gemmatimonadaceae"             
## bASV_638  "f__Haliangiaceae"                 
## bASV_639  "f__Sphingomonadaceae"             
## bASV_640  "f__Oxalobacteraceae"              
## bASV_641  "f__Xanthobacteraceae"             
## bASV_642  "f__Intrasporangiaceae"            
## bASV_643  "f__Pyrinomonadaceae"              
## bASV_645  "f__Caulobacteraceae"              
## bASV_646  "f__Oxalobacteraceae"              
## bASV_647  "f__Sphingomonadaceae"             
## bASV_648  "f__Pyrinomonadaceae"              
## bASV_649  "f__Chthoniobacteraceae"           
## bASV_650  "f__Comamonadaceae"                
## bASV_651  "f__Sphingobacteriaceae"           
## bASV_652  "f__Xanthomonadaceae"              
## bASV_653  "f__Gemmatimonadaceae"             
## bASV_654  "f__Dongiaceae"                    
## bASV_655  "f__Flavobacteriaceae"             
## bASV_657  "f__Oxalobacteraceae"              
## bASV_658  "f__Micrococcaceae"                
## bASV_659  "f__67-14"                         
## bASV_660  "f__uncultured"                    
## bASV_661  "f__IMCC26256"                     
## bASV_662  "f__SC-I-84"                       
## bASV_663  "f__Oxalobacteraceae"              
## bASV_664  "f__Methyloligellaceae"            
## bASV_665  "f__Caulobacteraceae"              
## bASV_666  "f__Gemmatimonadaceae"             
## bASV_667  "f__MB-A2-108"                     
## bASV_668  "f__PLTA13"                        
## bASV_669  "f__Comamonadaceae"                
## bASV_670  "f__Subgroup_17"                   
## bASV_672  "f__Comamonadaceae"                
## bASV_674  "f__WD2101_soil_group"             
## bASV_675  "f__uncultured"                    
## bASV_676  "f__Blastocatellaceae"             
## bASV_677  "f__Oxalobacteraceae"              
## bASV_678  "f__Sphingomonadaceae"             
## bASV_682  "f__Caulobacteraceae"              
## bASV_683  "f__Glycomycetaceae"               
## bASV_684  "f__Paenibacillaceae"              
## bASV_687  "f__uncultured"                    
## bASV_688  "f__Unknown_Family"                
## bASV_691  "f__Subgroup_2"                    
## bASV_692  "f__AD3"                           
## bASV_693  "f__uncultured"                    
## bASV_694  "f__Comamonadaceae"                
## bASV_695  "f__Nocardioidaceae"               
## bASV_696  "f__Steroidobacteraceae"           
## bASV_698  "f__Rhizobiales_Incertae_Sedis"    
## bASV_699  "f__KD4-96"                        
## bASV_700  "f__Gemmatimonadaceae"             
## bASV_701  "f__Comamonadaceae"                
## bASV_702  "f__uncultured"                    
## bASV_703  "f__Comamonadaceae"                
## bASV_704  "f__Gemmatimonadaceae"             
## bASV_705  "f__Nocardioidaceae"               
## bASV_706  "f__Chitinophagaceae"              
## bASV_707  "f__Vicinamibacteraceae"           
## bASV_708  "f__Sphingobacteriaceae"           
## bASV_709  "f__Unknown_Family"                
## bASV_711  "f__Nocardioidaceae"               
## bASV_712  "f__Burkholderiaceae"              
## bASV_713  "f__uncultured"                    
## bASV_714  "f__Roseiflexaceae"                
## bASV_715  "f__Pseudonocardiaceae"            
## bASV_716  "f__SC-I-84"                       
## bASV_717  "f__Chitinophagaceae"              
## bASV_719  "f__Paenibacillaceae"              
## bASV_720  "f__Gemmatimonadaceae"             
## bASV_721  "f__Chitinophagaceae"              
## bASV_722  "f__Comamonadaceae"                
## bASV_724  "f__Flavobacteriaceae"             
## bASV_725  "f__Chthoniobacteraceae"           
## bASV_726  "f__IMCC26256"                     
## bASV_728  "f__Sphingomonadaceae"             
## bASV_729  "f__Xanthomonadaceae"              
## bASV_730  "f__Oxalobacteraceae"              
## bASV_731  "f__Oxalobacteraceae"              
## bASV_732  "f__Chthoniobacteraceae"           
## bASV_733  "f__Chthoniobacteraceae"           
## bASV_735  "f__Fimbriimonadaceae"             
## bASV_736  "f__Nitrospiraceae"                
## bASV_737  "f__uncultured"                    
## bASV_738  "f__Xanthobacteraceae"             
## bASV_739  "f__Paenibacillaceae"              
## bASV_740  "f__Gemmatimonadaceae"             
## bASV_741  "f__uncultured"                    
## bASV_743  "f__Chitinophagaceae"              
## bASV_744  "f__Comamonadaceae"                
## bASV_745  "f__Comamonadaceae"                
## bASV_746  "f__Rhizobiaceae"                  
## bASV_747  "f__Gemmatimonadaceae"             
## bASV_749  "f__Hyphomicrobiaceae"             
## bASV_752  "f__Sphingomonadaceae"             
## bASV_753  "f__Vicinamibacteraceae"           
## bASV_754  "f__Oxalobacteraceae"              
## bASV_755  "f__Oxalobacteraceae"              
## bASV_756  "f__Oxalobacteraceae"              
## bASV_757  "f__Caulobacteraceae"              
## bASV_758  "f__BIrii41"                       
## bASV_759  "f__Hyphomicrobiaceae"             
## bASV_760  "f__Sphingomonadaceae"             
## bASV_761  "f__uncultured"                    
## bASV_762  "f__Subgroup_7"                    
## bASV_763  "f__WD2101_soil_group"             
## bASV_764  "f__Gemmatimonadaceae"             
## bASV_766  "f__Sphingomonadaceae"             
## bASV_767  "f__Opitutaceae"                   
## bASV_769  "f__Subgroup_7"                    
## bASV_770  "f__Polyangiaceae"                 
## bASV_771  "f__Intrasporangiaceae"            
## bASV_772  "f__Comamonadaceae"                
## bASV_773  "f__Xanthobacteraceae"             
## bASV_776  "f__mle1-27"                       
## bASV_777  "f__Nitrospiraceae"                
## bASV_780  "f__Oxalobacteraceae"              
## bASV_782  "f__Nocardioidaceae"               
## bASV_784  "f__Rhodanobacteraceae"            
## bASV_785  "f__Oxalobacteraceae"              
## bASV_786  "f__Comamonadaceae"                
## bASV_787  "f__Chitinophagaceae"              
## bASV_788  "f__Pseudonocardiaceae"            
## bASV_789  "f__Alcaligenaceae"                
## bASV_791  "f__Pedosphaeraceae"               
## bASV_792  "f__Comamonadaceae"                
## bASV_793  "f__Chitinophagaceae"              
## bASV_794  "f__Nitrosomonadaceae"             
## bASV_795  "f__Gemmatimonadaceae"             
## bASV_797  "f__Streptomycetaceae"             
## bASV_798  "f__Sphingomonadaceae"             
## bASV_799  "f__C0119"                         
## bASV_800  "f__Gemmatimonadaceae"             
## bASV_801  "f__Comamonadaceae"                
## bASV_803  "f__Chitinophagaceae"              
## bASV_804  "f__Oxalobacteraceae"              
## bASV_805  "f__Oxalobacteraceae"              
## bASV_806  "f__Chitinophagaceae"              
## bASV_807  "f__Pyrinomonadaceae"              
## bASV_808  "f__KD4-96"                        
## bASV_809  "f__Bacillaceae"                   
## bASV_810  "f__Polyangiaceae"                 
## bASV_812  "f__Chitinophagaceae"              
## bASV_813  "f__Sphingomonadaceae"             
## bASV_814  "f__Xanthobacteraceae"             
## bASV_816  "f__Unknown_Family"                
## bASV_817  "f__Burkholderiaceae"              
## bASV_819  "f__Nocardioidaceae"               
## bASV_820  "f__Nitrosomonadaceae"             
## bASV_821  "f__Gemmatimonadaceae"             
## bASV_822  "f__Acidobacteriaceae_(Subgroup_1)"
## bASV_823  "f__Gemmatimonadaceae"             
## bASV_824  "f__Nocardioidaceae"               
## bASV_825  "f__Oxalobacteraceae"              
## bASV_826  "f__A21b"                          
## bASV_827  "f__Xanthomonadaceae"              
## bASV_828  "f__Oxalobacteraceae"              
## bASV_829  "f__Micropepsaceae"                
## bASV_830  "f__Subgroup_17"                   
## bASV_831  "f__Comamonadaceae"                
## bASV_832  "f__Xanthomonadaceae"              
## bASV_833  "f__Chthoniobacteraceae"           
## bASV_835  "f__Sphingomonadaceae"             
## bASV_836  "f__Oxalobacteraceae"              
## bASV_837  "f__Pyrinomonadaceae"              
## bASV_838  "f__Xanthomonadaceae"              
## bASV_840  "f__uncultured"                    
## bASV_841  "f__Oxalobacteraceae"              
## bASV_842  "f__Hymenobacteraceae"             
## bASV_843  "f__Oxalobacteraceae"              
## bASV_844  "f__Nitrosomonadaceae"             
## bASV_845  "f__Comamonadaceae"                
## bASV_846  NA                                 
## bASV_847  "f__Oxalobacteraceae"              
## bASV_848  "f__Microscillaceae"               
## bASV_849  "f__uncultured"                    
## bASV_851  "f__Weeksellaceae"                 
## bASV_854  "f__Oxalobacteraceae"              
## bASV_855  "f__Beijerinckiaceae"              
## bASV_856  "f__uncultured"                    
## bASV_857  "f__Nitrosomonadaceae"             
## bASV_858  "f__Chitinophagaceae"              
## bASV_859  "f__Caulobacteraceae"              
## bASV_860  "f__SC-I-84"                       
## bASV_861  "f__Blastocatellaceae"             
## bASV_865  "f__Gemmatimonadaceae"             
## bASV_866  "f__Bacillaceae"                   
## bASV_867  "f__WD2101_soil_group"             
## bASV_868  "f__SC-I-84"                       
## bASV_869  "f__Pyrinomonadaceae"              
## bASV_870  "f__Rhodocyclaceae"                
## bASV_871  "f__Paenibacillaceae"              
## bASV_874  "f__Opitutaceae"                   
## bASV_875  "f__Xanthomonadaceae"              
## bASV_876  "f__Oxalobacteraceae"              
## bASV_877  "f__uncultured"                    
## bASV_878  "f__SC-I-84"                       
## bASV_879  "f__Comamonadaceae"                
## bASV_880  "f__SC-I-84"                       
## bASV_882  "f__Nitrosomonadaceae"             
## bASV_883  "f__Methylophilaceae"              
## bASV_885  "f__Xanthobacteraceae"             
## bASV_886  "f__Deinococcaceae"                
## bASV_887  "f__Gemmatimonadaceae"             
## bASV_888  "f__Comamonadaceae"                
## bASV_890  "f__uncultured"                    
## bASV_891  "f__Myxococcaceae"                 
## bASV_892  "f__Chitinophagaceae"              
## bASV_893  "f__A21b"                          
## bASV_895  "f__Gemmatimonadaceae"             
## bASV_896  "f__11-24"                         
## bASV_897  "f__uncultured"                    
## bASV_898  "f__uncultured"                    
## bASV_901  "f__Chitinophagaceae"              
## bASV_902  "f__uncultured"                    
## bASV_903  "f__Gemmataceae"                   
## bASV_904  "f__Flavobacteriaceae"             
## bASV_905  "f__Pseudonocardiaceae"            
## bASV_906  "f__Gemmatimonadaceae"             
## bASV_907  "f__Pseudomonadaceae"              
## bASV_908  "f__Caulobacteraceae"              
## bASV_909  "f__Oxalobacteraceae"              
## bASV_910  "f__Micropepsaceae"                
## bASV_911  "f__Comamonadaceae"                
## bASV_912  "f__Xanthobacteraceae"             
## bASV_914  "f__Oxalobacteraceae"              
## bASV_915  "f__Comamonadaceae"                
## bASV_916  NA                                 
## bASV_917  "f__uncultured"                    
## bASV_918  "f__Flavobacteriaceae"             
## bASV_920  "f__Gemmatimonadaceae"             
## bASV_921  "f__Comamonadaceae"                
## bASV_922  "f__Xanthobacteraceae"             
## bASV_923  "f__Micromonosporaceae"            
## bASV_924  "f__WD2101_soil_group"             
## bASV_925  "f__Comamonadaceae"                
## bASV_926  "f__Methyloligellaceae"            
## bASV_928  "f__Steroidobacteraceae"           
## bASV_930  "f__Xanthomonadaceae"              
## bASV_931  "f__Solibacteraceae"               
## bASV_933  "f__Sphingomonadaceae"             
## bASV_934  "f__Xanthobacteraceae"             
## bASV_935  "f__Chthoniobacteraceae"           
## bASV_936  "f__Tepidisphaeraceae"             
## bASV_937  "f__Crocinitomicaceae"             
## bASV_939  "f__Xanthobacteraceae"             
## bASV_940  "f__Xanthobacteraceae"             
## bASV_941  "f__SC-I-84"                       
## bASV_945  "f__Sphingomonadaceae"             
## bASV_946  "f__Comamonadaceae"                
## bASV_947  "f__Oxalobacteraceae"              
## bASV_948  "f__Oxalobacteraceae"              
## bASV_949  "f__Alcaligenaceae"                
## bASV_950  "f__Bryobacteraceae"               
## bASV_951  "f__Rhizobiales_Incertae_Sedis"    
## bASV_952  "f__Spirosomaceae"                 
## bASV_953  "f__Saccharimonadaceae"            
## bASV_954  "f__Nitrosomonadaceae"             
## bASV_955  "f__Rhizobiales_Incertae_Sedis"    
## bASV_956  "f__Microscillaceae"               
## bASV_958  "f__Ilumatobacteraceae"            
## bASV_959  "f__IMCC26256"                     
## bASV_960  "f__SC-I-84"                       
## bASV_961  "f__Solibacteraceae"               
## bASV_963  "f__Gemmatimonadaceae"             
## bASV_964  "f__BIrii41"                       
## bASV_966  "f__C0119"                         
## bASV_969  "f__uncultured"                    
## bASV_972  "f__Caulobacteraceae"              
## bASV_973  "f__WD2101_soil_group"             
## bASV_974  "f__Micropepsaceae"                
## bASV_975  "f__Chitinophagaceae"              
## bASV_976  "f__Nitrosomonadaceae"             
## bASV_977  "f__Subgroup_7"                    
## bASV_979  "f__Pedosphaeraceae"               
## bASV_980  "f__Comamonadaceae"                
## bASV_981  "f__Vicinamibacteraceae"           
## bASV_982  "f__Xanthomonadaceae"              
## bASV_983  "f__Pyrinomonadaceae"              
## bASV_984  "f__Rhizobiales_Incertae_Sedis"    
## bASV_986  "f__Xanthomonadaceae"              
## bASV_988  "f__Solibacteraceae"               
## bASV_989  "f__Comamonadaceae"                
## bASV_990  "f__Beijerinckiaceae"              
## bASV_991  "f__Gemmatimonadaceae"             
## bASV_992  "f__uncultured"                    
## bASV_994  "f__Chitinophagaceae"              
## bASV_995  "f__uncultured"                    
## bASV_996  "f__Xanthomonadaceae"              
## bASV_998  "f__uncultured"                    
## bASV_999  "f__Oxalobacteraceae"              
## bASV_1001 "f__Xanthobacteraceae"             
## bASV_1003 "f__Gemmatimonadaceae"             
## bASV_1005 "f__Gemmatimonadaceae"             
## bASV_1006 "f__Rokubacteriales"               
## bASV_1007 "f__Blastocatellaceae"             
## bASV_1008 "f__Rhizobiaceae"                  
## bASV_1010 "f__Gemmatimonadaceae"             
## bASV_1011 "f__Pseudonocardiaceae"            
## bASV_1012 "f__Iamiaceae"                     
## bASV_1013 "f__uncultured"                    
## bASV_1014 "f__Rhodanobacteraceae"            
## bASV_1015 "f__Bacillaceae"                   
## bASV_1016 "f__Gemmatimonadaceae"             
## bASV_1017 "f__Chitinophagaceae"              
## bASV_1018 "f__Gemmatimonadaceae"             
## bASV_1019 "f__Rubritaleaceae"                
## bASV_1020 "f__WD2101_soil_group"             
## bASV_1021 "f__A21b"                          
## bASV_1022 "f__Vicinamibacteraceae"           
## bASV_1027 "f__Xanthomonadaceae"              
## bASV_1028 "f__WD2101_soil_group"             
## bASV_1029 "f__Comamonadaceae"                
## bASV_1030 "f__Rhodanobacteraceae"            
## bASV_1031 "f__Geodermatophilaceae"           
## bASV_1032 "f__Comamonadaceae"                
## bASV_1033 "f__Micropepsaceae"                
## bASV_1034 "f__Saccharimonadales"             
## bASV_1035 "f__Sphingobacteriaceae"           
## bASV_1037 "f__Chitinophagaceae"              
## bASV_1038 "f__Paenibacillaceae"              
## bASV_1039 "f__Rubritaleaceae"                
## bASV_1041 "f__uncultured"                    
## bASV_1042 "f__Comamonadaceae"                
## bASV_1045 NA                                 
## bASV_1046 "f__Rhodanobacteraceae"            
## bASV_1047 "f__Nitrosomonadaceae"             
## bASV_1048 "f__Oxalobacteraceae"              
## bASV_1049 "f__WD2101_soil_group"             
## bASV_1052 "f__JG30-KF-AS9"                   
## bASV_1054 "f__Crocinitomicaceae"             
## bASV_1055 "f__Blfdi19"                       
## bASV_1056 "f__Nocardioidaceae"               
## bASV_1057 "f__Chitinophagaceae"              
## bASV_1058 "f__Xanthobacteraceae"             
## bASV_1059 "f__Solibacteraceae"               
## bASV_1060 "f__Saccharimonadaceae"            
## bASV_1061 "f__Xanthobacteraceae"             
## bASV_1062 NA                                 
## bASV_1064 "f__SC-I-84"                       
## bASV_1066 "f__Micropepsaceae"                
## bASV_1067 "f__Dongiaceae"                    
## bASV_1068 "f__Glycomycetaceae"               
## bASV_1070 "f__Nitrosomonadaceae"             
## bASV_1071 "f__Hyphomonadaceae"               
## bASV_1072 "f__Oxalobacteraceae"              
## bASV_1073 "f__Oxalobacteraceae"              
## bASV_1074 "f__Nocardioidaceae"               
## bASV_1075 "f__Solibacteraceae"               
## bASV_1077 "f__Oxalobacteraceae"              
## bASV_1078 "f__uncultured"                    
## bASV_1079 "f__BIrii41"                       
## bASV_1080 "f__Pseudomonadaceae"              
## bASV_1081 "f__Subgroup_7"                    
## bASV_1082 "f__uncultured"                    
## bASV_1083 "f__Haliangiaceae"                 
## bASV_1084 "f__Xanthomonadaceae"              
## bASV_1085 "f__Vampirovibrionaceae"           
## bASV_1086 "f__Steroidobacteraceae"           
## bASV_1087 "f__Gemmatimonadaceae"             
## bASV_1088 "f__Intrasporangiaceae"            
## bASV_1089 "f__Roseiflexaceae"                
## bASV_1090 "f__Comamonadaceae"                
## bASV_1091 "f__Gemmatimonadaceae"             
## bASV_1092 "f__Nitrospiraceae"                
## bASV_1094 "f__Rubritaleaceae"                
## bASV_1095 "f__Acetobacteraceae"              
## bASV_1096 "f__Rhodanobacteraceae"            
## bASV_1098 "f__Chitinophagaceae"              
## bASV_1099 "f__Gemmatimonadaceae"             
## bASV_1102 "f__Burkholderiaceae"              
## bASV_1103 "f__Comamonadaceae"                
## bASV_1105 "f__Tepidisphaeraceae"             
## bASV_1107 "f__Hymenobacteraceae"             
## bASV_1108 "f__Opitutaceae"                   
## bASV_1109 "f__Comamonadaceae"                
## bASV_1110 "f__Sphingobacteriaceae"           
## bASV_1111 "f__SC-I-84"                       
## bASV_1112 "f__Pseudonocardiaceae"            
## bASV_1114 "f__Xanthobacteraceae"             
## bASV_1115 "f__Bryobacteraceae"               
## bASV_1116 "f__Sphingomonadaceae"             
## bASV_1117 "f__Bdellovibrionaceae"            
## bASV_1118 "f__Caulobacteraceae"              
## bASV_1119 "f__Sphingomonadaceae"             
## bASV_1120 NA                                 
## bASV_1121 "f__Subgroup_7"                    
## bASV_1122 "f__C0119"                         
## bASV_1123 "f__Beijerinckiaceae"              
## bASV_1124 "f__Xanthobacteraceae"             
## bASV_1125 "f__WD2101_soil_group"             
## bASV_1126 "f__Oxalobacteraceae"              
## bASV_1127 "f__Vicinamibacteraceae"           
## bASV_1128 "f__KF-JG30-B3"                    
## bASV_1129 "f__SC-I-84"                       
## bASV_1130 "f__uncultured"                    
## bASV_1131 "f__Unknown_Family"                
## bASV_1132 "f__uncultured"                    
## bASV_1133 "f__Comamonadaceae"                
## bASV_1134 "f__Polyangiaceae"                 
## bASV_1136 "f__Blastocatellaceae"             
## bASV_1137 "f__Alcaligenaceae"                
##           Genus                                                   Species
## bASV_5    "g__Acidovorax"                                         "s__"  
## bASV_7    "g__Pseudomonas"                                        "s__"  
## bASV_9    "g__Niastella"                                          "s__"  
## bASV_11   "g__Sphingomonas"                                       "s__"  
## bASV_12   "g__Streptomyces"                                       "s__"  
## bASV_13   "g__Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium" "s__"  
## bASV_14   "g__Rhizobacter"                                        "s__"  
## bASV_15   NA                                                      NA     
## bASV_16   "g__Massilia"                                           "s__"  
## bASV_18   NA                                                      NA     
## bASV_19   "g__Caulobacter"                                        "s__"  
## bASV_20   "g__Roseateles"                                         "s__"  
## bASV_21   "g__Sphingomonas"                                       "s__"  
## bASV_23   "g__Niastella"                                          "s__"  
## bASV_25   "g__Massilia"                                           "s__"  
## bASV_27   "g__Acidovorax"                                         "s__"  
## bASV_29   "g__Devosia"                                            "s__"  
## bASV_30   "g__Caulobacter"                                        "s__"  
## bASV_31   NA                                                      NA     
## bASV_32   "g__Massilia"                                           "s__"  
## bASV_33   "g__Pseudomonas"                                        "s__"  
## bASV_34   "g__Acidovorax"                                         "s__"  
## bASV_35   "g__Massilia"                                           "s__"  
## bASV_36   "g__Niastella"                                          "s__"  
## bASV_37   NA                                                      NA     
## bASV_38   NA                                                      NA     
## bASV_39   "g__Niastella"                                          "s__"  
## bASV_40   "g__Massilia"                                           "s__"  
## bASV_41   "g__Streptomyces"                                       "s__"  
## bASV_43   NA                                                      NA     
## bASV_44   NA                                                      NA     
## bASV_45   "g__Niastella"                                          "s__"  
## bASV_46   NA                                                      NA     
## bASV_48   "g__Bradyrhizobium"                                     "s__"  
## bASV_49   NA                                                      NA     
## bASV_50   NA                                                      NA     
## bASV_51   "g__Massilia"                                           "s__"  
## bASV_52   "g__Streptomyces"                                       "s__"  
## bASV_53   "g__Mesorhizobium"                                      "s__"  
## bASV_54   NA                                                      NA     
## bASV_55   "g__Streptomyces"                                       "s__"  
## bASV_56   "g__Massilia"                                           "s__"  
## bASV_57   NA                                                      NA     
## bASV_58   NA                                                      NA     
## bASV_59   NA                                                      NA     
## bASV_60   "g__Porphyrobacter"                                     "s__"  
## bASV_61   "g__Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium" "s__"  
## bASV_62   "g__Sphingomonas"                                       "s__"  
## bASV_63   NA                                                      NA     
## bASV_64   "g__Chryseolinea"                                       "s__"  
## bASV_65   "g__Massilia"                                           "s__"  
## bASV_66   "g__Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium" "s__"  
## bASV_67   "g__Bosea"                                              "s__"  
## bASV_68   NA                                                      NA     
## bASV_69   "g__Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium" "s__"  
## bASV_70   "g__Niastella"                                          "s__"  
## bASV_71   "g__Dyella"                                             "s__"  
## bASV_72   "g__Rhizobacter"                                        "s__"  
## bASV_73   "g__Phenylobacterium"                                   "s__"  
## bASV_74   "g__Niastella"                                          "s__"  
## bASV_75   "g__Massilia"                                           "s__"  
## bASV_76   "g__Asticcacaulis"                                      "s__"  
## bASV_77   "g__Rhizobacter"                                        "s__"  
## bASV_78   NA                                                      NA     
## bASV_79   "g__Massilia"                                           "s__"  
## bASV_80   "g__Luteimonas"                                         "s__"  
## bASV_81   NA                                                      NA     
## bASV_82   "g__Streptomyces"                                       "s__"  
## bASV_83   "g__Massilia"                                           "s__"  
## bASV_84   "g__Methylotenera"                                      "s__"  
## bASV_85   NA                                                      NA     
## bASV_86   "g__Massilia"                                           "s__"  
## bASV_87   "g__uncultured"                                         "s__"  
## bASV_88   NA                                                      NA     
## bASV_89   "g__Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium" "s__"  
## bASV_90   "g__Dyella"                                             "s__"  
## bASV_91   "g__Lysobacter"                                         "s__"  
## bASV_92   "g__Massilia"                                           "s__"  
## bASV_93   NA                                                      NA     
## bASV_94   "g__Massilia"                                           "s__"  
## bASV_95   "g__Rubrivivax"                                         "s__"  
## bASV_96   NA                                                      NA     
## bASV_97   "g__Pelomonas"                                          "s__"  
## bASV_98   "g__uncultured"                                         "s__"  
## bASV_99   "g__Dokdonella"                                         "s__"  
## bASV_100  "g__Phenylobacterium"                                   "s__"  
## bASV_101  "g__Pseudomonas"                                        "s__"  
## bASV_102  "g__Niastella"                                          "s__"  
## bASV_103  "g__Phenylobacterium"                                   "s__"  
## bASV_104  "g__Oxalicibacterium"                                   "s__"  
## bASV_105  "g__Pelomonas"                                          "s__"  
## bASV_106  "g__Lysobacter"                                         "s__"  
## bASV_107  "g__Parablastomonas"                                    "s__"  
## bASV_108  "g__Niastella"                                          "s__"  
## bASV_109  "g__Niastella"                                          "s__"  
## bASV_110  "g__Ellin6055"                                          "s__"  
## bASV_111  "g__Neorhizobium"                                       "s__"  
## bASV_112  "g__Pseudoduganella"                                    "s__"  
## bASV_113  NA                                                      NA     
## bASV_114  "g__Sphingomonas"                                       "s__"  
## bASV_115  "g__Brevundimonas"                                      "s__"  
## bASV_116  "g__Lysobacter"                                         "s__"  
## bASV_117  "g__Pseudoduganella"                                    "s__"  
## bASV_118  "g__uncultured"                                         "s__"  
## bASV_119  "g__Massilia"                                           "s__"  
## bASV_120  "g__Gemmatimonas"                                       "s__"  
## bASV_121  "g__Amycolatopsis"                                      "s__"  
## bASV_122  "g__Luteimonas"                                         "s__"  
## bASV_123  "g__Niastella"                                          "s__"  
## bASV_124  "g__Aeromicrobium"                                      "s__"  
## bASV_125  "g__uncultured"                                         "s__"  
## bASV_126  NA                                                      NA     
## bASV_128  "g__Nannocystis"                                        "s__"  
## bASV_129  NA                                                      NA     
## bASV_131  "g__Comamonas"                                          "s__"  
## bASV_132  "g__Lysobacter"                                         "s__"  
## bASV_133  "g__Pseudolabrys"                                       "s__"  
## bASV_134  NA                                                      NA     
## bASV_135  "g__Sphingomonas"                                       "s__"  
## bASV_136  "g__Massilia"                                           "s__"  
## bASV_137  "g__Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium" "s__"  
## bASV_138  "g__Bryobacter"                                         "s__"  
## bASV_139  "g__Burkholderia-Caballeronia-Paraburkholderia"         "s__"  
## bASV_140  NA                                                      NA     
## bASV_141  "g__Brevundimonas"                                      "s__"  
## bASV_142  "g__Lysobacter"                                         "s__"  
## bASV_143  "g__Ideonella"                                          "s__"  
## bASV_144  "g__Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium" "s__"  
## bASV_145  "g__Massilia"                                           "s__"  
## bASV_146  "g__Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium" "s__"  
## bASV_147  "g__Novosphingobium"                                    "s__"  
## bASV_148  "g__Burkholderia-Caballeronia-Paraburkholderia"         "s__"  
## bASV_149  NA                                                      NA     
## bASV_151  "g__Noviherbaspirillum"                                 "s__"  
## bASV_152  "g__Sphingomonas"                                       "s__"  
## bASV_153  "g__Massilia"                                           "s__"  
## bASV_154  "g__Fluviicola"                                         "s__"  
## bASV_155  NA                                                      NA     
## bASV_156  "g__Massilia"                                           "s__"  
## bASV_158  NA                                                      NA     
## bASV_159  "g__Pedobacter"                                         "s__"  
## bASV_160  "g__Sphingomonas"                                       "s__"  
## bASV_161  "g__Sphingopyxis"                                       "s__"  
## bASV_162  "g__Phenylobacterium"                                   "s__"  
## bASV_163  "g__Sphingomonas"                                       "s__"  
## bASV_164  "g__Lysobacter"                                         "s__"  
## bASV_165  "g__MSB-4B10"                                           "s__"  
## bASV_166  NA                                                      NA     
## bASV_168  NA                                                      NA     
## bASV_169  "g__Niastella"                                          "s__"  
## bASV_170  "g__Azospirillum"                                       "s__"  
## bASV_171  "g__RB41"                                               "s__"  
## bASV_173  "g__Mesorhizobium"                                      "s__"  
## bASV_174  "g__Variovorax"                                         "s__"  
## bASV_175  NA                                                      NA     
## bASV_176  "g__Rokubacteriales"                                    "s__"  
## bASV_177  "g__Fimbriimonadaceae"                                  "s__"  
## bASV_178  "g__Caulobacter"                                        "s__"  
## bASV_180  "g__Sphingomonas"                                       "s__"  
## bASV_181  "g__Kribbella"                                          "s__"  
## bASV_182  "g__Steroidobacter"                                     "s__"  
## bASV_183  NA                                                      NA     
## bASV_184  "g__Candidatus_Udaeobacter"                             "s__"  
## bASV_185  "g__uncultured"                                         "s__"  
## bASV_186  "g__Noviherbaspirillum"                                 "s__"  
## bASV_187  "g__Altererythrobacter"                                 "s__"  
## bASV_188  "g__Arenimonas"                                         "s__"  
## bASV_189  "g__Mesorhizobium"                                      "s__"  
## bASV_190  "g__Plot4-2H12"                                         "s__"  
## bASV_191  NA                                                      NA     
## bASV_192  "g__Pseudoduganella"                                    "s__"  
## bASV_193  "g__Ellin6055"                                          "s__"  
## bASV_194  "g__Ellin6055"                                          "s__"  
## bASV_195  "g__Nitrosospira"                                       "s__"  
## bASV_196  "g__Dokdonella"                                         "s__"  
## bASV_197  "g__Luteolibacter"                                      "s__"  
## bASV_198  "g__WD2101_soil_group"                                  "s__"  
## bASV_199  "g__Massilia"                                           "s__"  
## bASV_200  "g__Gemmatimonas"                                       "s__"  
## bASV_201  NA                                                      NA     
## bASV_202  "g__Lysobacter"                                         "s__"  
## bASV_203  "g__Chryseolinea"                                       "s__"  
## bASV_204  "g__TM7a"                                               "s__"  
## bASV_205  "g__Lysobacter"                                         "s__"  
## bASV_206  "g__Sphingomonas"                                       "s__"  
## bASV_207  NA                                                      NA     
## bASV_209  "g__Chthoniobacter"                                     "s__"  
## bASV_211  "g__Brevundimonas"                                      "s__"  
## bASV_212  "g__Burkholderia-Caballeronia-Paraburkholderia"         "s__"  
## bASV_213  "g__Massilia"                                           "s__"  
## bASV_214  "g__uncultured"                                         "s__"  
## bASV_215  "g__Phycicoccus"                                        "s__"  
## bASV_216  "g__Pedobacter"                                         "s__"  
## bASV_217  "g__Stenotrophomonas"                                   "s__"  
## bASV_218  "g__Ideonella"                                          "s__"  
## bASV_219  NA                                                      NA     
## bASV_220  "g__Rhizobacter"                                        "s__"  
## bASV_221  "g__Mucilaginibacter"                                   "s__"  
## bASV_222  "g__Terrabacter"                                        "s__"  
## bASV_223  NA                                                      NA     
## bASV_224  "g__Rhizobacter"                                        "s__"  
## bASV_225  "g__Dyadobacter"                                        "s__"  
## bASV_226  NA                                                      NA     
## bASV_227  "g__Subgroup_7"                                         "s__"  
## bASV_228  "g__Lysobacter"                                         "s__"  
## bASV_229  "g__uncultured"                                         "s__"  
## bASV_230  "g__Variovorax"                                         "s__"  
## bASV_231  "g__Novosphingobium"                                    "s__"  
## bASV_232  "g__Massilia"                                           "s__"  
## bASV_233  "g__Niastella"                                          "s__"  
## bASV_234  NA                                                      NA     
## bASV_235  "g__Lysobacter"                                         "s__"  
## bASV_237  "g__SC-I-84"                                            "s__"  
## bASV_238  "g__Sphingomonas"                                       "s__"  
## bASV_240  "g__WD2101_soil_group"                                  "s__"  
## bASV_241  "g__Saccharothrix"                                      "s__"  
## bASV_242  "g__Niastella"                                          "s__"  
## bASV_243  NA                                                      NA     
## bASV_244  NA                                                      NA     
## bASV_245  "g__Streptomyces"                                       "s__"  
## bASV_246  "g__Streptomyces"                                       "s__"  
## bASV_247  "g__Niastella"                                          "s__"  
## bASV_249  "g__Catellatospora"                                     "s__"  
## bASV_250  "g__Pseudoduganella"                                    "s__"  
## bASV_251  "g__Sphingomonas"                                       "s__"  
## bASV_252  "g__Sphingomonas"                                       "s__"  
## bASV_253  "g__Massilia"                                           "s__"  
## bASV_254  NA                                                      NA     
## bASV_255  NA                                                      NA     
## bASV_256  "g__Ellin6055"                                          "s__"  
## bASV_257  NA                                                      NA     
## bASV_258  "g__Massilia"                                           "s__"  
## bASV_259  "g__Herpetosiphon"                                      "s__"  
## bASV_260  "g__SC-I-84"                                            "s__"  
## bASV_261  "g__uncultured"                                         "s__"  
## bASV_262  "g__Porphyrobacter"                                     "s__"  
## bASV_263  "g__Chryseolinea"                                       "s__"  
## bASV_264  NA                                                      NA     
## bASV_265  "g__uncultured"                                         "s__"  
## bASV_266  "g__Ramlibacter"                                        "s__"  
## bASV_268  "g__Niastella"                                          "s__"  
## bASV_269  "g__Massilia"                                           "s__"  
## bASV_270  "g__Steroidobacter"                                     "s__"  
## bASV_271  "g__Reyranella"                                         "s__"  
## bASV_272  "g__Gemmatimonas"                                       "s__"  
## bASV_273  NA                                                      NA     
## bASV_274  "g__Massilia"                                           "s__"  
## bASV_275  "g__Gitt-GS-136"                                        "s__"  
## bASV_276  NA                                                      NA     
## bASV_277  NA                                                      NA     
## bASV_278  NA                                                      NA     
## bASV_279  "g__Gemmatimonas"                                       "s__"  
## bASV_280  "g__Niastella"                                          "s__"  
## bASV_281  "g__uncultured"                                         "s__"  
## bASV_282  "g__uncultured"                                         "s__"  
## bASV_283  "g__Phenylobacterium"                                   "s__"  
## bASV_285  "g__Massilia"                                           "s__"  
## bASV_286  "g__Bosea"                                              "s__"  
## bASV_288  "g__Dyella"                                             "s__"  
## bASV_289  "g__Massilia"                                           "s__"  
## bASV_290  "g__Achromobacter"                                      "s__"  
## bASV_291  "g__Lechevalieria"                                      "s__"  
## bASV_292  "g__Bacillus"                                           "s__"  
## bASV_293  "g__Massilia"                                           "s__"  
## bASV_294  NA                                                      NA     
## bASV_295  "g__Rhizobacter"                                        "s__"  
## bASV_297  "g__Asticcacaulis"                                      "s__"  
## bASV_298  NA                                                      NA     
## bASV_299  "g__JGI_0001001-H03"                                    "s__"  
## bASV_300  "g__Massilia"                                           "s__"  
## bASV_302  "g__Massilia"                                           "s__"  
## bASV_303  "g__TM7a"                                               "s__"  
## bASV_304  "g__Nitrospira"                                         "s__"  
## bASV_305  "g__Lysobacter"                                         "s__"  
## bASV_306  NA                                                      NA     
## bASV_307  "g__A21b"                                               "s__"  
## bASV_308  "g__Massilia"                                           "s__"  
## bASV_309  "g__Rhodoferax"                                         "s__"  
## bASV_310  "g__Asticcacaulis"                                      "s__"  
## bASV_312  NA                                                      NA     
## bASV_313  "g__Luteolibacter"                                      "s__"  
## bASV_314  "g__Rhizobacter"                                        "s__"  
## bASV_315  "g__Hyphomicrobium"                                     "s__"  
## bASV_316  "g__uncultured"                                         "s__"  
## bASV_317  "g__Sphingomonas"                                       "s__"  
## bASV_318  "g__Sphingomonas"                                       "s__"  
## bASV_319  "g__Acidibacter"                                        "s__"  
## bASV_321  "g__Ellin6055"                                          "s__"  
## bASV_322  "g__Lysobacter"                                         "s__"  
## bASV_323  "g__Pedobacter"                                         "s__"  
## bASV_324  "g__Dyadobacter"                                        "s__"  
## bASV_325  "g__Ralstonia"                                          "s__"  
## bASV_326  NA                                                      NA     
## bASV_327  "g__Lysobacter"                                         "s__"  
## bASV_328  "g__Massilia"                                           "s__"  
## bASV_330  "g__Niastella"                                          "s__"  
## bASV_331  "g__Rhodanobacter"                                      "s__"  
## bASV_332  "g__Gemmatimonas"                                       "s__"  
## bASV_333  "g__Emticicia"                                          "s__"  
## bASV_334  "g__Pedobacter"                                         "s__"  
## bASV_335  "g__Marmoricola"                                        "s__"  
## bASV_336  "g__Massilia"                                           "s__"  
## bASV_337  "g__Rubrivivax"                                         "s__"  
## bASV_339  "g__uncultured"                                         "s__"  
## bASV_340  NA                                                      NA     
## bASV_341  "g__Caenimonas"                                         "s__"  
## bASV_342  "g__Acidovorax"                                         "s__"  
## bASV_343  "g__Massilia"                                           "s__"  
## bASV_345  "g__Chitinophaga"                                       "s__"  
## bASV_346  "g__SC-I-84"                                            "s__"  
## bASV_347  "g__Cellvibrio"                                         "s__"  
## bASV_348  "g__Parablastomonas"                                    "s__"  
## bASV_349  NA                                                      NA     
## bASV_350  "g__Altererythrobacter"                                 "s__"  
## bASV_351  NA                                                      NA     
## bASV_352  "g__Sphingomonas"                                       "s__"  
## bASV_353  "g__Asticcacaulis"                                      "s__"  
## bASV_354  "g__Niastella"                                          "s__"  
## bASV_355  "g__Sphingomonas"                                       "s__"  
## bASV_356  "g__Variovorax"                                         "s__"  
## bASV_357  "g__Cupriavidus"                                        "s__"  
## bASV_358  "g__Arcticibacter"                                      "s__"  
## bASV_359  "g__Asticcacaulis"                                      "s__"  
## bASV_361  "g__Sphingomonas"                                       "s__"  
## bASV_362  "g__SC-I-84"                                            "s__"  
## bASV_363  "g__Chryseolinea"                                       "s__"  
## bASV_364  "g__uncultured"                                         "s__"  
## bASV_365  "g__Niastella"                                          "s__"  
## bASV_366  NA                                                      NA     
## bASV_367  "g__Gemmatimonas"                                       "s__"  
## bASV_368  "g__Rhodopseudomonas"                                   "s__"  
## bASV_369  "g__Ramlibacter"                                        "s__"  
## bASV_370  "g__Saccharimonadales"                                  "s__"  
## bASV_371  "g__Luteibacter"                                        "s__"  
## bASV_372  "g__Pelomonas"                                          "s__"  
## bASV_374  "g__Gemmatimonas"                                       "s__"  
## bASV_375  "g__Pseudoduganella"                                    "s__"  
## bASV_376  NA                                                      NA     
## bASV_377  "g__Gitt-GS-136"                                        "s__"  
## bASV_378  "g__Gemmatimonas"                                       "s__"  
## bASV_379  "g__Burkholderia-Caballeronia-Paraburkholderia"         "s__"  
## bASV_380  "g__uncultured"                                         "s__"  
## bASV_381  "g__Pseudoxanthomonas"                                  "s__"  
## bASV_382  "g__SC-I-84"                                            "s__"  
## bASV_383  NA                                                      NA     
## bASV_384  NA                                                      NA     
## bASV_385  "g__Nocardioides"                                       "s__"  
## bASV_386  "g__Novosphingobium"                                    "s__"  
## bASV_387  "g__Niastella"                                          "s__"  
## bASV_388  "g__Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium" "s__"  
## bASV_389  "g__Ellin6067"                                          "s__"  
## bASV_390  "g__Massilia"                                           "s__"  
## bASV_391  "g__Bryobacter"                                         "s__"  
## bASV_392  "g__Reyranella"                                         "s__"  
## bASV_395  "g__Gemmatimonas"                                       "s__"  
## bASV_396  "g__Massilia"                                           "s__"  
## bASV_397  "g__uncultured"                                         "s__"  
## bASV_399  "g__Caenimonas"                                         "s__"  
## bASV_400  "g__Nitrosospira"                                       "s__"  
## bASV_401  "g__Tardiphaga"                                         "s__"  
## bASV_402  NA                                                      NA     
## bASV_403  "g__Burkholderia-Caballeronia-Paraburkholderia"         "s__"  
## bASV_404  "g__Caenimonas"                                         "s__"  
## bASV_406  "g__Arenimonas"                                         "s__"  
## bASV_407  "g__Fimbriimonadaceae"                                  "s__"  
## bASV_408  "g__JGI_0001001-H03"                                    "s__"  
## bASV_409  "g__Nocardioides"                                       "s__"  
## bASV_410  "g__Burkholderia-Caballeronia-Paraburkholderia"         "s__"  
## bASV_411  "g__Rhodanobacter"                                      "s__"  
## bASV_412  "g__Ramlibacter"                                        "s__"  
## bASV_413  "g__Luteibacter"                                        "s__"  
## bASV_414  "g__Pseudomonas"                                        "s__"  
## bASV_416  "g__Ellin6067"                                          "s__"  
## bASV_417  "g__Methylobacterium-Methylorubrum"                     "s__"  
## bASV_418  "g__Methylobacterium-Methylorubrum"                     "s__"  
## bASV_419  NA                                                      NA     
## bASV_420  "g__Gaiella"                                            "s__"  
## bASV_421  "g__YC-ZSS-LKJ147"                                      "s__"  
## bASV_422  "g__Sphingomonas"                                       "s__"  
## bASV_423  "g__Duganella"                                          "s__"  
## bASV_424  "g__Lysobacter"                                         "s__"  
## bASV_425  "g__CL500-29_marine_group"                              "s__"  
## bASV_426  "g__Ellin6055"                                          "s__"  
## bASV_427  "g__uncultured"                                         "s__"  
## bASV_428  "g__Acidibacter"                                        "s__"  
## bASV_429  "g__Niastella"                                          "s__"  
## bASV_430  "g__Asticcacaulis"                                      "s__"  
## bASV_431  "g__Adhaeribacter"                                      "s__"  
## bASV_432  "g__Longimicrobiaceae"                                  "s__"  
## bASV_433  "g__Steroidobacter"                                     "s__"  
## bASV_434  "g__Marmoricola"                                        "s__"  
## bASV_435  "g__Edaphobacter"                                       "s__"  
## bASV_436  "g__Adhaeribacter"                                      "s__"  
## bASV_438  "g__Bosea"                                              "s__"  
## bASV_439  "g__uncultured"                                         "s__"  
## bASV_440  "g__SC-I-84"                                            "s__"  
## bASV_441  NA                                                      NA     
## bASV_442  "g__uncultured"                                         "s__"  
## bASV_443  "g__uncultured"                                         "s__"  
## bASV_446  "g__Altererythrobacter"                                 "s__"  
## bASV_447  NA                                                      NA     
## bASV_448  "g__Niastella"                                          "s__"  
## bASV_449  "g__Bosea"                                              "s__"  
## bASV_450  "g__Bradyrhizobium"                                     "s__"  
## bASV_451  "g__Dongia"                                             "s__"  
## bASV_453  "g__Methylotenera"                                      "s__"  
## bASV_454  "g__Blastococcus"                                       "s__"  
## bASV_455  "g__Pedobacter"                                         "s__"  
## bASV_456  "g__Nocardioides"                                       "s__"  
## bASV_457  "g__Massilia"                                           "s__"  
## bASV_458  "g__Nocardioides"                                       "s__"  
## bASV_459  "g__Mucilaginibacter"                                   "s__"  
## bASV_460  "g__Nocardioides"                                       "s__"  
## bASV_461  "g__Sphingomonas"                                       "s__"  
## bASV_462  "g__Sphingomonas"                                       "s__"  
## bASV_464  "g__Gemmatimonas"                                       "s__"  
## bASV_465  "g__WD2101_soil_group"                                  "s__"  
## bASV_466  "g__Saccharothrix"                                      "s__"  
## bASV_467  "g__uncultured"                                         "s__"  
## bASV_468  "g__Gemmatimonas"                                       "s__"  
## bASV_469  "g__MND1"                                               "s__"  
## bASV_471  NA                                                      NA     
## bASV_472  "g__Puia"                                               "s__"  
## bASV_474  NA                                                      NA     
## bASV_476  "g__Polaromonas"                                        "s__"  
## bASV_477  NA                                                      NA     
## bASV_478  "g__Duganella"                                          "s__"  
## bASV_479  "g__Lysobacter"                                         "s__"  
## bASV_480  "g__Actinocorallia"                                     "s__"  
## bASV_481  "g__uncultured"                                         "s__"  
## bASV_482  NA                                                      NA     
## bASV_483  "g__Phycicoccus"                                        "s__"  
## bASV_484  "g__Ramlibacter"                                        "s__"  
## bASV_485  "g__Massilia"                                           "s__"  
## bASV_486  "g__Niastella"                                          "s__"  
## bASV_487  "g__Massilia"                                           "s__"  
## bASV_488  "g__Frateuria"                                          "s__"  
## bASV_489  "g__Mycobacterium"                                      "s__"  
## bASV_490  "g__Lysobacter"                                         "s__"  
## bASV_491  NA                                                      NA     
## bASV_492  "g__uncultured"                                         "s__"  
## bASV_493  "g__Flavobacterium"                                     "s__"  
## bASV_494  "g__uncultured"                                         "s__"  
## bASV_495  "g__Rhizobacter"                                        "s__"  
## bASV_496  "g__Acidibacter"                                        "s__"  
## bASV_498  "g__RB41"                                               "s__"  
## bASV_499  "g__Rhizorhapis"                                        "s__"  
## bASV_500  "g__uncultured"                                         "s__"  
## bASV_501  "g__Candidatus_Solibacter"                              "s__"  
## bASV_503  "g__JGI_0001001-H03"                                    "s__"  
## bASV_504  "g__Pseudoxanthomonas"                                  "s__"  
## bASV_505  "g__Marmoricola"                                        "s__"  
## bASV_506  "g__Sphingobium"                                        "s__"  
## bASV_507  NA                                                      NA     
## bASV_508  "g__Dongia"                                             "s__"  
## bASV_509  "g__Dongia"                                             "s__"  
## bASV_510  "g__Qipengyuania"                                       "s__"  
## bASV_511  "g__Methylotenera"                                      "s__"  
## bASV_512  "g__Cellvibrio"                                         "s__"  
## bASV_513  "g__JGI_0001001-H03"                                    "s__"  
## bASV_514  NA                                                      NA     
## bASV_515  "g__Phenylobacterium"                                   "s__"  
## bASV_516  "g__Reyranella"                                         "s__"  
## bASV_517  "g__Flavobacterium"                                     "s__"  
## bASV_518  "g__Curvibacter"                                        "s__"  
## bASV_519  "g__Bryobacter"                                         "s__"  
## bASV_520  "g__Gemmatimonas"                                       "s__"  
## bASV_521  "g__Rhizobacter"                                        "s__"  
## bASV_522  "g__Niastella"                                          "s__"  
## bASV_524  "g__Novosphingobium"                                    "s__"  
## bASV_525  "g__Burkholderia-Caballeronia-Paraburkholderia"         "s__"  
## bASV_526  "g__uncultured"                                         "s__"  
## bASV_527  "g__IMCC26256"                                          "s__"  
## bASV_528  "g__Caenimonas"                                         "s__"  
## bASV_529  "g__Massilia"                                           "s__"  
## bASV_530  "g__IMCC26256"                                          "s__"  
## bASV_531  "g__uncultured"                                         "s__"  
## bASV_533  "g__Chryseobacterium"                                   "s__"  
## bASV_534  "g__uncultured"                                         "s__"  
## bASV_535  "g__Flavobacterium"                                     "s__"  
## bASV_536  "g__Nocardioides"                                       "s__"  
## bASV_537  "g__Ramlibacter"                                        "s__"  
## bASV_539  "g__Rubrivivax"                                         "s__"  
## bASV_540  NA                                                      NA     
## bASV_541  "g__Sphingomonas"                                       "s__"  
## bASV_542  "g__Glycomyces"                                         "s__"  
## bASV_546  "g__uncultured"                                         "s__"  
## bASV_547  "g__Pedobacter"                                         "s__"  
## bASV_548  "g__Lysobacter"                                         "s__"  
## bASV_549  "g__Gemmatimonas"                                       "s__"  
## bASV_550  "g__uncultured"                                         "s__"  
## bASV_551  "g__RB41"                                               "s__"  
## bASV_552  "g__Dokdonella"                                         "s__"  
## bASV_553  "g__Arcticibacter"                                      "s__"  
## bASV_554  "g__Nonomuraea"                                         "s__"  
## bASV_556  "g__Marmoricola"                                        "s__"  
## bASV_557  "g__Arenimonas"                                         "s__"  
## bASV_558  "g__Dinghuibacter"                                      "s__"  
## bASV_561  "g__Dokdonella"                                         "s__"  
## bASV_562  "g__Phenylobacterium"                                   "s__"  
## bASV_563  NA                                                      NA     
## bASV_564  "g__Chitinophaga"                                       "s__"  
## bASV_565  "g__Sphingomonas"                                       "s__"  
## bASV_567  "g__uncultured"                                         "s__"  
## bASV_569  "g__Flavobacterium"                                     "s__"  
## bASV_570  "g__uncultured"                                         "s__"  
## bASV_571  "g__IMCC26256"                                          "s__"  
## bASV_572  "g__Sphingomonas"                                       "s__"  
## bASV_573  "g__Labrys"                                             "s__"  
## bASV_574  "g__uncultured"                                         "s__"  
## bASV_575  "g__WD2101_soil_group"                                  "s__"  
## bASV_576  "g__uncultured"                                         "s__"  
## bASV_577  "g__Gemmatimonas"                                       "s__"  
## bASV_578  "g__Candidatus_Solibacter"                              "s__"  
## bASV_579  "g__KD4-96"                                             "s__"  
## bASV_580  "g__SC-I-84"                                            "s__"  
## bASV_581  "g__Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium" "s__"  
## bASV_582  "g__Rokubacteriales"                                    "s__"  
## bASV_583  "g__Candidatus_Solibacter"                              "s__"  
## bASV_584  "g__Asticcacaulis"                                      "s__"  
## bASV_585  "g__uncultured"                                         "s__"  
## bASV_586  "g__WD2101_soil_group"                                  "s__"  
## bASV_587  NA                                                      NA     
## bASV_590  "g__Massilia"                                           "s__"  
## bASV_591  "g__uncultured"                                         "s__"  
## bASV_592  "g__Cupriavidus"                                        "s__"  
## bASV_593  "g__Amycolatopsis"                                      "s__"  
## bASV_595  "g__uncultured"                                         "s__"  
## bASV_596  "g__Dyella"                                             "s__"  
## bASV_597  "g__Acidibacter"                                        "s__"  
## bASV_598  "g__Phycicoccus"                                        "s__"  
## bASV_599  NA                                                      NA     
## bASV_600  "g__Mucilaginibacter"                                   "s__"  
## bASV_601  "g__WD2101_soil_group"                                  "s__"  
## bASV_602  "g__Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium" "s__"  
## bASV_603  NA                                                      NA     
## bASV_604  "g__WD2101_soil_group"                                  "s__"  
## bASV_605  NA                                                      NA     
## bASV_606  "g__Rhizobacter"                                        "s__"  
## bASV_607  "g__Gemmatimonas"                                       "s__"  
## bASV_609  "g__Subgroup_7"                                         "s__"  
## bASV_611  "g__Sphingomonas"                                       "s__"  
## bASV_612  "g__Vicinamibacteraceae"                                "s__"  
## bASV_613  "g__Gemmatimonas"                                       "s__"  
## bASV_614  "g__Kribbella"                                          "s__"  
## bASV_615  "g__Massilia"                                           "s__"  
## bASV_616  "g__Gaiella"                                            "s__"  
## bASV_617  NA                                                      NA     
## bASV_619  "g__SC-I-84"                                            "s__"  
## bASV_621  "g__Hyphomicrobium"                                     "s__"  
## bASV_622  "g__Subgroup_7"                                         "s__"  
## bASV_623  NA                                                      NA     
## bASV_624  "g__Altererythrobacter"                                 "s__"  
## bASV_625  NA                                                      NA     
## bASV_626  NA                                                      NA     
## bASV_627  "g__Massilia"                                           "s__"  
## bASV_628  "g__Chitinophaga"                                       "s__"  
## bASV_629  "g__uncultured"                                         "s__"  
## bASV_632  "g__uncultured"                                         "s__"  
## bASV_635  NA                                                      NA     
## bASV_636  "g__Pseudoxanthomonas"                                  "s__"  
## bASV_637  "g__Gemmatimonas"                                       "s__"  
## bASV_638  "g__Haliangium"                                         "s__"  
## bASV_639  "g__Altererythrobacter"                                 "s__"  
## bASV_640  "g__Massilia"                                           "s__"  
## bASV_641  "g__Bradyrhizobium"                                     "s__"  
## bASV_642  NA                                                      NA     
## bASV_643  "g__RB41"                                               "s__"  
## bASV_645  "g__Phenylobacterium"                                   "s__"  
## bASV_646  "g__Massilia"                                           "s__"  
## bASV_647  "g__Ellin6055"                                          "s__"  
## bASV_648  "g__RB41"                                               "s__"  
## bASV_649  "g__Candidatus_Udaeobacter"                             "s__"  
## bASV_650  "g__Rhodoferax"                                         "s__"  
## bASV_651  "g__Mucilaginibacter"                                   "s__"  
## bASV_652  "g__Lysobacter"                                         "s__"  
## bASV_653  "g__Gemmatimonas"                                       "s__"  
## bASV_654  "g__Dongia"                                             "s__"  
## bASV_655  "g__Flavobacterium"                                     "s__"  
## bASV_657  "g__Pseudoduganella"                                    "s__"  
## bASV_658  "g__Paenarthrobacter"                                   "s__"  
## bASV_659  "g__67-14"                                              "s__"  
## bASV_660  "g__uncultured"                                         "s__"  
## bASV_661  "g__IMCC26256"                                          "s__"  
## bASV_662  "g__SC-I-84"                                            "s__"  
## bASV_663  "g__Pseudoduganella"                                    "s__"  
## bASV_664  "g__uncultured"                                         "s__"  
## bASV_665  NA                                                      NA     
## bASV_666  "g__Gemmatimonas"                                       "s__"  
## bASV_667  "g__MB-A2-108"                                          "s__"  
## bASV_668  "g__PLTA13"                                             "s__"  
## bASV_669  "g__Ramlibacter"                                        "s__"  
## bASV_670  "g__Subgroup_17"                                        "s__"  
## bASV_672  "g__Caenimonas"                                         "s__"  
## bASV_674  "g__WD2101_soil_group"                                  "s__"  
## bASV_675  "g__uncultured"                                         "s__"  
## bASV_676  NA                                                      NA     
## bASV_677  "g__Massilia"                                           "s__"  
## bASV_678  NA                                                      NA     
## bASV_682  "g__Brevundimonas"                                      "s__"  
## bASV_683  "g__Glycomyces"                                         "s__"  
## bASV_684  "g__Paenibacillus"                                      "s__"  
## bASV_687  "g__uncultured"                                         "s__"  
## bASV_688  "g__Acidibacter"                                        "s__"  
## bASV_691  "g__Subgroup_2"                                         "s__"  
## bASV_692  "g__AD3"                                                "s__"  
## bASV_693  "g__uncultured"                                         "s__"  
## bASV_694  "g__Rhizobacter"                                        "s__"  
## bASV_695  "g__Nocardioides"                                       "s__"  
## bASV_696  NA                                                      NA     
## bASV_698  "g__Alsobacter"                                         "s__"  
## bASV_699  "g__KD4-96"                                             "s__"  
## bASV_700  "g__Roseisolibacter"                                    "s__"  
## bASV_701  "g__Rivibacter"                                         "s__"  
## bASV_702  "g__uncultured"                                         "s__"  
## bASV_703  "g__Aquabacterium"                                      "s__"  
## bASV_704  "g__uncultured"                                         "s__"  
## bASV_705  "g__Aeromicrobium"                                      "s__"  
## bASV_706  "g__Edaphobaculum"                                      "s__"  
## bASV_707  "g__Vicinamibacteraceae"                                "s__"  
## bASV_708  "g__Pedobacter"                                         "s__"  
## bASV_709  "g__Acidibacter"                                        "s__"  
## bASV_711  "g__Nocardioides"                                       "s__"  
## bASV_712  "g__Burkholderia-Caballeronia-Paraburkholderia"         "s__"  
## bASV_713  "g__uncultured"                                         "s__"  
## bASV_714  "g__uncultured"                                         "s__"  
## bASV_715  "g__Pseudonocardia"                                     "s__"  
## bASV_716  "g__SC-I-84"                                            "s__"  
## bASV_717  "g__Niastella"                                          "s__"  
## bASV_719  "g__Cohnella"                                           "s__"  
## bASV_720  "g__uncultured"                                         "s__"  
## bASV_721  "g__uncultured"                                         "s__"  
## bASV_722  NA                                                      NA     
## bASV_724  "g__Flavobacterium"                                     "s__"  
## bASV_725  "g__Candidatus_Udaeobacter"                             "s__"  
## bASV_726  "g__IMCC26256"                                          "s__"  
## bASV_728  "g__Sphingomonas"                                       "s__"  
## bASV_729  "g__Lysobacter"                                         "s__"  
## bASV_730  "g__Massilia"                                           "s__"  
## bASV_731  "g__Duganella"                                          "s__"  
## bASV_732  "g__Candidatus_Udaeobacter"                             "s__"  
## bASV_733  "g__Candidatus_Udaeobacter"                             "s__"  
## bASV_735  "g__Fimbriimonadaceae"                                  "s__"  
## bASV_736  "g__Nitrospira"                                         "s__"  
## bASV_737  "g__uncultured"                                         "s__"  
## bASV_738  "g__Rhodoplanes"                                        "s__"  
## bASV_739  "g__Paenibacillus"                                      "s__"  
## bASV_740  "g__uncultured"                                         "s__"  
## bASV_741  "g__uncultured"                                         "s__"  
## bASV_743  "g__Niastella"                                          "s__"  
## bASV_744  "g__Pelomonas"                                          "s__"  
## bASV_745  NA                                                      NA     
## bASV_746  NA                                                      NA     
## bASV_747  "g__uncultured"                                         "s__"  
## bASV_749  "g__Hyphomicrobium"                                     "s__"  
## bASV_752  "g__Sphingomonas"                                       "s__"  
## bASV_753  "g__Vicinamibacteraceae"                                "s__"  
## bASV_754  "g__Massilia"                                           "s__"  
## bASV_755  NA                                                      NA     
## bASV_756  "g__Massilia"                                           "s__"  
## bASV_757  "g__Asticcacaulis"                                      "s__"  
## bASV_758  "g__BIrii41"                                            "s__"  
## bASV_759  "g__Pedomicrobium"                                      "s__"  
## bASV_760  "g__Sphingobium"                                        "s__"  
## bASV_761  "g__uncultured"                                         "s__"  
## bASV_762  "g__Subgroup_7"                                         "s__"  
## bASV_763  "g__WD2101_soil_group"                                  "s__"  
## bASV_764  "g__uncultured"                                         "s__"  
## bASV_766  "g__Sphingomonas"                                       "s__"  
## bASV_767  "g__Lacunisphaera"                                      "s__"  
## bASV_769  "g__Subgroup_7"                                         "s__"  
## bASV_770  "g__uncultured"                                         "s__"  
## bASV_771  NA                                                      NA     
## bASV_772  "g__Piscinibacter"                                      "s__"  
## bASV_773  NA                                                      NA     
## bASV_776  "g__mle1-27"                                            "s__"  
## bASV_777  "g__Nitrospira"                                         "s__"  
## bASV_780  "g__[Aquaspirillum]_arcticum_group"                     "s__"  
## bASV_782  "g__Nocardioides"                                       "s__"  
## bASV_784  "g__Dokdonella"                                         "s__"  
## bASV_785  "g__Massilia"                                           "s__"  
## bASV_786  "g__Caenimonas"                                         "s__"  
## bASV_787  "g__Flavisolibacter"                                    "s__"  
## bASV_788  "g__Lechevalieria"                                      "s__"  
## bASV_789  "g__Achromobacter"                                      "s__"  
## bASV_791  "g__Pedosphaeraceae"                                    "s__"  
## bASV_792  "g__Caenimonas"                                         "s__"  
## bASV_793  "g__Niastella"                                          "s__"  
## bASV_794  "g__Ellin6067"                                          "s__"  
## bASV_795  "g__uncultured"                                         "s__"  
## bASV_797  NA                                                      NA     
## bASV_798  "g__Sphingomonas"                                       "s__"  
## bASV_799  "g__C0119"                                              "s__"  
## bASV_800  "g__Roseisolibacter"                                    "s__"  
## bASV_801  NA                                                      NA     
## bASV_803  "g__Flavisolibacter"                                    "s__"  
## bASV_804  "g__Massilia"                                           "s__"  
## bASV_805  "g__Duganella"                                          "s__"  
## bASV_806  "g__Flavisolibacter"                                    "s__"  
## bASV_807  "g__RB41"                                               "s__"  
## bASV_808  "g__KD4-96"                                             "s__"  
## bASV_809  "g__Bacillus"                                           "s__"  
## bASV_810  "g__Sorangium"                                          "s__"  
## bASV_812  "g__Pseudoflavitalea"                                   "s__"  
## bASV_813  "g__Altererythrobacter"                                 "s__"  
## bASV_814  "g__Bradyrhizobium"                                     "s__"  
## bASV_816  "g__Acidibacter"                                        "s__"  
## bASV_817  "g__Burkholderia-Caballeronia-Paraburkholderia"         "s__"  
## bASV_819  "g__Kribbella"                                          "s__"  
## bASV_820  "g__Ellin6067"                                          "s__"  
## bASV_821  "g__uncultured"                                         "s__"  
## bASV_822  "g__uncultured"                                         "s__"  
## bASV_823  "g__Roseisolibacter"                                    "s__"  
## bASV_824  "g__Nocardioides"                                       "s__"  
## bASV_825  "g__Massilia"                                           "s__"  
## bASV_826  "g__A21b"                                               "s__"  
## bASV_827  "g__Arenimonas"                                         "s__"  
## bASV_828  "g__Duganella"                                          "s__"  
## bASV_829  "g__Micropepsaceae"                                     "s__"  
## bASV_830  "g__Subgroup_17"                                        "s__"  
## bASV_831  "g__Ramlibacter"                                        "s__"  
## bASV_832  "g__Arenimonas"                                         "s__"  
## bASV_833  "g__Candidatus_Udaeobacter"                             "s__"  
## bASV_835  "g__Ellin6055"                                          "s__"  
## bASV_836  "g__Massilia"                                           "s__"  
## bASV_837  "g__RB41"                                               "s__"  
## bASV_838  "g__Lysobacter"                                         "s__"  
## bASV_840  "g__uncultured"                                         "s__"  
## bASV_841  "g__Massilia"                                           "s__"  
## bASV_842  "g__Adhaeribacter"                                      "s__"  
## bASV_843  "g__Pseudoduganella"                                    "s__"  
## bASV_844  "g__MND1"                                               "s__"  
## bASV_845  NA                                                      NA     
## bASV_846  NA                                                      NA     
## bASV_847  NA                                                      NA     
## bASV_848  "g__Chryseolinea"                                       "s__"  
## bASV_849  "g__uncultured"                                         "s__"  
## bASV_851  "g__Chryseobacterium"                                   "s__"  
## bASV_854  "g__Massilia"                                           "s__"  
## bASV_855  "g__Roseiarcus"                                         "s__"  
## bASV_856  "g__uncultured"                                         "s__"  
## bASV_857  "g__Ellin6067"                                          "s__"  
## bASV_858  "g__Flavisolibacter"                                    "s__"  
## bASV_859  "g__Asticcacaulis"                                      "s__"  
## bASV_860  "g__SC-I-84"                                            "s__"  
## bASV_861  NA                                                      NA     
## bASV_865  "g__Gemmatimonas"                                       "s__"  
## bASV_866  NA                                                      NA     
## bASV_867  "g__WD2101_soil_group"                                  "s__"  
## bASV_868  "g__SC-I-84"                                            "s__"  
## bASV_869  "g__RB41"                                               "s__"  
## bASV_870  "g__Dechloromonas"                                      "s__"  
## bASV_871  "g__Paenibacillus"                                      "s__"  
## bASV_874  "g__Opitutus"                                           "s__"  
## bASV_875  "g__Lysobacter"                                         "s__"  
## bASV_876  "g__Noviherbaspirillum"                                 "s__"  
## bASV_877  "g__uncultured"                                         "s__"  
## bASV_878  "g__SC-I-84"                                            "s__"  
## bASV_879  NA                                                      NA     
## bASV_880  "g__SC-I-84"                                            "s__"  
## bASV_882  "g__Ellin6067"                                          "s__"  
## bASV_883  NA                                                      NA     
## bASV_885  "g__uncultured"                                         "s__"  
## bASV_886  "g__Deinococcus"                                        "s__"  
## bASV_887  "g__uncultured"                                         "s__"  
## bASV_888  NA                                                      NA     
## bASV_890  "g__uncultured"                                         "s__"  
## bASV_891  "g__P3OB-42"                                            "s__"  
## bASV_892  "g__Niastella"                                          "s__"  
## bASV_893  "g__A21b"                                               "s__"  
## bASV_895  "g__uncultured"                                         "s__"  
## bASV_896  "g__11-24"                                              "s__"  
## bASV_897  "g__uncultured"                                         "s__"  
## bASV_898  "g__uncultured"                                         "s__"  
## bASV_901  "g__Flavitalea"                                         "s__"  
## bASV_902  "g__uncultured"                                         "s__"  
## bASV_903  "g__Gemmata"                                            "s__"  
## bASV_904  "g__Flavobacterium"                                     "s__"  
## bASV_905  "g__Pseudonocardia"                                     "s__"  
## bASV_906  "g__uncultured"                                         "s__"  
## bASV_907  "g__Pseudomonas"                                        "s__"  
## bASV_908  "g__Phenylobacterium"                                   "s__"  
## bASV_909  "g__Massilia"                                           "s__"  
## bASV_910  "g__uncultured"                                         "s__"  
## bASV_911  "g__Ideonella"                                          "s__"  
## bASV_912  "g__Rhodoplanes"                                        "s__"  
## bASV_914  "g__Massilia"                                           "s__"  
## bASV_915  "g__Acidovorax"                                         "s__"  
## bASV_916  NA                                                      NA     
## bASV_917  "g__uncultured"                                         "s__"  
## bASV_918  "g__Flavobacterium"                                     "s__"  
## bASV_920  "g__Gemmatimonas"                                       "s__"  
## bASV_921  NA                                                      NA     
## bASV_922  NA                                                      NA     
## bASV_923  "g__Dactylosporangium"                                  "s__"  
## bASV_924  "g__WD2101_soil_group"                                  "s__"  
## bASV_925  NA                                                      NA     
## bASV_926  "g__uncultured"                                         "s__"  
## bASV_928  "g__Steroidobacter"                                     "s__"  
## bASV_930  "g__Arenimonas"                                         "s__"  
## bASV_931  "g__Candidatus_Solibacter"                              "s__"  
## bASV_933  NA                                                      NA     
## bASV_934  "g__uncultured"                                         "s__"  
## bASV_935  "g__Chthoniobacter"                                     "s__"  
## bASV_936  "g__Tepidisphaera"                                      "s__"  
## bASV_937  "g__Fluviicola"                                         "s__"  
## bASV_939  "g__Rhodoplanes"                                        "s__"  
## bASV_940  "g__Rhodoplanes"                                        "s__"  
## bASV_941  "g__SC-I-84"                                            "s__"  
## bASV_945  "g__Ellin6055"                                          "s__"  
## bASV_946  NA                                                      NA     
## bASV_947  NA                                                      NA     
## bASV_948  "g__Massilia"                                           "s__"  
## bASV_949  "g__Achromobacter"                                      "s__"  
## bASV_950  "g__Bryobacter"                                         "s__"  
## bASV_951  "g__Nordella"                                           "s__"  
## bASV_952  "g__Flectobacillus"                                     "s__"  
## bASV_953  "g__TM7a"                                               "s__"  
## bASV_954  "g__Ellin6067"                                          "s__"  
## bASV_955  "g__Bauldia"                                            "s__"  
## bASV_956  "g__uncultured"                                         "s__"  
## bASV_958  "g__uncultured"                                         "s__"  
## bASV_959  "g__IMCC26256"                                          "s__"  
## bASV_960  "g__SC-I-84"                                            "s__"  
## bASV_961  "g__Candidatus_Solibacter"                              "s__"  
## bASV_963  "g__Gemmatimonas"                                       "s__"  
## bASV_964  "g__BIrii41"                                            "s__"  
## bASV_966  "g__C0119"                                              "s__"  
## bASV_969  "g__uncultured"                                         "s__"  
## bASV_972  "g__Caulobacter"                                        "s__"  
## bASV_973  "g__WD2101_soil_group"                                  "s__"  
## bASV_974  "g__uncultured"                                         "s__"  
## bASV_975  "g__Niastella"                                          "s__"  
## bASV_976  "g__Ellin6067"                                          "s__"  
## bASV_977  "g__Subgroup_7"                                         "s__"  
## bASV_979  "g__Pedosphaeraceae"                                    "s__"  
## bASV_980  NA                                                      NA     
## bASV_981  "g__Vicinamibacteraceae"                                "s__"  
## bASV_982  "g__Arenimonas"                                         "s__"  
## bASV_983  "g__RB41"                                               "s__"  
## bASV_984  "g__Alsobacter"                                         "s__"  
## bASV_986  "g__Arenimonas"                                         "s__"  
## bASV_988  "g__Candidatus_Solibacter"                              "s__"  
## bASV_989  "g__Caenimonas"                                         "s__"  
## bASV_990  "g__Microvirga"                                         "s__"  
## bASV_991  "g__uncultured"                                         "s__"  
## bASV_992  "g__uncultured"                                         "s__"  
## bASV_994  "g__Taibaiella"                                         "s__"  
## bASV_995  "g__uncultured"                                         "s__"  
## bASV_996  "g__Arenimonas"                                         "s__"  
## bASV_998  "g__uncultured"                                         "s__"  
## bASV_999  "g__Massilia"                                           "s__"  
## bASV_1001 "g__Pseudolabrys"                                       "s__"  
## bASV_1003 "g__uncultured"                                         "s__"  
## bASV_1005 "g__Gemmatimonas"                                       "s__"  
## bASV_1006 "g__Rokubacteriales"                                    "s__"  
## bASV_1007 NA                                                      NA     
## bASV_1008 NA                                                      NA     
## bASV_1010 "g__Gemmatimonas"                                       "s__"  
## bASV_1011 "g__Saccharothrix"                                      "s__"  
## bASV_1012 "g__Iamia"                                              "s__"  
## bASV_1013 "g__uncultured"                                         "s__"  
## bASV_1014 "g__Dokdonella"                                         "s__"  
## bASV_1015 "g__Bacillus"                                           "s__"  
## bASV_1016 "g__Gemmatimonas"                                       "s__"  
## bASV_1017 "g__Ferruginibacter"                                    "s__"  
## bASV_1018 "g__uncultured"                                         "s__"  
## bASV_1019 "g__Luteolibacter"                                      "s__"  
## bASV_1020 "g__WD2101_soil_group"                                  "s__"  
## bASV_1021 "g__A21b"                                               "s__"  
## bASV_1022 "g__Vicinamibacter"                                     "s__"  
## bASV_1027 "g__Arenimonas"                                         "s__"  
## bASV_1028 "g__WD2101_soil_group"                                  "s__"  
## bASV_1029 "g__Rhizobacter"                                        "s__"  
## bASV_1030 "g__Tahibacter"                                         "s__"  
## bASV_1031 "g__Blastococcus"                                       "s__"  
## bASV_1032 NA                                                      NA     
## bASV_1033 "g__uncultured"                                         "s__"  
## bASV_1034 "g__Saccharimonadales"                                  "s__"  
## bASV_1035 "g__Pedobacter"                                         "s__"  
## bASV_1037 "g__Flavisolibacter"                                    "s__"  
## bASV_1038 "g__Cohnella"                                           "s__"  
## bASV_1039 "g__Luteolibacter"                                      "s__"  
## bASV_1041 "g__uncultured"                                         "s__"  
## bASV_1042 "g__Rhizobacter"                                        "s__"  
## bASV_1045 NA                                                      NA     
## bASV_1046 "g__Dokdonella"                                         "s__"  
## bASV_1047 "g__Ellin6067"                                          "s__"  
## bASV_1048 "g__Massilia"                                           "s__"  
## bASV_1049 "g__WD2101_soil_group"                                  "s__"  
## bASV_1052 "g__JG30-KF-AS9"                                        "s__"  
## bASV_1054 "g__Fluviicola"                                         "s__"  
## bASV_1055 "g__Blfdi19"                                            "s__"  
## bASV_1056 "g__Nocardioides"                                       "s__"  
## bASV_1057 "g__Puia"                                               "s__"  
## bASV_1058 "g__Afipia"                                             "s__"  
## bASV_1059 "g__Candidatus_Solibacter"                              "s__"  
## bASV_1060 "g__TM7a"                                               "s__"  
## bASV_1061 "g__uncultured"                                         "s__"  
## bASV_1062 NA                                                      NA     
## bASV_1064 "g__SC-I-84"                                            "s__"  
## bASV_1066 "g__uncultured"                                         "s__"  
## bASV_1067 "g__Dongia"                                             "s__"  
## bASV_1068 "g__Glycomyces"                                         "s__"  
## bASV_1070 "g__Ellin6067"                                          "s__"  
## bASV_1071 "g__SWB02"                                              "s__"  
## bASV_1072 "g__Duganella"                                          "s__"  
## bASV_1073 "g__Massilia"                                           "s__"  
## bASV_1074 "g__Nocardioides"                                       "s__"  
## bASV_1075 "g__Candidatus_Solibacter"                              "s__"  
## bASV_1077 "g__Massilia"                                           "s__"  
## bASV_1078 "g__uncultured"                                         "s__"  
## bASV_1079 "g__BIrii41"                                            "s__"  
## bASV_1080 "g__Pseudomonas"                                        "s__"  
## bASV_1081 "g__Subgroup_7"                                         "s__"  
## bASV_1082 "g__uncultured"                                         "s__"  
## bASV_1083 "g__Haliangium"                                         "s__"  
## bASV_1084 "g__Lysobacter"                                         "s__"  
## bASV_1085 "g__Vampirovibrio"                                      "s__"  
## bASV_1086 "g__uncultured"                                         "s__"  
## bASV_1087 "g__Gemmatimonas"                                       "s__"  
## bASV_1088 "g__Terrabacter"                                        "s__"  
## bASV_1089 "g__uncultured"                                         "s__"  
## bASV_1090 "g__Ramlibacter"                                        "s__"  
## bASV_1091 "g__uncultured"                                         "s__"  
## bASV_1092 "g__Nitrospira"                                         "s__"  
## bASV_1094 "g__Luteolibacter"                                      "s__"  
## bASV_1095 "g__Roseomonas"                                         "s__"  
## bASV_1096 "g__Rhodanobacter"                                      "s__"  
## bASV_1098 "g__Edaphobaculum"                                      "s__"  
## bASV_1099 NA                                                      NA     
## bASV_1102 "g__Burkholderia-Caballeronia-Paraburkholderia"         "s__"  
## bASV_1103 "g__Ramlibacter"                                        "s__"  
## bASV_1105 "g__Tepidisphaeraceae"                                  "s__"  
## bASV_1107 "g__Adhaeribacter"                                      "s__"  
## bASV_1108 "g__Opitutus"                                           "s__"  
## bASV_1109 "g__Caenimonas"                                         "s__"  
## bASV_1110 "g__Mucilaginibacter"                                   "s__"  
## bASV_1111 "g__SC-I-84"                                            "s__"  
## bASV_1112 "g__Lechevalieria"                                      "s__"  
## bASV_1114 "g__uncultured"                                         "s__"  
## bASV_1115 "g__Bryobacter"                                         "s__"  
## bASV_1116 "g__Sphingomonas"                                       "s__"  
## bASV_1117 "g__Bdellovibrio"                                       "s__"  
## bASV_1118 "g__Phenylobacterium"                                   "s__"  
## bASV_1119 "g__Sphingomonas"                                       "s__"  
## bASV_1120 NA                                                      NA     
## bASV_1121 "g__Subgroup_7"                                         "s__"  
## bASV_1122 "g__C0119"                                              "s__"  
## bASV_1123 "g__Microvirga"                                         "s__"  
## bASV_1124 NA                                                      NA     
## bASV_1125 "g__WD2101_soil_group"                                  "s__"  
## bASV_1126 "g__[Aquaspirillum]_arcticum_group"                     "s__"  
## bASV_1127 "g__Vicinamibacteraceae"                                "s__"  
## bASV_1128 "g__KF-JG30-B3"                                         "s__"  
## bASV_1129 "g__SC-I-84"                                            "s__"  
## bASV_1130 "g__uncultured"                                         "s__"  
## bASV_1131 "g__Acidibacter"                                        "s__"  
## bASV_1132 "g__uncultured"                                         "s__"  
## bASV_1133 "g__Acidovorax"                                         "s__"  
## bASV_1134 "g__Pajaroellobacter"                                   "s__"  
## bASV_1136 "g__JGI_0001001-H03"                                    "s__"  
## bASV_1137 NA                                                      NA     
##           ASV_id     
## bASV_5    "bASV_5"   
## bASV_7    "bASV_7"   
## bASV_9    "bASV_9"   
## bASV_11   "bASV_11"  
## bASV_12   "bASV_12"  
## bASV_13   "bASV_13"  
## bASV_14   "bASV_14"  
## bASV_15   "bASV_15"  
## bASV_16   "bASV_16"  
## bASV_18   "bASV_18"  
## bASV_19   "bASV_19"  
## bASV_20   "bASV_20"  
## bASV_21   "bASV_21"  
## bASV_23   "bASV_23"  
## bASV_25   "bASV_25"  
## bASV_27   "bASV_27"  
## bASV_29   "bASV_29"  
## bASV_30   "bASV_30"  
## bASV_31   "bASV_31"  
## bASV_32   "bASV_32"  
## bASV_33   "bASV_33"  
## bASV_34   "bASV_34"  
## bASV_35   "bASV_35"  
## bASV_36   "bASV_36"  
## bASV_37   "bASV_37"  
## bASV_38   "bASV_38"  
## bASV_39   "bASV_39"  
## bASV_40   "bASV_40"  
## bASV_41   "bASV_41"  
## bASV_43   "bASV_43"  
## bASV_44   "bASV_44"  
## bASV_45   "bASV_45"  
## bASV_46   "bASV_46"  
## bASV_48   "bASV_48"  
## bASV_49   "bASV_49"  
## bASV_50   "bASV_50"  
## bASV_51   "bASV_51"  
## bASV_52   "bASV_52"  
## bASV_53   "bASV_53"  
## bASV_54   "bASV_54"  
## bASV_55   "bASV_55"  
## bASV_56   "bASV_56"  
## bASV_57   "bASV_57"  
## bASV_58   "bASV_58"  
## bASV_59   "bASV_59"  
## bASV_60   "bASV_60"  
## bASV_61   "bASV_61"  
## bASV_62   "bASV_62"  
## bASV_63   "bASV_63"  
## bASV_64   "bASV_64"  
## bASV_65   "bASV_65"  
## bASV_66   "bASV_66"  
## bASV_67   "bASV_67"  
## bASV_68   "bASV_68"  
## bASV_69   "bASV_69"  
## bASV_70   "bASV_70"  
## bASV_71   "bASV_71"  
## bASV_72   "bASV_72"  
## bASV_73   "bASV_73"  
## bASV_74   "bASV_74"  
## bASV_75   "bASV_75"  
## bASV_76   "bASV_76"  
## bASV_77   "bASV_77"  
## bASV_78   "bASV_78"  
## bASV_79   "bASV_79"  
## bASV_80   "bASV_80"  
## bASV_81   "bASV_81"  
## bASV_82   "bASV_82"  
## bASV_83   "bASV_83"  
## bASV_84   "bASV_84"  
## bASV_85   "bASV_85"  
## bASV_86   "bASV_86"  
## bASV_87   "bASV_87"  
## bASV_88   "bASV_88"  
## bASV_89   "bASV_89"  
## bASV_90   "bASV_90"  
## bASV_91   "bASV_91"  
## bASV_92   "bASV_92"  
## bASV_93   "bASV_93"  
## bASV_94   "bASV_94"  
## bASV_95   "bASV_95"  
## bASV_96   "bASV_96"  
## bASV_97   "bASV_97"  
## bASV_98   "bASV_98"  
## bASV_99   "bASV_99"  
## bASV_100  "bASV_100" 
## bASV_101  "bASV_101" 
## bASV_102  "bASV_102" 
## bASV_103  "bASV_103" 
## bASV_104  "bASV_104" 
## bASV_105  "bASV_105" 
## bASV_106  "bASV_106" 
## bASV_107  "bASV_107" 
## bASV_108  "bASV_108" 
## bASV_109  "bASV_109" 
## bASV_110  "bASV_110" 
## bASV_111  "bASV_111" 
## bASV_112  "bASV_112" 
## bASV_113  "bASV_113" 
## bASV_114  "bASV_114" 
## bASV_115  "bASV_115" 
## bASV_116  "bASV_116" 
## bASV_117  "bASV_117" 
## bASV_118  "bASV_118" 
## bASV_119  "bASV_119" 
## bASV_120  "bASV_120" 
## bASV_121  "bASV_121" 
## bASV_122  "bASV_122" 
## bASV_123  "bASV_123" 
## bASV_124  "bASV_124" 
## bASV_125  "bASV_125" 
## bASV_126  "bASV_126" 
## bASV_128  "bASV_128" 
## bASV_129  "bASV_129" 
## bASV_131  "bASV_131" 
## bASV_132  "bASV_132" 
## bASV_133  "bASV_133" 
## bASV_134  "bASV_134" 
## bASV_135  "bASV_135" 
## bASV_136  "bASV_136" 
## bASV_137  "bASV_137" 
## bASV_138  "bASV_138" 
## bASV_139  "bASV_139" 
## bASV_140  "bASV_140" 
## bASV_141  "bASV_141" 
## bASV_142  "bASV_142" 
## bASV_143  "bASV_143" 
## bASV_144  "bASV_144" 
## bASV_145  "bASV_145" 
## bASV_146  "bASV_146" 
## bASV_147  "bASV_147" 
## bASV_148  "bASV_148" 
## bASV_149  "bASV_149" 
## bASV_151  "bASV_151" 
## bASV_152  "bASV_152" 
## bASV_153  "bASV_153" 
## bASV_154  "bASV_154" 
## bASV_155  "bASV_155" 
## bASV_156  "bASV_156" 
## bASV_158  "bASV_158" 
## bASV_159  "bASV_159" 
## bASV_160  "bASV_160" 
## bASV_161  "bASV_161" 
## bASV_162  "bASV_162" 
## bASV_163  "bASV_163" 
## bASV_164  "bASV_164" 
## bASV_165  "bASV_165" 
## bASV_166  "bASV_166" 
## bASV_168  "bASV_168" 
## bASV_169  "bASV_169" 
## bASV_170  "bASV_170" 
## bASV_171  "bASV_171" 
## bASV_173  "bASV_173" 
## bASV_174  "bASV_174" 
## bASV_175  "bASV_175" 
## bASV_176  "bASV_176" 
## bASV_177  "bASV_177" 
## bASV_178  "bASV_178" 
## bASV_180  "bASV_180" 
## bASV_181  "bASV_181" 
## bASV_182  "bASV_182" 
## bASV_183  "bASV_183" 
## bASV_184  "bASV_184" 
## bASV_185  "bASV_185" 
## bASV_186  "bASV_186" 
## bASV_187  "bASV_187" 
## bASV_188  "bASV_188" 
## bASV_189  "bASV_189" 
## bASV_190  "bASV_190" 
## bASV_191  "bASV_191" 
## bASV_192  "bASV_192" 
## bASV_193  "bASV_193" 
## bASV_194  "bASV_194" 
## bASV_195  "bASV_195" 
## bASV_196  "bASV_196" 
## bASV_197  "bASV_197" 
## bASV_198  "bASV_198" 
## bASV_199  "bASV_199" 
## bASV_200  "bASV_200" 
## bASV_201  "bASV_201" 
## bASV_202  "bASV_202" 
## bASV_203  "bASV_203" 
## bASV_204  "bASV_204" 
## bASV_205  "bASV_205" 
## bASV_206  "bASV_206" 
## bASV_207  "bASV_207" 
## bASV_209  "bASV_209" 
## bASV_211  "bASV_211" 
## bASV_212  "bASV_212" 
## bASV_213  "bASV_213" 
## bASV_214  "bASV_214" 
## bASV_215  "bASV_215" 
## bASV_216  "bASV_216" 
## bASV_217  "bASV_217" 
## bASV_218  "bASV_218" 
## bASV_219  "bASV_219" 
## bASV_220  "bASV_220" 
## bASV_221  "bASV_221" 
## bASV_222  "bASV_222" 
## bASV_223  "bASV_223" 
## bASV_224  "bASV_224" 
## bASV_225  "bASV_225" 
## bASV_226  "bASV_226" 
## bASV_227  "bASV_227" 
## bASV_228  "bASV_228" 
## bASV_229  "bASV_229" 
## bASV_230  "bASV_230" 
## bASV_231  "bASV_231" 
## bASV_232  "bASV_232" 
## bASV_233  "bASV_233" 
## bASV_234  "bASV_234" 
## bASV_235  "bASV_235" 
## bASV_237  "bASV_237" 
## bASV_238  "bASV_238" 
## bASV_240  "bASV_240" 
## bASV_241  "bASV_241" 
## bASV_242  "bASV_242" 
## bASV_243  "bASV_243" 
## bASV_244  "bASV_244" 
## bASV_245  "bASV_245" 
## bASV_246  "bASV_246" 
## bASV_247  "bASV_247" 
## bASV_249  "bASV_249" 
## bASV_250  "bASV_250" 
## bASV_251  "bASV_251" 
## bASV_252  "bASV_252" 
## bASV_253  "bASV_253" 
## bASV_254  "bASV_254" 
## bASV_255  "bASV_255" 
## bASV_256  "bASV_256" 
## bASV_257  "bASV_257" 
## bASV_258  "bASV_258" 
## bASV_259  "bASV_259" 
## bASV_260  "bASV_260" 
## bASV_261  "bASV_261" 
## bASV_262  "bASV_262" 
## bASV_263  "bASV_263" 
## bASV_264  "bASV_264" 
## bASV_265  "bASV_265" 
## bASV_266  "bASV_266" 
## bASV_268  "bASV_268" 
## bASV_269  "bASV_269" 
## bASV_270  "bASV_270" 
## bASV_271  "bASV_271" 
## bASV_272  "bASV_272" 
## bASV_273  "bASV_273" 
## bASV_274  "bASV_274" 
## bASV_275  "bASV_275" 
## bASV_276  "bASV_276" 
## bASV_277  "bASV_277" 
## bASV_278  "bASV_278" 
## bASV_279  "bASV_279" 
## bASV_280  "bASV_280" 
## bASV_281  "bASV_281" 
## bASV_282  "bASV_282" 
## bASV_283  "bASV_283" 
## bASV_285  "bASV_285" 
## bASV_286  "bASV_286" 
## bASV_288  "bASV_288" 
## bASV_289  "bASV_289" 
## bASV_290  "bASV_290" 
## bASV_291  "bASV_291" 
## bASV_292  "bASV_292" 
## bASV_293  "bASV_293" 
## bASV_294  "bASV_294" 
## bASV_295  "bASV_295" 
## bASV_297  "bASV_297" 
## bASV_298  "bASV_298" 
## bASV_299  "bASV_299" 
## bASV_300  "bASV_300" 
## bASV_302  "bASV_302" 
## bASV_303  "bASV_303" 
## bASV_304  "bASV_304" 
## bASV_305  "bASV_305" 
## bASV_306  "bASV_306" 
## bASV_307  "bASV_307" 
## bASV_308  "bASV_308" 
## bASV_309  "bASV_309" 
## bASV_310  "bASV_310" 
## bASV_312  "bASV_312" 
## bASV_313  "bASV_313" 
## bASV_314  "bASV_314" 
## bASV_315  "bASV_315" 
## bASV_316  "bASV_316" 
## bASV_317  "bASV_317" 
## bASV_318  "bASV_318" 
## bASV_319  "bASV_319" 
## bASV_321  "bASV_321" 
## bASV_322  "bASV_322" 
## bASV_323  "bASV_323" 
## bASV_324  "bASV_324" 
## bASV_325  "bASV_325" 
## bASV_326  "bASV_326" 
## bASV_327  "bASV_327" 
## bASV_328  "bASV_328" 
## bASV_330  "bASV_330" 
## bASV_331  "bASV_331" 
## bASV_332  "bASV_332" 
## bASV_333  "bASV_333" 
## bASV_334  "bASV_334" 
## bASV_335  "bASV_335" 
## bASV_336  "bASV_336" 
## bASV_337  "bASV_337" 
## bASV_339  "bASV_339" 
## bASV_340  "bASV_340" 
## bASV_341  "bASV_341" 
## bASV_342  "bASV_342" 
## bASV_343  "bASV_343" 
## bASV_345  "bASV_345" 
## bASV_346  "bASV_346" 
## bASV_347  "bASV_347" 
## bASV_348  "bASV_348" 
## bASV_349  "bASV_349" 
## bASV_350  "bASV_350" 
## bASV_351  "bASV_351" 
## bASV_352  "bASV_352" 
## bASV_353  "bASV_353" 
## bASV_354  "bASV_354" 
## bASV_355  "bASV_355" 
## bASV_356  "bASV_356" 
## bASV_357  "bASV_357" 
## bASV_358  "bASV_358" 
## bASV_359  "bASV_359" 
## bASV_361  "bASV_361" 
## bASV_362  "bASV_362" 
## bASV_363  "bASV_363" 
## bASV_364  "bASV_364" 
## bASV_365  "bASV_365" 
## bASV_366  "bASV_366" 
## bASV_367  "bASV_367" 
## bASV_368  "bASV_368" 
## bASV_369  "bASV_369" 
## bASV_370  "bASV_370" 
## bASV_371  "bASV_371" 
## bASV_372  "bASV_372" 
## bASV_374  "bASV_374" 
## bASV_375  "bASV_375" 
## bASV_376  "bASV_376" 
## bASV_377  "bASV_377" 
## bASV_378  "bASV_378" 
## bASV_379  "bASV_379" 
## bASV_380  "bASV_380" 
## bASV_381  "bASV_381" 
## bASV_382  "bASV_382" 
## bASV_383  "bASV_383" 
## bASV_384  "bASV_384" 
## bASV_385  "bASV_385" 
## bASV_386  "bASV_386" 
## bASV_387  "bASV_387" 
## bASV_388  "bASV_388" 
## bASV_389  "bASV_389" 
## bASV_390  "bASV_390" 
## bASV_391  "bASV_391" 
## bASV_392  "bASV_392" 
## bASV_395  "bASV_395" 
## bASV_396  "bASV_396" 
## bASV_397  "bASV_397" 
## bASV_399  "bASV_399" 
## bASV_400  "bASV_400" 
## bASV_401  "bASV_401" 
## bASV_402  "bASV_402" 
## bASV_403  "bASV_403" 
## bASV_404  "bASV_404" 
## bASV_406  "bASV_406" 
## bASV_407  "bASV_407" 
## bASV_408  "bASV_408" 
## bASV_409  "bASV_409" 
## bASV_410  "bASV_410" 
## bASV_411  "bASV_411" 
## bASV_412  "bASV_412" 
## bASV_413  "bASV_413" 
## bASV_414  "bASV_414" 
## bASV_416  "bASV_416" 
## bASV_417  "bASV_417" 
## bASV_418  "bASV_418" 
## bASV_419  "bASV_419" 
## bASV_420  "bASV_420" 
## bASV_421  "bASV_421" 
## bASV_422  "bASV_422" 
## bASV_423  "bASV_423" 
## bASV_424  "bASV_424" 
## bASV_425  "bASV_425" 
## bASV_426  "bASV_426" 
## bASV_427  "bASV_427" 
## bASV_428  "bASV_428" 
## bASV_429  "bASV_429" 
## bASV_430  "bASV_430" 
## bASV_431  "bASV_431" 
## bASV_432  "bASV_432" 
## bASV_433  "bASV_433" 
## bASV_434  "bASV_434" 
## bASV_435  "bASV_435" 
## bASV_436  "bASV_436" 
## bASV_438  "bASV_438" 
## bASV_439  "bASV_439" 
## bASV_440  "bASV_440" 
## bASV_441  "bASV_441" 
## bASV_442  "bASV_442" 
## bASV_443  "bASV_443" 
## bASV_446  "bASV_446" 
## bASV_447  "bASV_447" 
## bASV_448  "bASV_448" 
## bASV_449  "bASV_449" 
## bASV_450  "bASV_450" 
## bASV_451  "bASV_451" 
## bASV_453  "bASV_453" 
## bASV_454  "bASV_454" 
## bASV_455  "bASV_455" 
## bASV_456  "bASV_456" 
## bASV_457  "bASV_457" 
## bASV_458  "bASV_458" 
## bASV_459  "bASV_459" 
## bASV_460  "bASV_460" 
## bASV_461  "bASV_461" 
## bASV_462  "bASV_462" 
## bASV_464  "bASV_464" 
## bASV_465  "bASV_465" 
## bASV_466  "bASV_466" 
## bASV_467  "bASV_467" 
## bASV_468  "bASV_468" 
## bASV_469  "bASV_469" 
## bASV_471  "bASV_471" 
## bASV_472  "bASV_472" 
## bASV_474  "bASV_474" 
## bASV_476  "bASV_476" 
## bASV_477  "bASV_477" 
## bASV_478  "bASV_478" 
## bASV_479  "bASV_479" 
## bASV_480  "bASV_480" 
## bASV_481  "bASV_481" 
## bASV_482  "bASV_482" 
## bASV_483  "bASV_483" 
## bASV_484  "bASV_484" 
## bASV_485  "bASV_485" 
## bASV_486  "bASV_486" 
## bASV_487  "bASV_487" 
## bASV_488  "bASV_488" 
## bASV_489  "bASV_489" 
## bASV_490  "bASV_490" 
## bASV_491  "bASV_491" 
## bASV_492  "bASV_492" 
## bASV_493  "bASV_493" 
## bASV_494  "bASV_494" 
## bASV_495  "bASV_495" 
## bASV_496  "bASV_496" 
## bASV_498  "bASV_498" 
## bASV_499  "bASV_499" 
## bASV_500  "bASV_500" 
## bASV_501  "bASV_501" 
## bASV_503  "bASV_503" 
## bASV_504  "bASV_504" 
## bASV_505  "bASV_505" 
## bASV_506  "bASV_506" 
## bASV_507  "bASV_507" 
## bASV_508  "bASV_508" 
## bASV_509  "bASV_509" 
## bASV_510  "bASV_510" 
## bASV_511  "bASV_511" 
## bASV_512  "bASV_512" 
## bASV_513  "bASV_513" 
## bASV_514  "bASV_514" 
## bASV_515  "bASV_515" 
## bASV_516  "bASV_516" 
## bASV_517  "bASV_517" 
## bASV_518  "bASV_518" 
## bASV_519  "bASV_519" 
## bASV_520  "bASV_520" 
## bASV_521  "bASV_521" 
## bASV_522  "bASV_522" 
## bASV_524  "bASV_524" 
## bASV_525  "bASV_525" 
## bASV_526  "bASV_526" 
## bASV_527  "bASV_527" 
## bASV_528  "bASV_528" 
## bASV_529  "bASV_529" 
## bASV_530  "bASV_530" 
## bASV_531  "bASV_531" 
## bASV_533  "bASV_533" 
## bASV_534  "bASV_534" 
## bASV_535  "bASV_535" 
## bASV_536  "bASV_536" 
## bASV_537  "bASV_537" 
## bASV_539  "bASV_539" 
## bASV_540  "bASV_540" 
## bASV_541  "bASV_541" 
## bASV_542  "bASV_542" 
## bASV_546  "bASV_546" 
## bASV_547  "bASV_547" 
## bASV_548  "bASV_548" 
## bASV_549  "bASV_549" 
## bASV_550  "bASV_550" 
## bASV_551  "bASV_551" 
## bASV_552  "bASV_552" 
## bASV_553  "bASV_553" 
## bASV_554  "bASV_554" 
## bASV_556  "bASV_556" 
## bASV_557  "bASV_557" 
## bASV_558  "bASV_558" 
## bASV_561  "bASV_561" 
## bASV_562  "bASV_562" 
## bASV_563  "bASV_563" 
## bASV_564  "bASV_564" 
## bASV_565  "bASV_565" 
## bASV_567  "bASV_567" 
## bASV_569  "bASV_569" 
## bASV_570  "bASV_570" 
## bASV_571  "bASV_571" 
## bASV_572  "bASV_572" 
## bASV_573  "bASV_573" 
## bASV_574  "bASV_574" 
## bASV_575  "bASV_575" 
## bASV_576  "bASV_576" 
## bASV_577  "bASV_577" 
## bASV_578  "bASV_578" 
## bASV_579  "bASV_579" 
## bASV_580  "bASV_580" 
## bASV_581  "bASV_581" 
## bASV_582  "bASV_582" 
## bASV_583  "bASV_583" 
## bASV_584  "bASV_584" 
## bASV_585  "bASV_585" 
## bASV_586  "bASV_586" 
## bASV_587  "bASV_587" 
## bASV_590  "bASV_590" 
## bASV_591  "bASV_591" 
## bASV_592  "bASV_592" 
## bASV_593  "bASV_593" 
## bASV_595  "bASV_595" 
## bASV_596  "bASV_596" 
## bASV_597  "bASV_597" 
## bASV_598  "bASV_598" 
## bASV_599  "bASV_599" 
## bASV_600  "bASV_600" 
## bASV_601  "bASV_601" 
## bASV_602  "bASV_602" 
## bASV_603  "bASV_603" 
## bASV_604  "bASV_604" 
## bASV_605  "bASV_605" 
## bASV_606  "bASV_606" 
## bASV_607  "bASV_607" 
## bASV_609  "bASV_609" 
## bASV_611  "bASV_611" 
## bASV_612  "bASV_612" 
## bASV_613  "bASV_613" 
## bASV_614  "bASV_614" 
## bASV_615  "bASV_615" 
## bASV_616  "bASV_616" 
## bASV_617  "bASV_617" 
## bASV_619  "bASV_619" 
## bASV_621  "bASV_621" 
## bASV_622  "bASV_622" 
## bASV_623  "bASV_623" 
## bASV_624  "bASV_624" 
## bASV_625  "bASV_625" 
## bASV_626  "bASV_626" 
## bASV_627  "bASV_627" 
## bASV_628  "bASV_628" 
## bASV_629  "bASV_629" 
## bASV_632  "bASV_632" 
## bASV_635  "bASV_635" 
## bASV_636  "bASV_636" 
## bASV_637  "bASV_637" 
## bASV_638  "bASV_638" 
## bASV_639  "bASV_639" 
## bASV_640  "bASV_640" 
## bASV_641  "bASV_641" 
## bASV_642  "bASV_642" 
## bASV_643  "bASV_643" 
## bASV_645  "bASV_645" 
## bASV_646  "bASV_646" 
## bASV_647  "bASV_647" 
## bASV_648  "bASV_648" 
## bASV_649  "bASV_649" 
## bASV_650  "bASV_650" 
## bASV_651  "bASV_651" 
## bASV_652  "bASV_652" 
## bASV_653  "bASV_653" 
## bASV_654  "bASV_654" 
## bASV_655  "bASV_655" 
## bASV_657  "bASV_657" 
## bASV_658  "bASV_658" 
## bASV_659  "bASV_659" 
## bASV_660  "bASV_660" 
## bASV_661  "bASV_661" 
## bASV_662  "bASV_662" 
## bASV_663  "bASV_663" 
## bASV_664  "bASV_664" 
## bASV_665  "bASV_665" 
## bASV_666  "bASV_666" 
## bASV_667  "bASV_667" 
## bASV_668  "bASV_668" 
## bASV_669  "bASV_669" 
## bASV_670  "bASV_670" 
## bASV_672  "bASV_672" 
## bASV_674  "bASV_674" 
## bASV_675  "bASV_675" 
## bASV_676  "bASV_676" 
## bASV_677  "bASV_677" 
## bASV_678  "bASV_678" 
## bASV_682  "bASV_682" 
## bASV_683  "bASV_683" 
## bASV_684  "bASV_684" 
## bASV_687  "bASV_687" 
## bASV_688  "bASV_688" 
## bASV_691  "bASV_691" 
## bASV_692  "bASV_692" 
## bASV_693  "bASV_693" 
## bASV_694  "bASV_694" 
## bASV_695  "bASV_695" 
## bASV_696  "bASV_696" 
## bASV_698  "bASV_698" 
## bASV_699  "bASV_699" 
## bASV_700  "bASV_700" 
## bASV_701  "bASV_701" 
## bASV_702  "bASV_702" 
## bASV_703  "bASV_703" 
## bASV_704  "bASV_704" 
## bASV_705  "bASV_705" 
## bASV_706  "bASV_706" 
## bASV_707  "bASV_707" 
## bASV_708  "bASV_708" 
## bASV_709  "bASV_709" 
## bASV_711  "bASV_711" 
## bASV_712  "bASV_712" 
## bASV_713  "bASV_713" 
## bASV_714  "bASV_714" 
## bASV_715  "bASV_715" 
## bASV_716  "bASV_716" 
## bASV_717  "bASV_717" 
## bASV_719  "bASV_719" 
## bASV_720  "bASV_720" 
## bASV_721  "bASV_721" 
## bASV_722  "bASV_722" 
## bASV_724  "bASV_724" 
## bASV_725  "bASV_725" 
## bASV_726  "bASV_726" 
## bASV_728  "bASV_728" 
## bASV_729  "bASV_729" 
## bASV_730  "bASV_730" 
## bASV_731  "bASV_731" 
## bASV_732  "bASV_732" 
## bASV_733  "bASV_733" 
## bASV_735  "bASV_735" 
## bASV_736  "bASV_736" 
## bASV_737  "bASV_737" 
## bASV_738  "bASV_738" 
## bASV_739  "bASV_739" 
## bASV_740  "bASV_740" 
## bASV_741  "bASV_741" 
## bASV_743  "bASV_743" 
## bASV_744  "bASV_744" 
## bASV_745  "bASV_745" 
## bASV_746  "bASV_746" 
## bASV_747  "bASV_747" 
## bASV_749  "bASV_749" 
## bASV_752  "bASV_752" 
## bASV_753  "bASV_753" 
## bASV_754  "bASV_754" 
## bASV_755  "bASV_755" 
## bASV_756  "bASV_756" 
## bASV_757  "bASV_757" 
## bASV_758  "bASV_758" 
## bASV_759  "bASV_759" 
## bASV_760  "bASV_760" 
## bASV_761  "bASV_761" 
## bASV_762  "bASV_762" 
## bASV_763  "bASV_763" 
## bASV_764  "bASV_764" 
## bASV_766  "bASV_766" 
## bASV_767  "bASV_767" 
## bASV_769  "bASV_769" 
## bASV_770  "bASV_770" 
## bASV_771  "bASV_771" 
## bASV_772  "bASV_772" 
## bASV_773  "bASV_773" 
## bASV_776  "bASV_776" 
## bASV_777  "bASV_777" 
## bASV_780  "bASV_780" 
## bASV_782  "bASV_782" 
## bASV_784  "bASV_784" 
## bASV_785  "bASV_785" 
## bASV_786  "bASV_786" 
## bASV_787  "bASV_787" 
## bASV_788  "bASV_788" 
## bASV_789  "bASV_789" 
## bASV_791  "bASV_791" 
## bASV_792  "bASV_792" 
## bASV_793  "bASV_793" 
## bASV_794  "bASV_794" 
## bASV_795  "bASV_795" 
## bASV_797  "bASV_797" 
## bASV_798  "bASV_798" 
## bASV_799  "bASV_799" 
## bASV_800  "bASV_800" 
## bASV_801  "bASV_801" 
## bASV_803  "bASV_803" 
## bASV_804  "bASV_804" 
## bASV_805  "bASV_805" 
## bASV_806  "bASV_806" 
## bASV_807  "bASV_807" 
## bASV_808  "bASV_808" 
## bASV_809  "bASV_809" 
## bASV_810  "bASV_810" 
## bASV_812  "bASV_812" 
## bASV_813  "bASV_813" 
## bASV_814  "bASV_814" 
## bASV_816  "bASV_816" 
## bASV_817  "bASV_817" 
## bASV_819  "bASV_819" 
## bASV_820  "bASV_820" 
## bASV_821  "bASV_821" 
## bASV_822  "bASV_822" 
## bASV_823  "bASV_823" 
## bASV_824  "bASV_824" 
## bASV_825  "bASV_825" 
## bASV_826  "bASV_826" 
## bASV_827  "bASV_827" 
## bASV_828  "bASV_828" 
## bASV_829  "bASV_829" 
## bASV_830  "bASV_830" 
## bASV_831  "bASV_831" 
## bASV_832  "bASV_832" 
## bASV_833  "bASV_833" 
## bASV_835  "bASV_835" 
## bASV_836  "bASV_836" 
## bASV_837  "bASV_837" 
## bASV_838  "bASV_838" 
## bASV_840  "bASV_840" 
## bASV_841  "bASV_841" 
## bASV_842  "bASV_842" 
## bASV_843  "bASV_843" 
## bASV_844  "bASV_844" 
## bASV_845  "bASV_845" 
## bASV_846  "bASV_846" 
## bASV_847  "bASV_847" 
## bASV_848  "bASV_848" 
## bASV_849  "bASV_849" 
## bASV_851  "bASV_851" 
## bASV_854  "bASV_854" 
## bASV_855  "bASV_855" 
## bASV_856  "bASV_856" 
## bASV_857  "bASV_857" 
## bASV_858  "bASV_858" 
## bASV_859  "bASV_859" 
## bASV_860  "bASV_860" 
## bASV_861  "bASV_861" 
## bASV_865  "bASV_865" 
## bASV_866  "bASV_866" 
## bASV_867  "bASV_867" 
## bASV_868  "bASV_868" 
## bASV_869  "bASV_869" 
## bASV_870  "bASV_870" 
## bASV_871  "bASV_871" 
## bASV_874  "bASV_874" 
## bASV_875  "bASV_875" 
## bASV_876  "bASV_876" 
## bASV_877  "bASV_877" 
## bASV_878  "bASV_878" 
## bASV_879  "bASV_879" 
## bASV_880  "bASV_880" 
## bASV_882  "bASV_882" 
## bASV_883  "bASV_883" 
## bASV_885  "bASV_885" 
## bASV_886  "bASV_886" 
## bASV_887  "bASV_887" 
## bASV_888  "bASV_888" 
## bASV_890  "bASV_890" 
## bASV_891  "bASV_891" 
## bASV_892  "bASV_892" 
## bASV_893  "bASV_893" 
## bASV_895  "bASV_895" 
## bASV_896  "bASV_896" 
## bASV_897  "bASV_897" 
## bASV_898  "bASV_898" 
## bASV_901  "bASV_901" 
## bASV_902  "bASV_902" 
## bASV_903  "bASV_903" 
## bASV_904  "bASV_904" 
## bASV_905  "bASV_905" 
## bASV_906  "bASV_906" 
## bASV_907  "bASV_907" 
## bASV_908  "bASV_908" 
## bASV_909  "bASV_909" 
## bASV_910  "bASV_910" 
## bASV_911  "bASV_911" 
## bASV_912  "bASV_912" 
## bASV_914  "bASV_914" 
## bASV_915  "bASV_915" 
## bASV_916  "bASV_916" 
## bASV_917  "bASV_917" 
## bASV_918  "bASV_918" 
## bASV_920  "bASV_920" 
## bASV_921  "bASV_921" 
## bASV_922  "bASV_922" 
## bASV_923  "bASV_923" 
## bASV_924  "bASV_924" 
## bASV_925  "bASV_925" 
## bASV_926  "bASV_926" 
## bASV_928  "bASV_928" 
## bASV_930  "bASV_930" 
## bASV_931  "bASV_931" 
## bASV_933  "bASV_933" 
## bASV_934  "bASV_934" 
## bASV_935  "bASV_935" 
## bASV_936  "bASV_936" 
## bASV_937  "bASV_937" 
## bASV_939  "bASV_939" 
## bASV_940  "bASV_940" 
## bASV_941  "bASV_941" 
## bASV_945  "bASV_945" 
## bASV_946  "bASV_946" 
## bASV_947  "bASV_947" 
## bASV_948  "bASV_948" 
## bASV_949  "bASV_949" 
## bASV_950  "bASV_950" 
## bASV_951  "bASV_951" 
## bASV_952  "bASV_952" 
## bASV_953  "bASV_953" 
## bASV_954  "bASV_954" 
## bASV_955  "bASV_955" 
## bASV_956  "bASV_956" 
## bASV_958  "bASV_958" 
## bASV_959  "bASV_959" 
## bASV_960  "bASV_960" 
## bASV_961  "bASV_961" 
## bASV_963  "bASV_963" 
## bASV_964  "bASV_964" 
## bASV_966  "bASV_966" 
## bASV_969  "bASV_969" 
## bASV_972  "bASV_972" 
## bASV_973  "bASV_973" 
## bASV_974  "bASV_974" 
## bASV_975  "bASV_975" 
## bASV_976  "bASV_976" 
## bASV_977  "bASV_977" 
## bASV_979  "bASV_979" 
## bASV_980  "bASV_980" 
## bASV_981  "bASV_981" 
## bASV_982  "bASV_982" 
## bASV_983  "bASV_983" 
## bASV_984  "bASV_984" 
## bASV_986  "bASV_986" 
## bASV_988  "bASV_988" 
## bASV_989  "bASV_989" 
## bASV_990  "bASV_990" 
## bASV_991  "bASV_991" 
## bASV_992  "bASV_992" 
## bASV_994  "bASV_994" 
## bASV_995  "bASV_995" 
## bASV_996  "bASV_996" 
## bASV_998  "bASV_998" 
## bASV_999  "bASV_999" 
## bASV_1001 "bASV_1001"
## bASV_1003 "bASV_1003"
## bASV_1005 "bASV_1005"
## bASV_1006 "bASV_1006"
## bASV_1007 "bASV_1007"
## bASV_1008 "bASV_1008"
## bASV_1010 "bASV_1010"
## bASV_1011 "bASV_1011"
## bASV_1012 "bASV_1012"
## bASV_1013 "bASV_1013"
## bASV_1014 "bASV_1014"
## bASV_1015 "bASV_1015"
## bASV_1016 "bASV_1016"
## bASV_1017 "bASV_1017"
## bASV_1018 "bASV_1018"
## bASV_1019 "bASV_1019"
## bASV_1020 "bASV_1020"
## bASV_1021 "bASV_1021"
## bASV_1022 "bASV_1022"
## bASV_1027 "bASV_1027"
## bASV_1028 "bASV_1028"
## bASV_1029 "bASV_1029"
## bASV_1030 "bASV_1030"
## bASV_1031 "bASV_1031"
## bASV_1032 "bASV_1032"
## bASV_1033 "bASV_1033"
## bASV_1034 "bASV_1034"
## bASV_1035 "bASV_1035"
## bASV_1037 "bASV_1037"
## bASV_1038 "bASV_1038"
## bASV_1039 "bASV_1039"
## bASV_1041 "bASV_1041"
## bASV_1042 "bASV_1042"
## bASV_1045 "bASV_1045"
## bASV_1046 "bASV_1046"
## bASV_1047 "bASV_1047"
## bASV_1048 "bASV_1048"
## bASV_1049 "bASV_1049"
## bASV_1052 "bASV_1052"
## bASV_1054 "bASV_1054"
## bASV_1055 "bASV_1055"
## bASV_1056 "bASV_1056"
## bASV_1057 "bASV_1057"
## bASV_1058 "bASV_1058"
## bASV_1059 "bASV_1059"
## bASV_1060 "bASV_1060"
## bASV_1061 "bASV_1061"
## bASV_1062 "bASV_1062"
## bASV_1064 "bASV_1064"
## bASV_1066 "bASV_1066"
## bASV_1067 "bASV_1067"
## bASV_1068 "bASV_1068"
## bASV_1070 "bASV_1070"
## bASV_1071 "bASV_1071"
## bASV_1072 "bASV_1072"
## bASV_1073 "bASV_1073"
## bASV_1074 "bASV_1074"
## bASV_1075 "bASV_1075"
## bASV_1077 "bASV_1077"
## bASV_1078 "bASV_1078"
## bASV_1079 "bASV_1079"
## bASV_1080 "bASV_1080"
## bASV_1081 "bASV_1081"
## bASV_1082 "bASV_1082"
## bASV_1083 "bASV_1083"
## bASV_1084 "bASV_1084"
## bASV_1085 "bASV_1085"
## bASV_1086 "bASV_1086"
## bASV_1087 "bASV_1087"
## bASV_1088 "bASV_1088"
## bASV_1089 "bASV_1089"
## bASV_1090 "bASV_1090"
## bASV_1091 "bASV_1091"
## bASV_1092 "bASV_1092"
## bASV_1094 "bASV_1094"
## bASV_1095 "bASV_1095"
## bASV_1096 "bASV_1096"
## bASV_1098 "bASV_1098"
## bASV_1099 "bASV_1099"
## bASV_1102 "bASV_1102"
## bASV_1103 "bASV_1103"
## bASV_1105 "bASV_1105"
## bASV_1107 "bASV_1107"
## bASV_1108 "bASV_1108"
## bASV_1109 "bASV_1109"
## bASV_1110 "bASV_1110"
## bASV_1111 "bASV_1111"
## bASV_1112 "bASV_1112"
## bASV_1114 "bASV_1114"
## bASV_1115 "bASV_1115"
## bASV_1116 "bASV_1116"
## bASV_1117 "bASV_1117"
## bASV_1118 "bASV_1118"
## bASV_1119 "bASV_1119"
## bASV_1120 "bASV_1120"
## bASV_1121 "bASV_1121"
## bASV_1122 "bASV_1122"
## bASV_1123 "bASV_1123"
## bASV_1124 "bASV_1124"
## bASV_1125 "bASV_1125"
## bASV_1126 "bASV_1126"
## bASV_1127 "bASV_1127"
## bASV_1128 "bASV_1128"
## bASV_1129 "bASV_1129"
## bASV_1130 "bASV_1130"
## bASV_1131 "bASV_1131"
## bASV_1132 "bASV_1132"
## bASV_1133 "bASV_1133"
## bASV_1134 "bASV_1134"
## bASV_1136 "bASV_1136"
## bASV_1137 "bASV_1137"
# check metadata of the second list object
sample_data(object = ps_l[[2]])
##        Target Harvest_ID Block_row_column Sp_speed  Stress  Speed Block Rows
## 49_16S    16S         49            1.2.4       M1 Control Medium     1    2
## 50_16S    16S         50            2.3.1       M1 Control Medium     2    3
## 51_16S    16S         51            3.2.9       M1 Control Medium     3    2
## 52_16S    16S         52            4.1.6       M1 Control Medium     4    1
## 53_16S    16S         53           5.6.14       M1 Control Medium     5    6
## 54_16S    16S         54            6.5.3       M1 Control Medium     6    5
## 55_16S    16S         55           7.1.12       M1 Control Medium     7    1
## 56_16S    16S         56           8.2.15       M1 Control Medium     8    2
## 57_16S    16S         57            9.2.7       M1 Control Medium     9    2
## 58_16S    16S         58          10.2.11       M1 Control Medium    10    2
## 59_16S    16S         59           11.1.2       M1 Control Medium    11    1
## 60_16S    16S         60          12.5.13       M1 Control Medium    12    5
## 61_16S    16S         61          13.4.10       M1 Control Medium    13    4
## 62_16S    16S         62           14.4.5       M1 Control Medium    14    4
## 63_16S    16S         63           15.6.8       M1 Control Medium    15    6
## 64_16S    16S         64          16.1.16       M1 Control Medium    16    1
## 65_16S    16S         65           1.6.10       M1    MeJA Medium     1    6
## 66_16S    16S         66            2.6.2       M1    MeJA Medium     2    6
## 67_16S    16S         67           3.2.13       M1    MeJA Medium     3    2
## 68_16S    16S         68            4.3.8       M1    MeJA Medium     4    3
## 69_16S    16S         69           5.5.14       M1    MeJA Medium     5    5
## 70_16S    16S         70            6.5.4       M1    MeJA Medium     6    5
## 71_16S    16S         71            7.2.3       M1    MeJA Medium     7    2
## 72_16S    16S         72            8.1.1       M1    MeJA Medium     8    1
## 73_16S    16S         73           9.6.16       M1    MeJA Medium     9    6
## 74_16S    16S         74          10.3.11       M1    MeJA Medium    10    3
## 75_16S    16S         75           11.5.9       M1    MeJA Medium    11    5
## 76_16S    16S         76          12.5.17       M1    MeJA Medium    12    5
## 77_16S    16S         77          13.2.12       M1    MeJA Medium    13    2
## 78_16S    16S         78           14.6.6       M1    MeJA Medium    14    6
## 79_16S    16S         79           15.6.7       M1    MeJA Medium    15    6
## 80_16S    16S         80          16.3.15       M1    MeJA Medium    16    3
## 81_16S    16S         81            1.4.6       M1      SA Medium     1    4
## 82_16S    16S         82            2.2.9       M1      SA Medium     2    2
## 83_16S    16S         83           3.4.10       M1      SA Medium     3    4
## 84_16S    16S         84            4.2.2       M1      SA Medium     4    2
## 85_16S    16S         85            5.4.1       M1      SA Medium     5    4
## 86_16S    16S         86           6.2.17       M1      SA Medium     6    2
## 87_16S    16S         87           7.2.13       M1      SA Medium     7    2
## 88_16S    16S         88           8.5.15       M1      SA Medium     8    5
## 89_16S    16S         89            9.6.7       M1      SA Medium     9    6
## 90_16S    16S         90          10.5.11       M1      SA Medium    10    5
## 91_16S    16S         91          11.1.12       M1      SA Medium    11    1
## 92_16S    16S         92           12.6.5       M1      SA Medium    12    6
## 93_16S    16S         93          13.6.14       M1      SA Medium    13    6
## 94_16S    16S         94           14.2.3       M1      SA Medium    14    2
## 95_16S    16S         95          15.2.16       M1      SA Medium    15    2
## 96_16S    16S         96           16.3.8       M1      SA Medium    16    3
##        Columns root_weight Nucleic_Acid_ngul Plate_name Plate_postion Plate_row
## 49_16S       4   0.4940625              73.3         M1            A1         A
## 50_16S       1   0.3940625              71.5         M1            B1         B
## 51_16S       9   0.4840625             124.3         M1            C1         C
## 52_16S       6   0.7640625              86.4         M1            D1         D
## 53_16S      14   0.4940625             103.9         M1            E1         E
## 54_16S       3   0.5140625              43.1         M1            F1         F
## 55_16S      12   0.4440625              66.0         M1            G1         G
## 56_16S      15   0.4740625             101.7         M1            H1         H
## 57_16S       7   0.3040625             112.6         M1            A2         A
## 58_16S      11   0.3240625              98.6         M1            B2         B
## 59_16S       2   0.3340625              59.8         M1            C2         C
## 60_16S      13   0.3040625             103.7         M1            D2         D
## 61_16S      10   0.6840625              55.4         M1            E2         E
## 62_16S       5   0.4040625              77.1         M1            F2         F
## 63_16S       8   0.3840625              55.2         M1            G2         G
## 64_16S      16   0.5840625              97.4         M1            H2         H
## 65_16S      10   0.6340625              67.8         M1            A3         A
## 66_16S       2   0.2340625              37.2         M1            B3         B
## 67_16S      13   0.3940625              69.4         M1            C3         C
## 68_16S       8   0.2540625              59.3         M1            D3         D
## 69_16S      14   0.3540625              48.0         M1            E3         E
## 70_16S       4   0.4740625              58.7         M1            F3         F
## 71_16S       3   0.4340625              50.9         M1            G3         G
## 72_16S       1   0.3140625              61.6         M1            H3         H
## 73_16S      16   0.3640625              72.3         M1            A4         A
## 74_16S      11   0.2940625              72.9         M1            B4         B
## 75_16S       9   0.4340625              85.8         M1            C4         C
## 76_16S      17   0.3640625              14.6         M1            D4         D
## 77_16S      12   0.2640625              71.2         M1            E4         E
## 78_16S       6   0.2440625              50.3         M1            F4         F
## 79_16S       7   0.3240625              52.1         M1            G4         G
## 80_16S      15   0.6440625              64.3         M1            H4         H
## 81_16S       6   0.4840625              88.7         M1            A5         A
## 82_16S       9   0.7240625             108.8         M1            B5         B
## 83_16S      10   0.7440625              77.6         M1            C5         C
## 84_16S       2   0.4440625              92.3         M1            D5         D
## 85_16S       1   0.3440625              79.1         M1            E5         E
## 86_16S      17   0.3040625              63.3         M1            F5         F
## 87_16S      13   0.2940625              46.3         M1            G5         G
## 88_16S      15   0.4740625              93.1         M1            H5         H
## 89_16S       7   0.2640625               9.9         M1            A6         A
## 90_16S      11   0.5340625              87.1         M1            B6         B
## 91_16S      12   0.3840625              86.4         M1            C6         C
## 92_16S       5   0.4840625              93.5         M1            D6         D
## 93_16S      14   0.7540625              97.4         M1            E6         E
## 94_16S       3   0.6340625              77.4         M1            F6         F
## 95_16S      16   0.6440625              88.1         M1            G6         G
## 96_16S       8   0.4040625              91.1         M1            H6         H
##        Plate_column GQ_submission_order sample_type greenhouse_compartment
## 49_16S            1                1346  rhizoplane                   2.12
## 50_16S            1                1347  rhizoplane                   2.12
## 51_16S            1                1348  rhizoplane                   2.12
## 52_16S            1                1349  rhizoplane                   2.12
## 53_16S            1                1350  rhizoplane                   2.12
## 54_16S            1                1351  rhizoplane                   2.12
## 55_16S            1                1352  rhizoplane                   2.12
## 56_16S            1                1353  rhizoplane                   2.12
## 57_16S            2                1354  rhizoplane                   2.10
## 58_16S            2                1355  rhizoplane                   2.10
## 59_16S            2                1356  rhizoplane                   2.10
## 60_16S            2                1357  rhizoplane                   2.10
## 61_16S            2                1358  rhizoplane                   2.10
## 62_16S            2                1359  rhizoplane                   2.10
## 63_16S            2                1360  rhizoplane                   2.10
## 64_16S            2                1361  rhizoplane                   2.10
## 65_16S            3                1362  rhizoplane                   2.12
## 66_16S            3                1363  rhizoplane                   2.12
## 67_16S            3                1364  rhizoplane                   2.12
## 68_16S            3                1365  rhizoplane                   2.12
## 69_16S            3                1366  rhizoplane                   2.12
## 70_16S            3                1367  rhizoplane                   2.12
## 71_16S            3                1368  rhizoplane                   2.12
## 72_16S            3                1369  rhizoplane                   2.12
## 73_16S            4                1370  rhizoplane                   2.10
## 74_16S            4                1371  rhizoplane                   2.10
## 75_16S            4                1372  rhizoplane                   2.10
## 76_16S            4                1373  rhizoplane                   2.10
## 77_16S            4                1374  rhizoplane                   2.10
## 78_16S            4                1375  rhizoplane                   2.10
## 79_16S            4                1376  rhizoplane                   2.10
## 80_16S            4                1377  rhizoplane                   2.10
## 81_16S            5                1378  rhizoplane                   2.12
## 82_16S            5                1379  rhizoplane                   2.12
## 83_16S            5                1380  rhizoplane                   2.12
## 84_16S            5                1381  rhizoplane                   2.12
## 85_16S            5                1382  rhizoplane                   2.12
## 86_16S            5                1383  rhizoplane                   2.12
## 87_16S            5                1384  rhizoplane                   2.12
## 88_16S            5                1385  rhizoplane                   2.12
## 89_16S            6                1386  rhizoplane                   2.10
## 90_16S            6                1387  rhizoplane                   2.10
## 91_16S            6                1388  rhizoplane                   2.10
## 92_16S            6                1389  rhizoplane                   2.10
## 93_16S            6                1390  rhizoplane                   2.10
## 94_16S            6                1391  rhizoplane                   2.10
## 95_16S            6                1392  rhizoplane                   2.10
## 96_16S            6                1393  rhizoplane                   2.10
##        greenhouse_table                     Sp_full_name Sp_abb_name   Sp_Tribe
## 49_16S              1_2 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 50_16S              1_2 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 51_16S              3_4 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 52_16S              3_4 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 53_16S              5_6 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 54_16S              5_6 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 55_16S              7_8 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 56_16S              7_8 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 57_16S             9_10 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 58_16S             9_10 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 59_16S            11_12 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 60_16S            11_12 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 61_16S            13_14 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 62_16S            13_14 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 63_16S            15_16 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 64_16S            15_16 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 65_16S              1_2 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 66_16S              1_2 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 67_16S              3_4 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 68_16S              3_4 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 69_16S              5_6 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 70_16S              5_6 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 71_16S              7_8 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 72_16S              7_8 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 73_16S             9_10 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 74_16S             9_10 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 75_16S            11_12 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 76_16S            11_12 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 77_16S            13_14 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 78_16S            13_14 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 79_16S            15_16 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 80_16S            15_16 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 81_16S              1_2 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 82_16S              1_2 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 83_16S              3_4 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 84_16S              3_4 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 85_16S              5_6 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 86_16S              5_6 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 87_16S              7_8 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 88_16S              7_8 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 89_16S             9_10 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 90_16S             9_10 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 91_16S            11_12 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 92_16S            11_12 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 93_16S            13_14 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 94_16S            13_14 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 95_16S            15_16 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
## 96_16S            15_16 Brassica_oleraceae_Rivera_Medium        Bo_M Brassiceae
##        Sp_Lineage_Walden    Sp_Family Sp_Guest Roots Shoot_.DryWeight Rainbow
## 49_16S        lineage_II Brassicaceae       no     1            1.016       0
## 50_16S        lineage_II Brassicaceae       no     1            1.179       0
## 51_16S        lineage_II Brassicaceae       no     1            1.391       0
## 52_16S        lineage_II Brassicaceae       no     1            1.436       0
## 53_16S        lineage_II Brassicaceae       no     1            1.048       0
## 54_16S        lineage_II Brassicaceae       no     1            1.227       0
## 55_16S        lineage_II Brassicaceae       no     1               NA       1
## 56_16S        lineage_II Brassicaceae       no     1               NA       1
## 57_16S        lineage_II Brassicaceae       no     1            1.728       0
## 58_16S        lineage_II Brassicaceae       no     1            1.498       0
## 59_16S        lineage_II Brassicaceae       no     1               NA       1
## 60_16S        lineage_II Brassicaceae       no     1            1.582       0
## 61_16S        lineage_II Brassicaceae       no     1            1.514       0
## 62_16S        lineage_II Brassicaceae       no     1               NA       1
## 63_16S        lineage_II Brassicaceae       no     1            1.313       0
## 64_16S        lineage_II Brassicaceae       no     1            1.632       0
## 65_16S        lineage_II Brassicaceae       no     1            0.955       0
## 66_16S        lineage_II Brassicaceae       no     1            1.065       0
## 67_16S        lineage_II Brassicaceae       no     1            0.897       0
## 68_16S        lineage_II Brassicaceae       no     1            0.999       0
## 69_16S        lineage_II Brassicaceae       no     1            0.749       0
## 70_16S        lineage_II Brassicaceae       no     1            1.030       0
## 71_16S        lineage_II Brassicaceae       no     1               NA       1
## 72_16S        lineage_II Brassicaceae       no     1               NA       1
## 73_16S        lineage_II Brassicaceae       no     1            1.265       0
## 74_16S        lineage_II Brassicaceae       no     1            1.197       0
## 75_16S        lineage_II Brassicaceae       no     1               NA       1
## 76_16S        lineage_II Brassicaceae       no     1            1.275       0
## 77_16S        lineage_II Brassicaceae       no     1            1.175       0
## 78_16S        lineage_II Brassicaceae       no     1               NA       1
## 79_16S        lineage_II Brassicaceae       no     1            1.161       0
## 80_16S        lineage_II Brassicaceae       no     1            1.115       0
## 81_16S        lineage_II Brassicaceae       no     1            1.156       0
## 82_16S        lineage_II Brassicaceae       no     1            1.617       0
## 83_16S        lineage_II Brassicaceae       no     1            1.352       0
## 84_16S        lineage_II Brassicaceae       no     1            1.102       0
## 85_16S        lineage_II Brassicaceae       no     1            1.240       0
## 86_16S        lineage_II Brassicaceae       no     1            1.307       0
## 87_16S        lineage_II Brassicaceae       no     1               NA       1
## 88_16S        lineage_II Brassicaceae       no     1               NA       1
## 89_16S        lineage_II Brassicaceae       no     1            1.417       0
## 90_16S        lineage_II Brassicaceae       no     1            1.099       0
## 91_16S        lineage_II Brassicaceae       no     1               NA       1
## 92_16S        lineage_II Brassicaceae       no     1            1.474       0
## 93_16S        lineage_II Brassicaceae       no     1            1.579       0
## 94_16S        lineage_II Brassicaceae       no     1               NA       1
## 95_16S        lineage_II Brassicaceae       no     1            1.521       0
## 96_16S        lineage_II Brassicaceae       no     1            1.268       0
##        Broken Flowering soil_moisture Missing                   Notes_1 Notes_2
## 49_16S      0         0        normal       0                                  
## 50_16S      0         0        normal       0                                  
## 51_16S      0         0        normal       0                                  
## 52_16S      1         0        normal       0                                  
## 53_16S      0         0        normal       0                                  
## 54_16S      0         0        normal       0                                  
## 55_16S      0         0        normal       0                                  
## 56_16S      1         0        normal       0                                  
## 57_16S      0         0        normal       0                                  
## 58_16S      0         0        normal       0                                  
## 59_16S      1         0        normal       0                                  
## 60_16S      0         0        normal       0                                  
## 61_16S      0         0        normal       0                                  
## 62_16S      0         0        normal       0                                  
## 63_16S      0         0        normal       0                                  
## 64_16S      0         0        normal       0                                  
## 65_16S      0         0        normal       0                                  
## 66_16S      0         0        normal       0                                  
## 67_16S      0         0        normal       0                                  
## 68_16S      0         0        normal       0                                  
## 69_16S      1         0        normal       0                                  
## 70_16S      0         0        normal       0                                  
## 71_16S      0         0        normal       0                                  
## 72_16S      0         0        normal       0                                  
## 73_16S      1         0        normal       0                                  
## 74_16S      0         0        normal       0                                  
## 75_16S      0         0        normal       0                                  
## 76_16S      0         0        normal       0                                  
## 77_16S      0         0        normal       0                                  
## 78_16S      1         0        normal       0                                  
## 79_16S      0         0        normal       0                                  
## 80_16S      0         0        normal       0                                  
## 81_16S      1         0        normal       0 SA on soil on 1st dipping        
## 82_16S      0         0        normal       0                                  
## 83_16S      0         0        normal       0                                  
## 84_16S      0         0        normal       0                                  
## 85_16S      0         0        normal       0                                  
## 86_16S      0         0        normal       0                                  
## 87_16S      0         0        normal       0                                  
## 88_16S      1         0        normal       0                                  
## 89_16S      0         0        normal       0                                  
## 90_16S      0         0        normal       0                                  
## 91_16S      0         0        normal       0                                  
## 92_16S      0         0        normal       0                                  
## 93_16S      0         0        normal       0                                  
## 94_16S      0         0        normal       0                                  
## 95_16S      0         0        normal       0                                  
## 96_16S      0         0        normal       0                                  
##          Rainbow.batch         barcode Amplicon_concentation_ngul
## 49_16S                 VAL12505782-A01                       29.1
## 50_16S                 VAL12505782-B01                       31.8
## 51_16S                 VAL12505782-C01                       32.2
## 52_16S                 VAL12505782-D01                       32.2
## 53_16S                 VAL12505782-E01                       33.9
## 54_16S                 VAL12505782-F01                       30.7
## 55_16S  PPH 17-21_June VAL12505782-G01                       28.9
## 56_16S  PPH 17-21_June VAL12505782-H01                       30.1
## 57_16S                 VAL12505782-A02                       30.0
## 58_16S                 VAL12505782-B02                       31.1
## 59_16S  PPH 17-21_June VAL12505782-C02                       31.7
## 60_16S                 VAL12505782-D02                       33.8
## 61_16S                 VAL12505782-E02                       32.0
## 62_16S  PPH 17-21_June VAL12505782-F02                       32.1
## 63_16S                 VAL12505782-G02                       33.5
## 64_16S                 VAL12505782-H02                       35.9
## 65_16S                 VAL12505782-A03                       30.7
## 66_16S                 VAL12505782-B03                       30.9
## 67_16S                 VAL12505782-C03                       32.3
## 68_16S                 VAL12505782-D03                       33.4
## 69_16S                 VAL12505782-E03                       31.7
## 70_16S                 VAL12505782-F03                       29.1
## 71_16S  PPH 17-21_June VAL12505782-G03                       29.4
## 72_16S Agros 1st batch VAL12505782-H03                       28.9
## 73_16S                 VAL12505782-A04                       29.7
## 74_16S                 VAL12505782-B04                       31.9
## 75_16S  PPH 17-21_June VAL12505782-C04                       31.8
## 76_16S                 VAL12505782-D04                       28.8
## 77_16S                 VAL12505782-E04                       33.6
## 78_16S  PPH 17-21_June VAL12505782-F04                       31.0
## 79_16S                 VAL12505782-G04                       28.1
## 80_16S                 VAL12505782-H04                       28.3
## 81_16S                 VAL12505782-A05                       32.5
## 82_16S                 VAL12505782-B05                       30.1
## 83_16S                 VAL12505782-C05                       30.8
## 84_16S                 VAL12505782-D05                       31.1
## 85_16S                 VAL12505782-E05                       33.2
## 86_16S                 VAL12505782-F05                       30.8
## 87_16S  PPH 17-21_June VAL12505782-G05                       29.8
## 88_16S  PPH 17-21_June VAL12505782-H05                       30.1
## 89_16S                 VAL12505782-A06                       29.5
## 90_16S                 VAL12505782-B06                       30.8
## 91_16S Agros 1st batch VAL12505782-C06                       32.5
## 92_16S                 VAL12505782-D06                       33.0
## 93_16S                 VAL12505782-E06                       34.1
## 94_16S  PPH 17-21_June VAL12505782-F06                       33.2
## 95_16S                 VAL12505782-G06                       32.3
## 96_16S                 VAL12505782-H06                       32.0
##        library_sizes_prefiltering Mitochondria_reads Plastid_reads
## 49_16S                     116195               6573         27990
## 50_16S                     123590               7282         36998
## 51_16S                     113057               7938         35448
## 52_16S                     142608              14650         64867
## 53_16S                     152248              14715         77408
## 54_16S                     181974              18272         73214
## 55_16S                     127041               3317         13972
## 56_16S                      85303               3994         17840
## 57_16S                     128903               8443         42877
## 58_16S                      94056               1622          7398
## 59_16S                     124510               5566         32097
## 60_16S                     120863              15075         57324
## 61_16S                     139780               8013         46233
## 62_16S                      95978               3109         13858
## 63_16S                     121338               6860         28353
## 64_16S                      81246               3107         14097
## 65_16S                     159878              10964         52030
## 66_16S                     135297               3842         23341
## 67_16S                     112635               6132         33454
## 68_16S                     134955               9353         47001
## 69_16S                     154678              10334         53780
## 70_16S                     134113               4307         20843
## 71_16S                     113923               4235         17826
## 72_16S                     115794               4086         18045
## 73_16S                     134089               2611         10743
## 74_16S                     137066               6384         39736
## 75_16S                     118530               6177         29354
## 76_16S                     185816              15673         77683
## 77_16S                     134286              13365         50772
## 78_16S                     114245               1990         10657
## 79_16S                     117978               6277         28828
## 80_16S                     111556               2097          8014
## 81_16S                     107011               2407         12219
## 82_16S                      95294               2415          9354
## 83_16S                     115787               3798         15422
## 84_16S                      90829               2414         15303
## 85_16S                      93732               3805         18119
## 86_16S                     126500               7946         35750
## 87_16S                     111569               3965         16313
## 88_16S                     101406               2269          9562
## 89_16S                     163241              11136         45104
## 90_16S                     108694               1933          8411
## 91_16S                     103902               3489         19724
## 92_16S                     115149               2759         10647
## 93_16S                      91036               3239         15840
## 94_16S                      82835                880          2817
## 95_16S                      86713               3390         13844
## 96_16S                     122287               5320         21564
##        Host_DNA_n_reads Host_DNA_contamination_pct library_size is.neg
## 49_16S            34563                  30.078322        80347  FALSE
## 50_16S            44280                  36.016105        78665  FALSE
## 51_16S            43386                  38.762765        68541  FALSE
## 52_16S            79517                  56.177894        62028  FALSE
## 53_16S            92123                  60.978322        58952  FALSE
## 54_16S            91486                  50.877564        88323  FALSE
## 55_16S            17289                  13.719142       108727  FALSE
## 56_16S            21834                  25.781084        62856  FALSE
## 57_16S            51320                  40.085608        76706  FALSE
## 58_16S             9020                   9.659559        84359  FALSE
## 59_16S            37663                  30.450249        86024  FALSE
## 60_16S            72399                  60.303856        47658  FALSE
## 61_16S            54246                  39.189140        84175  FALSE
## 62_16S            16967                  17.899567        77805  FALSE
## 63_16S            35213                  29.209317        85341  FALSE
## 64_16S            17204                  21.337765        63419  FALSE
## 65_16S            62994                  39.681260        95756  FALSE
## 66_16S            27183                  20.314473       106628  FALSE
## 67_16S            39586                  35.396790        72245  FALSE
## 68_16S            56354                  42.031699        77721  FALSE
## 69_16S            64114                  41.882129        88960  FALSE
## 70_16S            25150                  19.080784       106658  FALSE
## 71_16S            22061                  19.527675        90911  FALSE
## 72_16S            22131                  19.259253        92780  FALSE
## 73_16S            13354                  10.110845       118722  FALSE
## 74_16S            46120                  33.913760        89865  FALSE
## 75_16S            35531                  30.293807        81741  FALSE
## 76_16S            93356                  50.726480        90573  FALSE
## 77_16S            64137                  48.089165        69225  FALSE
## 78_16S            12647                  11.149313       100770  FALSE
## 79_16S            35105                  30.369223        80452  FALSE
## 80_16S            10111                   9.167483       100181  FALSE
## 81_16S            14626                  13.838192        91067  FALSE
## 82_16S            11769                  12.457923        82699  FALSE
## 83_16S            19220                  16.797616        95199  FALSE
## 84_16S            17717                  19.733794        72063  FALSE
## 85_16S            21924                  23.586875        71023  FALSE
## 86_16S            43696                  34.880342        81567  FALSE
## 87_16S            20278                  18.316653        90422  FALSE
## 88_16S            11831                  11.849604        88011  FALSE
## 89_16S            56240                  34.948609       104479  FALSE
## 90_16S            10344                   9.644755        96906  FALSE
## 91_16S            23213                  22.540395        79771  FALSE
## 92_16S            13406                  11.808541       100120  FALSE
## 93_16S            19079                  21.151650        71122  FALSE
## 94_16S             3697                   4.506833        78334  FALSE
## 95_16S            17234                  20.068237        68638  FALSE
## 96_16S            26884                  22.212124        94149  FALSE

4.4 - run custom function on list of phyloseq objects

lapply (list apply) will apply a function over a list. as we have a list of phyloseq objects now, it will be easy to produce several matrixes of heat trees. when working on lists of 33 species we learned that 33 plots are hardly helpful. still, the code and the concept (make a custom function that uses a phyloseq object as input, then run over a list with lapply) can be very useful on your own analysis

# are you new to lapply? see here how it works. you firt provide a list, and then a function you will use independently on each element x of your list. here we siply count the number of taxa of each of the 3 phyloseq objects in the list
lapply(ps_l, function(x)  
  ntaxa(x))
## $At
## [1] 963
## 
## $Bo_M
## [1] 994
## 
## $It
## [1] 988
# run the custom function over a list of phyloseq objects... it can take a moment
heat_tree_stress_l<-lapply(ps_l, function(x)  
  phyloseq_to_heat_tree_matrix (ps_object = x, sample_group = "Stress"))

QUESTION BREAK

5.0 - adding external data to the heat trees

What if we want to visualize other types of taxa-associated data, like p values from deseq2, importance in random forest predictions, distances in an ordination, or degree in a network? You can do that, as long as the vector of values you use as node_size or node_colour have the same lenght as the number of taxon names you are plotting. You can for example add another data layer to the taxmap object, or provide data from an independent df as the argument of node_size or node_colour.

5.1 - check some input arguments of a heat tree

TO explore this, lets first check some of the taxmap object structure, then create some random data and add it to the heat tree

# let's review one of the more basic trees.
taxmap_obj %>%
  heat_tree(node_label = taxon_names,
            node_size = n_obs,
            node_color = n_obs,
            layout = "davidson-harel",
            initial_layout = "reingold-tilford")

#let's review what is actually used as input for node size and color
n_obs(taxmap_obj)
##   ab   ac   ad   ae   af   ag   ah   ai   aj   ak   al   am   an   ap   aq   ar 
## 1000  574  102   84   79   59   15   11    3    3   18   21    8   14    4    1 
##   as   at   au   av   aw   ax   ay   az   ba   bb   bc   bd   be   bf   bg   bh 
##    1    1  359  102  215   64   23   57   13   11   24    3    3   18   18    8 
##   bj   bk   bl   bm   bn   bo   bp   bq   br   bs   bt   bu   bv   bw   bx   by 
##    8    4    2    4    2    5    2   14   24    3    1    1    3    4    1    1 
##   bz   ca   cb   cc   cd   ce   cf   cg   ch   ci   cj   ck   cl   cm   cn   co 
##    1  272    8   53   77    9   82   32   10   62   15    8   57   11   21    1 
##   cp   cq   cr   cs   ct   cu   cv   cw   cx   cy   cz   da   db   dc   de   df 
##    5   17   17    1    6    1    9    3    3    6    8    5   18    8    8   14 
##   dg   dh   di   dj   dk   dl   dm   dn   do   dp   dq   dr   ds   dt   du   dv 
##    2    4    3    2    4    8    2    2    4    2    5   22    8    5    5    2 
##   dw   dx   dy   dz   ea   eb   ec   ed   ee   ef   eg   eh   ei   ej   ek   el 
##    7    1    3    9    6    3    3    2    1    1    1    2    5    1    1    2 
##   em   en   eo   ep   eq   er   es   et   eu   ew   ex   ey   ez   fa   fc   fd 
##    3    1    2    3    1    2    1    1    1    1    1    1    1    1  100    8 
##   fe   ff   fg   fh   fi   fj   fk   fl   fm   fn   fo   fp   fq   fr   fs   ft 
##   52   77    9   25  111   29   31    4    3   44    7    9   18    4    6   57 
##   fu   fv   fw   fx   fy   fz   ga   gb   gc   gd   ge   gf   gg   gh   gi   gj 
##   11   21    1    5   15    4   17    1    6    1    9    3    3    6    8   14 
##   gk   gl   gm   gn   gp   gq   gr   gs   gt   gu   gv   gw   gx   gy   gz   ha 
##    5   16    4    7    4    8   14   19    2    1    3    2    4    4    4    5 
##   hb   hc   hd   he   hf   hg   hh   hi   hj   hk   hl   hm   hn   ho   hp   hq 
##    8    2   10    3    2    2    2    2    2    8    4    2    8    3    5    2 
##   hr   hs   ht   hu   hv   hw   hx   hy   hz   ia   ib   ic   id   ie   if   ig 
##   14    1    1    1    3    2    9    6    3    3    1    1    3    2    1    1 
##   ih   ii   ij   ik   il   im   in   io   ip   iq   ir   it   iu   iv   iw   ix 
##    1    2    5    1    1    3    4    2    2    3    3    3    1    2    3    1 
##   iy   iz   ja   jb   jc   jd   je   jf   jg   ji   jj   jk   jl   jm   jn   jp 
##    1    2    1    1    2    1    1    1    1    1    1    1    1    1    1    1 
##   jq   jr   js   jt   ju   jv   jw   jy   ka   kb   kc   kh   kj   kk   km   kn 
##    6    8   30   36    8   11   13   67    5    1    1    4    3    2    5    4 
##   ko   kp   kq   kr   ks   kt   ku   kv   kw   kx   ky   kz   la   lb   lc   ld 
##    4   10    9    2    3    4   22    3    4    6    7    1    2    9    1    8 
##   le   lf   lg   lh   li   lj   lk   ll   lm   ln   lo   lp   lq   lr   ls   lt 
##    4   24   27    2    2    1    1    2    5   12    3    5    3    4    9    1 
##   lu   lw   lx   ly   lz   ma   mb   mc   me   mf   mg   mh   mi   mj   mk   ml 
##    1    1    9    3    3    3    3    4    6    6   10    1    2    5   16    4 
##   mm   mn   mo   mp   mq   ms   mt   mv   mw   my   mz   na   nb   nc   nd   ne 
##    2    3    2    6    2    2    8   19    3    1    1    9    3    2    7    2 
##   nf   ng   nh   ni   nj   nk   nl   nm   nn   no   np   nq   nr   ns   nt   nu 
##    3    3    3    5    4    4    2    3    8    1    3    1    4    9    3    2 
##   nv   nw   nx   ny   nz   oa   ob   oc   od   oe   of   og   oh   oi   oj   ok 
##    2    2   10    3    1    2    2    1    4   12   10    1    1    2    2    1 
##   ol   om   on   oo   op   oq   or   os   ot   ou   ov   ow   ox   oy   oz   pa 
##    6    1    2    4    1    1    7    3    2    5    2   14    2    3    1    1 
##   pb   pc   pd   pe   pf   pg   ph   pi   pj   pk   pl   pm   pn   po   pp   pq 
##    1    1    1    3    1    2    9    4    1    1    6    3    1    3    1    1 
##   pr   ps   pt   pv   pw   py   pz   qb   qc   qd   qe   qf   qg   qh   qj   qk 
##    1    1    3    5    2    1    1    1    1    2    3    1    1    3    2    3 
##   ql   qm   qn   qo   qp   qq   qr   qs   qt   qu   qv   qx   qy   qz   ra   rb 
##    1    1    2    2    2    2    4    2    3    1    1    1    1    1    2    5 
##   rc   re   rf   rg   rh   ri   rj   rk   rl   rn   ro   rp   rq   rr   rs   rt 
##    2    3    1    1    1    1    1    1    2    1    2    1    1    1    1    1 
##   ru   rv   rw   rx   ry   rz   sa   sb   sc   sd   se   sf   sg   sh   sj   sk 
##    1    1    1    1    1    1    2    1    1    1    1    1    1    1    1    1 
##   sl   sm   sn   so   sp   sq   sr   st   su 
##    1    1    1    1    1    1    1    1    1
# ab, es, ff etc all refer to specific nodes int he tree taxonomic groups. the number referts to how many sequences belong to that taxonomy (all 1000 sequences, are part of Bacteria "ab", 574 are Proteobacteria "ac")
taxmap_obj$taxa[1:10]
## $ab
## <Taxon>
##   name: Bacteria
##   rank: Kingdom
##   id: none
##   authority: none
## 
## $ac
## <Taxon>
##   name: Proteobacteria
##   rank: Phylum
##   id: none
##   authority: none
## 
## $ad
## <Taxon>
##   name: Bacteroidota
##   rank: Phylum
##   id: none
##   authority: none
## 
## $ae
## <Taxon>
##   name: Actinobacteriota
##   rank: Phylum
##   id: none
##   authority: none
## 
## $af
## <Taxon>
##   name: Acidobacteriota
##   rank: Phylum
##   id: none
##   authority: none
## 
## $ag
## <Taxon>
##   name: Gemmatimonadota
##   rank: Phylum
##   id: none
##   authority: none
## 
## $ah
## <Taxon>
##   name: Myxococcota
##   rank: Phylum
##   id: none
##   authority: none
## 
## $ai
## <Taxon>
##   name: Firmicutes
##   rank: Phylum
##   id: none
##   authority: none
## 
## $aj
## <Taxon>
##   name: Methylomirabilota
##   rank: Phylum
##   id: none
##   authority: none
## 
## $ak
## <Taxon>
##   name: Armatimonadota
##   rank: Phylum
##   id: none
##   authority: none

so if you have a vector from the same lenght of n_obs(taxmap_obj) with data from other analysis, you can visualize other outputs in a heat tree! lets prepare a df that will add new, random data

5.2 - extract taon infor from the heatmap object

let’s extract the taxon ides from the metacoder object

then create new (random) data, and add this random data to the heat tree

#extract the names of the taxonomies in the heat tree, accoding the ab ac etc order
taxon_id_metacoder<-lapply(taxmap_obj$taxa, function (x)
                                                    x$get_name())%>%
                                                    map(1)

# now turn that list into a df
taxon_id_metacoder<-do.call(rbind.data.frame, map(taxon_id_metacoder,1))

# and change column name
colnames(taxon_id_metacoder)<-"taxa_id"

#check output
head(taxon_id_metacoder)
##            taxa_id
## 1         Bacteria
## 2   Proteobacteria
## 3     Bacteroidota
## 4 Actinobacteriota
## 5  Acidobacteriota
## 6  Gemmatimonadota

5.3 - check some input arguments of a heat tree

then create new (random) data, adn add it to the taxon information you extrated

#make up some random data called new_data1
set.seed(1)
new_data1<-c(runif(n=100, min=1, max=10),
            runif(n=200, min=5, max=15),
            runif(n=100, min=10, max=20),
            runif(n=57, min=20, max=25))

#make up some random data called new_data2
set.seed(1)
new_data2<-c(runif(n=57, min=1000, max=2000),
             runif(n=200, min=600, max=1000),
             runif(n=200, min=200, max=500))


# add new (random example) data into the df
taxon_id_metacoder$new_analysis_output1<-new_data1
taxon_id_metacoder$new_analysis_output2<-new_data2

#this is the df we created using data external from metacoder
taxon_id_metacoder[1:10,]
##              taxa_id new_analysis_output1 new_analysis_output2
## 1           Bacteria             3.389578             1265.509
## 2     Proteobacteria             4.349115             1372.124
## 3       Bacteroidota             6.155680             1572.853
## 4   Actinobacteriota             9.173870             1908.208
## 5    Acidobacteriota             2.815137             1201.682
## 6    Gemmatimonadota             9.085507             1898.390
## 7        Myxococcota             9.502077             1944.675
## 8         Firmicutes             6.947180             1660.798
## 9  Methylomirabilota             6.662026             1629.114
## 10    Armatimonadota             1.556076             1061.786

5.4 - make a new heat tree with the enw random data

the size of the node will relate to the first column of the new dataframe. the color of the node will related the the second column of the new dataframe

in a real case, you would have your valuable data, taxon by taxon, in a dataframe with simialr structure

# add the new data into the heat tree!
taxmap_obj %>%
  heat_tree(node_label = taxon_names,
            node_size = taxon_id_metacoder$new_analysis_output1,
            node_color = taxon_id_metacoder$new_analysis_output2,
            node_size_axis_label = "Size: new data 2",
            node_color_axis_label = "Color: new data 1",
            layout = "davidson-harel",
            initial_layout = "reingold-tilford")

Of course, there are countless ways you can integrate different pieces of data together - such as by matching the taxonomies ab, ac, ad etc; the names of the taxa like “f__Burkholderiales” etc. Note that you won’t find sample code for this - adding external data - in the metacoder package documentation.

6.0 - adding results of a fisher tests to a heat tree

In the MeJA applications pilot were we tested different MeJA concentrations, we run different analysis that highlight taxa as “important”: predictors for stress in random forest, keystone nodes in networks, and differentially abudnant ASVs in treatment-control comparisons. to select which of the taxonomies of these “important” taxa to focus on, we performed a fisher test to check if the proportions of a taxonomy are similar between the importnat ASVs and the ASVs that were not defined as important

6.1 - load important taxa names, create new ps object

these ASVs listed in “imp_asv_list.Rdata” were highlighted as differential abundant due to stress OR important in random forest predictions for stress OR network keystone/connector/hub of the species in a more complete dataset. I call these the “important”ASVs.

after we load these ASVs anmes, we create a list of new ps objects by running phyloseq::prune_taxa (a funcntion that selects taxa in a ps object according a character vector) with base::mapply (a function that runs a function on two lists simultaneously)

# load a list of ASV names. 
load( file="./data/imp_asv_list.Rdata")

imp_asv_list # as you see, this is just a list of taxa
## $At
##   [1] "bASV_9"     "bASV_29"    "bASV_71"    "bASV_97"    "bASV_108"  
##   [6] "bASV_155"   "bASV_169"   "bASV_175"   "bASV_314"   "bASV_391"  
##  [11] "bASV_443"   "bASV_674"   "bASV_776"   "bASV_1058"  "bASV_1085" 
##  [16] "bASV_1526"  "bASV_1722"  "bASV_2147"  "bASV_4416"  "bASV_1008" 
##  [21] "bASV_1035"  "bASV_105"   "bASV_1054"  "bASV_106"   "bASV_111"  
##  [26] "bASV_115"   "bASV_117"   "bASV_119"   "bASV_12"    "bASV_120"  
##  [31] "bASV_1244"  "bASV_13"    "bASV_132"   "bASV_133"   "bASV_134"  
##  [36] "bASV_138"   "bASV_1400"  "bASV_1421"  "bASV_143"   "bASV_154"  
##  [41] "bASV_158"   "bASV_162"   "bASV_165"   "bASV_166"   "bASV_190"  
##  [46] "bASV_192"   "bASV_200"   "bASV_202"   "bASV_205"   "bASV_21"   
##  [51] "bASV_218"   "bASV_224"   "bASV_226"   "bASV_233"   "bASV_234"  
##  [56] "bASV_254"   "bASV_274"   "bASV_279"   "bASV_280"   "bASV_289"  
##  [61] "bASV_292"   "bASV_30"    "bASV_31"    "bASV_319"   "bASV_32"   
##  [66] "bASV_333"   "bASV_337"   "bASV_339"   "bASV_347"   "bASV_36"   
##  [71] "bASV_369"   "bASV_37"    "bASV_38"    "bASV_39"    "bASV_395"  
##  [76] "bASV_40"    "bASV_406"   "bASV_424"   "bASV_4245"  "bASV_428"  
##  [81] "bASV_439"   "bASV_442"   "bASV_458"   "bASV_46"    "bASV_48"   
##  [86] "bASV_493"   "bASV_50"    "bASV_514"   "bASV_515"   "bASV_517"  
##  [91] "bASV_530"   "bASV_531"   "bASV_557"   "bASV_573"   "bASV_58"   
##  [96] "bASV_59"    "bASV_599"   "bASV_60"    "bASV_605"   "bASV_61"   
## [101] "bASV_622"   "bASV_63"    "bASV_638"   "bASV_64"    "bASV_65"   
## [106] "bASV_66"    "bASV_698"   "bASV_7"     "bASV_709"   "bASV_75"   
## [111] "bASV_76"    "bASV_762"   "bASV_81"    "bASV_83"    "bASV_878"  
## [116] "bASV_892"   "bASV_90"    "bASV_964"   "bASV_98"    "bASV_99"   
## [121] "bASV_153"   "bASV_845"   "bASV_1590"  "bASV_2049"  "bASV_889"  
## [126] "bASV_6729"  "bASV_10033" "bASV_11577" "bASV_12011" "bASV_22991"
## [131] "bASV_24722" "bASV_27301" "bASV_35730" "bASV_38129" "bASV_67851"
## [136] "bASV_28909" "bASV_7928" 
## 
## $Bo_M
##   [1] "bASV_18"   "bASV_29"   "bASV_53"   "bASV_66"   "bASV_67"   "bASV_85"  
##   [7] "bASV_143"  "bASV_226"  "bASV_286"  "bASV_291"  "bASV_339"  "bASV_417" 
##  [13] "bASV_418"  "bASV_550"  "bASV_814"  "bASV_999"  "bASV_1018" "bASV_1238"
##  [19] "bASV_1305" "bASV_1464" "bASV_2760" "bASV_2999" "bASV_3347" "bASV_3617"
##  [25] "bASV_102"  "bASV_104"  "bASV_106"  "bASV_110"  "bASV_112"  "bASV_115" 
##  [31] "bASV_118"  "bASV_12"   "bASV_123"  "bASV_1245" "bASV_13"   "bASV_132" 
##  [37] "bASV_134"  "bASV_1346" "bASV_137"  "bASV_139"  "bASV_145"  "bASV_146" 
##  [43] "bASV_15"   "bASV_164"  "bASV_166"  "bASV_170"  "bASV_174"  "bASV_181" 
##  [49] "bASV_195"  "bASV_196"  "bASV_203"  "bASV_209"  "bASV_214"  "bASV_223" 
##  [55] "bASV_230"  "bASV_244"  "bASV_251"  "bASV_255"  "bASV_261"  "bASV_266" 
##  [61] "bASV_282"  "bASV_309"  "bASV_310"  "bASV_321"  "bASV_326"  "bASV_345" 
##  [67] "bASV_349"  "bASV_355"  "bASV_358"  "bASV_376"  "bASV_404"  "bASV_439" 
##  [73] "bASV_45"   "bASV_474"  "bASV_48"   "bASV_481"  "bASV_542"  "bASV_55"  
##  [79] "bASV_58"   "bASV_590"  "bASV_591"  "bASV_613"  "bASV_62"   "bASV_64"  
##  [85] "bASV_73"   "bASV_731"  "bASV_754"  "bASV_763"  "bASV_780"  "bASV_793" 
##  [91] "bASV_839"  "bASV_91"   "bASV_97"   "bASV_98"   "bASV_136"  "bASV_320" 
##  [97] "bASV_1185" "bASV_278"  "bASV_594"  "bASV_1112" "bASV_316"  "bASV_664" 
## [103] "bASV_775" 
## 
## $It
##   [1] "bASV_11"    "bASV_46"    "bASV_54"    "bASV_55"    "bASV_58"   
##   [6] "bASV_85"    "bASV_102"   "bASV_109"   "bASV_129"   "bASV_190"  
##  [11] "bASV_216"   "bASV_223"   "bASV_259"   "bASV_314"   "bASV_330"  
##  [16] "bASV_400"   "bASV_520"   "bASV_662"   "bASV_716"   "bASV_729"  
##  [21] "bASV_766"   "bASV_776"   "bASV_838"   "bASV_1134"  "bASV_1232" 
##  [26] "bASV_1469"  "bASV_1846"  "bASV_2793"  "bASV_1019"  "bASV_1039" 
##  [31] "bASV_111"   "bASV_116"   "bASV_12"    "bASV_1228"  "bASV_124"  
##  [36] "bASV_1242"  "bASV_142"   "bASV_149"   "bASV_15"    "bASV_1532" 
##  [41] "bASV_154"   "bASV_160"   "bASV_161"   "bASV_162"   "bASV_175"  
##  [46] "bASV_180"   "bASV_182"   "bASV_1867"  "bASV_187"   "bASV_1959" 
##  [51] "bASV_20"    "bASV_207"   "bASV_21"    "bASV_215"   "bASV_217"  
##  [56] "bASV_219"   "bASV_230"   "bASV_246"   "bASV_249"   "bASV_25"   
##  [61] "bASV_261"   "bASV_270"   "bASV_274"   "bASV_277"   "bASV_280"  
##  [66] "bASV_312"   "bASV_32"    "bASV_323"   "bASV_350"   "bASV_362"  
##  [71] "bASV_369"   "bASV_372"   "bASV_384"   "bASV_392"   "bASV_40"   
##  [76] "bASV_407"   "bASV_425"   "bASV_438"   "bASV_44"    "bASV_443"  
##  [81] "bASV_45"    "bASV_451"   "bASV_476"   "bASV_477"   "bASV_496"  
##  [86] "bASV_504"   "bASV_508"   "bASV_52"    "bASV_569"   "bASV_589"  
##  [91] "bASV_590"   "bASV_593"   "bASV_601"   "bASV_602"   "bASV_62"   
##  [96] "bASV_682"   "bASV_69"    "bASV_691"   "bASV_705"   "bASV_725"  
## [101] "bASV_76"    "bASV_768"   "bASV_812"   "bASV_840"   "bASV_935"  
## [106] "bASV_946"   "bASV_210"   "bASV_1006"  "bASV_515"   "bASV_2687" 
## [111] "bASV_473"   "bASV_5751"  "bASV_4308"  "bASV_8032"  "bASV_30283"
## [116] "bASV_59443" "bASV_604"   "bASV_1093"  "bASV_394"   "bASV_1002"
# filter your list of phyloliseq objects to only contain the ASVs defined as "importat"
imp_ASV_ps_l<-mapply (function (list_1,list_2) #mapply will let you manipulate 2 or more lists at once
            prune_taxa(taxa = list_1, x = list_2), #here, x as the name of an argument of prune_taxa, and refers to a phyloseq object
          list_1 = imp_asv_list, # here you define what R object is list_1
          list_2 = ps_l, # here you define what R object is list_2
          SIMPLIFY = FALSE)

# this ps object only has "important" ASVs
imp_ASV_ps_l
## $At
## phyloseq-class experiment-level object
## otu_table()   OTU Table:         [ 114 taxa and 48 samples ]
## sample_data() Sample Data:       [ 48 samples by 44 sample variables ]
## tax_table()   Taxonomy Table:    [ 114 taxa by 8 taxonomic ranks ]
## refseq()      DNAStringSet:      [ 114 reference sequences ]
## 
## $Bo_M
## phyloseq-class experiment-level object
## otu_table()   OTU Table:         [ 92 taxa and 48 samples ]
## sample_data() Sample Data:       [ 48 samples by 44 sample variables ]
## tax_table()   Taxonomy Table:    [ 92 taxa by 8 taxonomic ranks ]
## refseq()      DNAStringSet:      [ 92 reference sequences ]
## 
## $It
## phyloseq-class experiment-level object
## otu_table()   OTU Table:         [ 102 taxa and 47 samples ]
## sample_data() Sample Data:       [ 47 samples by 44 sample variables ]
## tax_table()   Taxonomy Table:    [ 102 taxa by 8 taxonomic ranks ]
## refseq()      DNAStringSet:      [ 102 reference sequences ]

6.2 - define the funciton that calculates fisher tests and heat trees

If we have the time, we explore details for these functions. their description and comments should be fairly sufficiency

the fisher_all_taxa_groups function requires as input: 1) a phyloseq object that contains your “important” taxa 2) a phyloseq objects that contains all your taxa

the fisher_to_heatTree function requires as input 1) the output of the fisher_all_taxa_groups function 2) a phyloseq object that contains your “important” taxa

#define function that calculates proportion tests between 2 phyloseq objects
fisher_all_taxa_groups<-function(ps_important_taxa, ps_all_taxa){

#**********************************************#
############# fisher_all_taxa_groups ################
#**********************************************#

# this function takes 2 phyloseq objects as 2 arguments:
# ps_important_taxa =  a subset of imporntant taxa, such as ASVs tagged as important by differential abundance, random forest, and netowrk analysis
# ps_all_taxa = the full phyloseq objects from where you obtained the imporntat subset (and likely your input for differential abundance, random forest, and netowork analysis)

# Then it runs a fisher test, comparing the proportions of every taxonomic level accuting more than twice in both datasets
# it's just a test to compare proportions - is 6 out of 17 a similar proportion to 193 out of 2243?

  
  
# first, get the taxonomic groups of the taxa defined as important
  
  #this will get us a list of (taxa_level) that appears in the important subset more than once
  imp_phylum_l<-as.character(tax_table(ps_important_taxa)[,"Phylum"]) # gets a char vector  of "families" shown as relevant
  imp_phylum_l<-imp_phylum_l[imp_phylum_l != "p__uncultured"] # removes any taxonomy set as "uncultured"
  imp_phylum_l<-names(which(table(imp_phylum_l)>2))%>% # get names of taxa that occur ate least 1 time NOTE: CHANGED FROM >2
    na.omit()%>% #remove NA from classifications
    unique()%>%  #dereplicates repetitive values (avoids "f__Chitinophagaceae" "f__Chitinophagaceae" "f__Oxalobacteraceae")
    as.list(c()) # save the dereplicated values as a list
  
  # now, perfom the same as above for every taxa level... it's hard-coded, but it works well enough. Pedro tried automating this and gave up after a few hours
  imp_class_l<-as.character(tax_table(ps_important_taxa)[,"Class"]) 
  imp_class_l<-imp_class_l[imp_class_l != "c__uncultured"] 
  imp_class_l<-names(which(table(imp_class_l)>2))%>% 
    na.omit()%>% 
    unique()%>%  
    as.list(c()) 
  
  imp_order_l<-as.character(tax_table(ps_important_taxa)[,"Order"])
  imp_order_l<-imp_order_l[imp_order_l != "o__uncultured"]
  imp_order_l<-names(which(table(imp_order_l)>2))%>%
    na.omit()%>% 
    unique()%>%
    as.list(c()) 
  
  imp_fam_l<-as.character(tax_table(ps_important_taxa)[,"Family"])
  imp_fam_l<-imp_fam_l[imp_fam_l != "f__uncultured"]
  imp_fam_l<-names(which(table(imp_fam_l)>2))%>%
    na.omit()%>% 
    unique()%>% 
    as.list(c()) 
  
  imp_genus_l<-as.character(tax_table(ps_important_taxa)[,"Genus"])
  imp_genus_l<-imp_genus_l[imp_genus_l != "g__uncultured"]
  imp_genus_l<-names(which(table(imp_genus_l)>2))%>%
    na.omit()%>% 
    unique()%>% 
    as.list(c()) 
  
  
  
  
  
  
  
  
  
  # second, get the number of taxa occuring in each taxonomic group, within the imporntat taxa subset
  # note: using phyloseq::subset_taxa with a for loop will cause issues as your taxa is intepreted as "i"! to avoid this, use phyloseq::prune_taxa instead
  
  
  
  #make one empty lists to store results
  target_in_important_n<-list()
  
  # obtain the number of reads of the target taxa in the important subset
  for(i in imp_phylum_l) { #for every Phylum "i" with representatives defined as important....
    target_in_important_n[i]<-phyloseq::ntaxa(prune_taxa(taxa = tax_table(ps_important_taxa)[,"Phylum"] %in% i, # get only the taxa that belong to that particular phylum "i", then define the number of taxa in there, then save it in the empty list we just made to store the results
                                                         x = ps_important_taxa))
  }
  
  # now, perfom the same as above for every taxa level... it's hard-coded, but it works well enough. Pedro tried automating this and gave up after a few hours
  for(i in imp_class_l) {
    target_in_important_n[i]<-phyloseq::ntaxa(prune_taxa(taxa = tax_table(ps_important_taxa)[,"Class"] %in% i,
                                                         x = ps_important_taxa))
  }
  
  for(i in imp_order_l) {
    target_in_important_n[i]<-phyloseq::ntaxa(prune_taxa(taxa = tax_table(ps_important_taxa)[,"Order"] %in% i,
                                                         x = ps_important_taxa))
  }
  
  
  for(i in imp_fam_l) {
    target_in_important_n[i]<-phyloseq::ntaxa(prune_taxa(taxa = tax_table(ps_important_taxa)[,"Family"] %in% i,
                                                         x = ps_important_taxa))
  }
  
  for(i in imp_genus_l) {
    target_in_important_n[i]<-phyloseq::ntaxa(prune_taxa(taxa = tax_table(ps_important_taxa)[,"Genus"] %in% i,
                                                         x = ps_important_taxa))
  }
  
  
  
  
  
  
  
  
  
  # third, get the number of taxa occuring in each taxonomic group, within the full dataset 
  
  #make one empty lists to store results
  target_in_all_n<-list()
  
  # obtain the number of reads of the target taxa in the important subset
  for(i in imp_phylum_l) { #for every Phylum "i" with representatives defined as important....
    target_in_all_n[i]<-phyloseq::ntaxa(prune_taxa(taxa = tax_table(ps_all_taxa)[,"Phylum"] %in% i, # get only the taxa that belong to that particular phylum "i", then define the number of taxa in there, then save it in the empty list we just made to store the results
                                                   x = ps_all_taxa))
  }
  
  # now, perfom the same as above for every taxa level... it's hard-coded, but it works well enough. Pedro tried automating this and gave up after a few hours
  for(i in imp_class_l) {
    target_in_all_n[i]<-phyloseq::ntaxa(prune_taxa(taxa = tax_table(ps_all_taxa)[,"Class"] %in% i,
                                                   x = ps_all_taxa))
  }
  
  for(i in imp_order_l) {
    target_in_all_n[i]<-phyloseq::ntaxa(prune_taxa(taxa = tax_table(ps_all_taxa)[,"Order"] %in% i,
                                                   x = ps_all_taxa))
  }
  
  
  for(i in imp_fam_l) {
    target_in_all_n[i]<-phyloseq::ntaxa(prune_taxa(taxa = tax_table(ps_all_taxa)[,"Family"] %in% i,
                                                   x = ps_all_taxa))
  }
  
  for(i in imp_genus_l) {
    target_in_all_n[i]<-phyloseq::ntaxa(prune_taxa(taxa = tax_table(ps_all_taxa)[,"Genus"] %in% i,
                                                   x = ps_all_taxa))
  }
  
  
  
  
  
  
  
  
  
  # now, get the total number of taxa in the imporntat subset and in the full dataset
  
  # all important taxa
  all_taxa_in_important_n<-phyloseq::ntaxa(ps_important_taxa)
  
  #all taxa 
  all_taxa_in_all_n<-phyloseq::ntaxa(ps_all_taxa)
  
  
  
  
  
  
  
  
  
  # now perform fisher tests over lists; check online tutorials for fisher.test() if need
  
  # this contigency table: (summed marginal totals is equal to the total number of taxa the the full object)
  fisher_result<-mapply(function (target_in_important_n,target_in_all_n)
    fisher.test(matrix(c(target_in_important_n, 
                         all_taxa_in_important_n - target_in_important_n, 
                         target_in_all_n - target_in_important_n, 
                         all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n),
                       ncol=2),alternative = "greater" ), 
    target_in_all_n = target_in_all_n,
    target_in_important_n = target_in_important_n,
    SIMPLIFY = FALSE)
  
  return(fisher_result)
  
}

#define function that puts fisher tests in metacoder heat trees
fisher_to_heatTree<-function(fisher_output, ps_important_taxa){
#**********************************************#
############# fisher_to_heatTree ################
#**********************************************#
  
# this function takes 1 phyloseq object and the putput of the fisher_all_taxa_groups() function as 2 arguments:
# fisher_output =  The output of the function defined above, fisher_all_taxa_groups
# ps_important_taxa = a phtloseq object with the taxa classified as "important". it is the same input as you used in the function fisher_all_taxa_groups  

# this function will:
  # 1)  create a taxmap object from the phyloseq object
  # 2)  extract the p values from the fisher test and apply an fdr correction
  # 3)  extract the taxa names form the heat tree, and match it to the p values of the fisher test
  # 4)  extract the odds ratio from the fisher test and add it to the extracted p values adn taxonomies
  # 5)  creates a heat map of the important taxa, with colour for adjusted p values and node size for odds ratio

  
  
#######################################
######## make metacoder object ######## 
#######################################
imp_heat_ps<-ps_important_taxa

#remove unecessary taxonomic indo (dada2id, "S__" and" above_selected)
tax_table(imp_heat_ps)<-tax_table(imp_heat_ps)[,1:6]

# let's remove the "r__"ranks from the taxonomy, they can be useful but will pollute our plot
#tax_table(imp_heat_ps)[, colnames(tax_table(imp_heat_ps))] <- gsub(pattern = "[a-z]__", # regular expression pattern to search for
 #                                                                  x = tax_table(imp_heat_ps)[, colnames(tax_table(imp_heat_ps))], # "df"
  #                                                                 replacement = "") # replacement for pattern

# transform from phyloseq to  taxmap object
imp_heat<-parse_phyloseq(imp_heat_ps)



#######################################
######## aplly fdr to fisher ######## 
#######################################

#apply FDR correction to the p values

  #single df with all p values
  pvector<-do.call(rbind.data.frame, map(fisher_output,1))
  
  # adjust the p value with fdr, then save as a list
  adjusted_p<-p.adjust(p = pvector[[1]],
                          method = "fdr")%>%
                      as.list()
  # adjust names
  names(adjusted_p)<-names(fisher_output)

  

#this is the name that mustch match our fisher p list
taxon_id_metacoder<-lapply(imp_heat$taxa, function (x)
                                                    x$get_name())%>%
                                                    map(1)


# now turn that list into a df
taxon_id_metacoder<-do.call(rbind.data.frame, map(taxon_id_metacoder,1))

# and change column name
colnames(taxon_id_metacoder)<-"taxa_id"

# truns the p values into a dataframe
adjusted_p_input<-as.data.frame(t(as.data.frame(adjusted_p)))%>%
  rownames_to_column()

#change column names
colnames(adjusted_p_input)<-c("taxa_id", "fdr_p")
  
# now join both df
fdr_for_heatTree<-left_join(taxon_id_metacoder,
                            adjusted_p_input,
                            by = "taxa_id")



#######################################################################
################### add fold changes! ########################################
#######################################################################


# get odds ratio for each taxa
odd_list<- map(fisher_output,3)%>%
  map(1)

# truns the p values into a dataframe
odd_list<-as.data.frame(t(as.data.frame(odd_list)))%>%
  rownames_to_column()

#change column names
colnames(odd_list)<-c("taxa_id", "odds_ratio")

# now join both df
fisher_for_heatTree<-left_join(fdr_for_heatTree,
                            odd_list,
                            by = "taxa_id")




#NAs will crash metadore. let's change them with 0.9999 for now
fisher_for_heatTree[is.na(fisher_for_heatTree)]<-"1"

#change structure to numeric
fisher_for_heatTree$fdr_p<-as.numeric(fisher_for_heatTree$fdr_p)
fisher_for_heatTree$odds_ratio<-as.numeric(fisher_for_heatTree$odds_ratio)


#now let's dim p values above 0.1, or else we can get counfounded
#fisher_for_heatTree$fdr_p[fisher_for_heatTree$fdr_p>0.1]<-1







#######################################################################
############### now plot the heat tree ############### 
#######################################################################



set.seed(1)
      output<- heat_tree(imp_heat,
                 node_size = fisher_for_heatTree$odds_ratio, # n_obs is a function that calculates, in this case, the number of OTUs per taxon
                 node_color = fisher_for_heatTree$fdr_p,
                 node_label = taxon_names,
                 node_size_axis_label = "Size: oods_ratio",
                 node_color_interval = c(0, 0.15),
                 edge_color_interval = c(0, 0.15),
                 node_color_range = c("darkgreen", "seagreen", "yellowgreen", "grey"),
                 edge_color_range = c("darkgreen", "seagreen", "yellowgreen", "grey"),
                 node_color_axis_label = "Color: FDR-adjusted p",
                 layout = "davidson-harel", # The primary layout algorithm
                 initial_layout = "reingold-tilford") # The layout algorithm that initializes node locations

      return(output)
}

6.3 - check some of the proportions we will test

is a specific taxonomy overly frequent in the “important” dataset when compared to the taxonomies of the ASV that were NOT defined as important?

note that the phyloseq input is based on the original phyloseq object, that still contains the ranks as p__Proteobacteria, o__Rhizobiales, etc

# how many rhizobiales do we have in full set of 963 ASVs of A. thaliana?
ntaxa(physeq = subset_taxa(ps_l$At, Order == "o__Rhizobiales"))
## [1] 81
# how many rhizobiales do we have in the group of 114 "important" ASVs for A. thaliana?
ntaxa(physeq = subset_taxa(imp_ASV_ps_l$At, Order == "o__Rhizobiales"))
## [1] 18
# how many rhizobiales do we have in the set of 849 A. thaliana ASVs that were not tagged as important ?
unimp_ASV_ps_at<-prune_taxa(!(taxa_names(ps_l$At) %in% imp_asv_list$At), ps_l$At)
ntaxa(physeq = subset_taxa(unimp_ASV_ps_at, Order == "o__Rhizobiales"))
## [1] 63

is 18 in 114 (~15%) similar to 63 in 849 (~7%), considering we have in total 18+63 (81) rhizobiales in 114+849 (983) bASVs? if these proportions are statistically different, rhizobiales may be overrepresented in the important ASV set

6.4 - calculate prortions of the fisher test

by running the functions defined in chunk #6.2, we test the proportions stated in chunk # 6.3 - on all taxonomic levels of your input phyloseq object

# run a fisher test comparin these proportions at all taxonomic levels
fisher_output_at<-fisher_all_taxa_groups(ps_important_taxa = imp_ASV_ps_l$At,
                                         ps_all_taxa = ps_l$At)

#check raw ouput of fisher tests for rhizobiales
fisher_output_at
## $p__Acidobacteriota
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.8474
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.3153814       Inf
## sample estimates:
## odds ratio 
##  0.7106417 
## 
## 
## $p__Actinobacteriota
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.9985
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.06810393        Inf
## sample estimates:
## odds ratio 
##  0.2590167 
## 
## 
## $p__Bacteroidota
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.09193
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.9037439       Inf
## sample estimates:
## odds ratio 
##   1.557417 
## 
## 
## $p__Gemmatimonadota
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.8433
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.254939      Inf
## sample estimates:
## odds ratio 
##   0.684851 
## 
## 
## $p__Myxococcota
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.09168
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.7972862       Inf
## sample estimates:
## odds ratio 
##   2.752893 
## 
## 
## $p__Proteobacteria
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.1964
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.8497712       Inf
## sample estimates:
## odds ratio 
##   1.223502 
## 
## 
## $c__Acidobacteriae
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.4959
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.2916697       Inf
## sample estimates:
## odds ratio 
##   1.176164 
## 
## 
## $c__Alphaproteobacteria
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.3283
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.745213      Inf
## sample estimates:
## odds ratio 
##   1.138922 
## 
## 
## $c__Bacteroidia
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.09193
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.9037439       Inf
## sample estimates:
## odds ratio 
##   1.557417 
## 
## 
## $c__Gammaproteobacteria
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.1752
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.8638619       Inf
## sample estimates:
## odds ratio 
##   1.233855 
## 
## 
## $c__Gemmatimonadetes
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.8197
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.265233      Inf
## sample estimates:
## odds ratio 
##  0.7134832 
## 
## 
## $c__Polyangia
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.05783
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.9483557       Inf
## sample estimates:
## odds ratio 
##    3.37105 
## 
## 
## $o__Burkholderiales
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.5466
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.6699675       Inf
## sample estimates:
## odds ratio 
##  0.9959746 
## 
## 
## $o__Caulobacterales
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.305
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.518229      Inf
## sample estimates:
## odds ratio 
##   1.442557 
## 
## 
## $o__Chitinophagales
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.2736
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.6284454       Inf
## sample estimates:
## odds ratio 
##   1.366568 
## 
## 
## $o__Cytophagales
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.2594
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.4460974       Inf
## sample estimates:
## odds ratio 
##   1.876752 
## 
## 
## $o__Flavobacteriales
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.05783
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.9483557       Inf
## sample estimates:
## odds ratio 
##    3.37105 
## 
## 
## $o__Gammaproteobacteria_Incertae_Sedis
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.05843
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.9328861       Inf
## sample estimates:
## odds ratio 
##   4.534428 
## 
## 
## $o__Gemmatimonadales
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.8197
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.265233      Inf
## sample estimates:
## odds ratio 
##  0.7134832 
## 
## 
## $o__Rhizobiales
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.004991
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  1.345852      Inf
## sample estimates:
## odds ratio 
##   2.283077 
## 
## 
## $o__Sphingomonadales
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.9913
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.08653077        Inf
## sample estimates:
## odds ratio 
##  0.3304971 
## 
## 
## $o__Xanthomonadales
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.09127
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.8852716       Inf
## sample estimates:
## odds ratio 
##   1.718467 
## 
## 
## $f__Caulobacteraceae
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.2804
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.5381227       Inf
## sample estimates:
## odds ratio 
##    1.50201 
## 
## 
## $f__Chitinophagaceae
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.2555
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.6430935       Inf
## sample estimates:
## odds ratio 
##   1.400058 
## 
## 
## $f__Comamonadaceae
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.0723
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.9435397       Inf
## sample estimates:
## odds ratio 
##   1.602046 
## 
## 
## $f__Devosiaceae
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.005974
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  2.480493      Inf
## sample estimates:
## odds ratio 
##   22.69165 
## 
## 
## $f__Gemmatimonadaceae
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.8197
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.265233      Inf
## sample estimates:
## odds ratio 
##  0.7134832 
## 
## 
## $f__Oxalobacteraceae
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.3632
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.6540035       Inf
## sample estimates:
## odds ratio 
##   1.158663 
## 
## 
## $f__Rhizobiaceae
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.05559
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.9708741       Inf
## sample estimates:
## odds ratio 
##   2.542972 
## 
## 
## $f__Rhodanobacteraceae
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.3626
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.3639173       Inf
## sample estimates:
## odds ratio 
##   1.496563 
## 
## 
## $f__Sphingomonadaceae
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.9913
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.08653077        Inf
## sample estimates:
## odds ratio 
##  0.3304971 
## 
## 
## $f__Unknown_Family
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.05843
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.9328861       Inf
## sample estimates:
## odds ratio 
##   4.534428 
## 
## 
## $f__Xanthobacteraceae
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.12
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.7725396       Inf
## sample estimates:
## odds ratio 
##   1.978932 
## 
## 
## $f__Xanthomonadaceae
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.1163
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.8112345       Inf
## sample estimates:
## odds ratio 
##   1.790011 
## 
## 
## $g__Acidibacter
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.05843
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.9328861       Inf
## sample estimates:
## odds ratio 
##   4.534428 
## 
## 
## $`g__Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium`
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.1324
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.636317      Inf
## sample estimates:
## odds ratio 
##   2.826685 
## 
## 
## $g__Gemmatimonas
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.374
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.4222894       Inf
## sample estimates:
## odds ratio 
##    1.35984 
## 
## 
## $g__Lysobacter
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.1097
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.7730246       Inf
## sample estimates:
## odds ratio 
##   2.229048 
## 
## 
## $g__Massilia
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.3344
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  0.6047344       Inf
## sample estimates:
## odds ratio 
##   1.247307 
## 
## 
## $g__Niastella
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.0196
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  1.229124      Inf
## sample estimates:
## odds ratio 
##   2.805325
fisher_output_at$o__Rhizobiales
## 
##  Fisher's Exact Test for Count Data
## 
## data:  matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_important_n, all_taxa_in_all_n - all_taxa_in_important_n - target_in_all_n), ncol = 2)
## p-value = 0.004991
## alternative hypothesis: true odds ratio is greater than 1
## 95 percent confidence interval:
##  1.345852      Inf
## sample estimates:
## odds ratio 
##   2.283077
# with map() we can easely extract key values from this output, like so:
str(fisher_output_at$o__Rhizobiales) # here we see that p values are the first list element of each taxa ouput
## List of 7
##  $ p.value    : num 0.00499
##  $ conf.int   : num [1:2] 1.35 Inf
##   ..- attr(*, "conf.level")= num 0.95
##  $ estimate   : Named num 2.28
##   ..- attr(*, "names")= chr "odds ratio"
##  $ null.value : Named num 1
##   ..- attr(*, "names")= chr "odds ratio"
##  $ alternative: chr "greater"
##  $ method     : chr "Fisher's Exact Test for Count Data"
##  $ data.name  : chr "matrix(c(target_in_important_n, all_taxa_in_important_n - target_in_important_n, target_in_all_n - target_in_im"| __truncated__
##  - attr(*, "class")= chr "htest"
map(fisher_output_at, 1) # here we extarct the first list element of each taxa in the list (p values)
## $p__Acidobacteriota
## [1] 0.8473672
## 
## $p__Actinobacteriota
## [1] 0.9985258
## 
## $p__Bacteroidota
## [1] 0.09193391
## 
## $p__Gemmatimonadota
## [1] 0.8433388
## 
## $p__Myxococcota
## [1] 0.09167539
## 
## $p__Proteobacteria
## [1] 0.1964258
## 
## $c__Acidobacteriae
## [1] 0.4959192
## 
## $c__Alphaproteobacteria
## [1] 0.3283449
## 
## $c__Bacteroidia
## [1] 0.09193391
## 
## $c__Gammaproteobacteria
## [1] 0.1752416
## 
## $c__Gemmatimonadetes
## [1] 0.8197096
## 
## $c__Polyangia
## [1] 0.0578331
## 
## $o__Burkholderiales
## [1] 0.5466162
## 
## $o__Caulobacterales
## [1] 0.3049718
## 
## $o__Chitinophagales
## [1] 0.2735582
## 
## $o__Cytophagales
## [1] 0.2593899
## 
## $o__Flavobacteriales
## [1] 0.0578331
## 
## $o__Gammaproteobacteria_Incertae_Sedis
## [1] 0.0584339
## 
## $o__Gemmatimonadales
## [1] 0.8197096
## 
## $o__Rhizobiales
## [1] 0.004991002
## 
## $o__Sphingomonadales
## [1] 0.9912935
## 
## $o__Xanthomonadales
## [1] 0.09126703
## 
## $f__Caulobacteraceae
## [1] 0.2803993
## 
## $f__Chitinophagaceae
## [1] 0.2554614
## 
## $f__Comamonadaceae
## [1] 0.07230109
## 
## $f__Devosiaceae
## [1] 0.00597422
## 
## $f__Gemmatimonadaceae
## [1] 0.8197096
## 
## $f__Oxalobacteraceae
## [1] 0.3631943
## 
## $f__Rhizobiaceae
## [1] 0.05558943
## 
## $f__Rhodanobacteraceae
## [1] 0.3626101
## 
## $f__Sphingomonadaceae
## [1] 0.9912935
## 
## $f__Unknown_Family
## [1] 0.0584339
## 
## $f__Xanthobacteraceae
## [1] 0.1200347
## 
## $f__Xanthomonadaceae
## [1] 0.1163108
## 
## $g__Acidibacter
## [1] 0.0584339
## 
## $`g__Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium`
## [1] 0.1323728
## 
## $g__Gemmatimonas
## [1] 0.3740159
## 
## $g__Lysobacter
## [1] 0.1097332
## 
## $g__Massilia
## [1] 0.3344271
## 
## $g__Niastella
## [1] 0.01960319
map(fisher_output_at, 3) # here we extarct the third list element of each taxa in the list (odds ratio)
## $p__Acidobacteriota
## odds ratio 
##  0.7106417 
## 
## $p__Actinobacteriota
## odds ratio 
##  0.2590167 
## 
## $p__Bacteroidota
## odds ratio 
##   1.557417 
## 
## $p__Gemmatimonadota
## odds ratio 
##   0.684851 
## 
## $p__Myxococcota
## odds ratio 
##   2.752893 
## 
## $p__Proteobacteria
## odds ratio 
##   1.223502 
## 
## $c__Acidobacteriae
## odds ratio 
##   1.176164 
## 
## $c__Alphaproteobacteria
## odds ratio 
##   1.138922 
## 
## $c__Bacteroidia
## odds ratio 
##   1.557417 
## 
## $c__Gammaproteobacteria
## odds ratio 
##   1.233855 
## 
## $c__Gemmatimonadetes
## odds ratio 
##  0.7134832 
## 
## $c__Polyangia
## odds ratio 
##    3.37105 
## 
## $o__Burkholderiales
## odds ratio 
##  0.9959746 
## 
## $o__Caulobacterales
## odds ratio 
##   1.442557 
## 
## $o__Chitinophagales
## odds ratio 
##   1.366568 
## 
## $o__Cytophagales
## odds ratio 
##   1.876752 
## 
## $o__Flavobacteriales
## odds ratio 
##    3.37105 
## 
## $o__Gammaproteobacteria_Incertae_Sedis
## odds ratio 
##   4.534428 
## 
## $o__Gemmatimonadales
## odds ratio 
##  0.7134832 
## 
## $o__Rhizobiales
## odds ratio 
##   2.283077 
## 
## $o__Sphingomonadales
## odds ratio 
##  0.3304971 
## 
## $o__Xanthomonadales
## odds ratio 
##   1.718467 
## 
## $f__Caulobacteraceae
## odds ratio 
##    1.50201 
## 
## $f__Chitinophagaceae
## odds ratio 
##   1.400058 
## 
## $f__Comamonadaceae
## odds ratio 
##   1.602046 
## 
## $f__Devosiaceae
## odds ratio 
##   22.69165 
## 
## $f__Gemmatimonadaceae
## odds ratio 
##  0.7134832 
## 
## $f__Oxalobacteraceae
## odds ratio 
##   1.158663 
## 
## $f__Rhizobiaceae
## odds ratio 
##   2.542972 
## 
## $f__Rhodanobacteraceae
## odds ratio 
##   1.496563 
## 
## $f__Sphingomonadaceae
## odds ratio 
##  0.3304971 
## 
## $f__Unknown_Family
## odds ratio 
##   4.534428 
## 
## $f__Xanthobacteraceae
## odds ratio 
##   1.978932 
## 
## $f__Xanthomonadaceae
## odds ratio 
##   1.790011 
## 
## $g__Acidibacter
## odds ratio 
##   4.534428 
## 
## $`g__Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium`
## odds ratio 
##   2.826685 
## 
## $g__Gemmatimonas
## odds ratio 
##    1.35984 
## 
## $g__Lysobacter
## odds ratio 
##   2.229048 
## 
## $g__Massilia
## odds ratio 
##   1.247307 
## 
## $g__Niastella
## odds ratio 
##   2.805325

6.5 - calculate proportions of the fisher test

this functions will now take p values and oods ratio out of the fisher test output and into the heat tree

# extract p values and odds ration from the fisher tests and put it into the heat tree
fisher_to_heatTree(fisher_output = fisher_output_at, 
                   ps_important_taxa = imp_ASV_ps_l$At)

6.6 - make one fisher summary heat tree per species

now, run these custom functions over lists of phyloseq objects separated by species with mapply

# run the custom function voer 2 lists of philoseq objects, one with imporntat taxa and other with the full taxa (for every partition)
fisher_result_l<-mapply(function (list_1,list_2)
                   fisher_all_taxa_groups(ps_important_taxa = list_1, 
                                          ps_all_taxa = list_2),
                   list_1 = imp_ASV_ps_l,
                   list_2 = ps_l,
                   SIMPLIFY = FALSE)


#run the custom function on a list of 3 species
fisher_summaries_3l<-mapply(function(list_1,list_2)
    fisher_to_heatTree(fisher_output = list_1,
                       ps_important_taxa = list_2),
    list_1 = fisher_result_l,
    list_2 = imp_ASV_ps_l,
    SIMPLIFY = FALSE)

that’s it, thank you for your time!

I hope you found the workshop insightful and that you will find the code useful as a template you can improve. feel free to share this with collegues. as you know, you will need many microbiome tutorials for a complete, updated analysis.

BONUS FOR THE CURIOUS

Are you curious about all the data wrangling of these 2 custom functions? here you can execute a hard-coded version of the function, where you can visualize each object step by step.

#hard-code input arguments of fisher_all_taxa_groups()
ps_important_taxa<-imp_ASV_ps_l$At
fisher_output<-fisher_result



#######################################
######## make metacoder object ######## 
#######################################
imp_heat_ps<-ps_important_taxa

#remove unecessary taxonomic indo (dada2id, "S__" and" above_selected)
tax_table(imp_heat_ps)<-tax_table(imp_heat_ps)[,1:6]

# let's remove the "r__"ranks from the taxonomy, they can be useful but will pollute our plot
tax_table(imp_heat_ps)[, colnames(tax_table(imp_heat_ps))] <- gsub(pattern = "[a-z]__", # regular expression pattern to search for
                                                                   x = tax_table(imp_heat_ps)[, colnames(tax_table(imp_heat_ps))], # "df"
                                                                   replacement = "") # replacement for pattern

# transform from phyloseq to  taxmap object
imp_heat<-parse_phyloseq(imp_heat_ps)
## The following 22 of 114 (19.3%) input indexes have `NA` in their classifications:
##    8, 11, 12, 15, 17, 18, 19, 22 ... 66, 67, 90, 97, 98, 106, 110
#######################################
######## aplly fdr to fisher ######## 
#######################################

#apply FDR correction to the p values

  #single df with all p values
  pvector<-do.call(rbind.data.frame, map(fisher_output,1))
  
  # adjust the p value with fdr, then save as a list
  adjusted_p<-p.adjust(p = pvector[[1]],
                          method = "fdr")%>%
                      as.list()
  # adjust names
  names(adjusted_p)<-names(fisher_output)

  

#this is the name that mustch match our fisher p list
taxon_id_metacoder<-lapply(imp_heat$taxa, function (x)
                                                    x$get_name())%>%
                                                    map(1)


# now turn that list into a df
taxon_id_metacoder<-do.call(rbind.data.frame, map(taxon_id_metacoder,1))

# and change column name
colnames(taxon_id_metacoder)<-"taxa_id"

# truns the p values into a dataframe
adjusted_p_input<-as.data.frame(t(as.data.frame(adjusted_p)))%>%
  rownames_to_column()

#change column names
colnames(adjusted_p_input)<-c("taxa_id", "fdr_p")
  
# now join both df
fdr_for_heatTree<-left_join(taxon_id_metacoder,
                            adjusted_p_input,
                            by = "taxa_id")



#######################################################################
################### add fold changes! ########################################
#######################################################################


# get odds ratio for each taxa
odd_list<- map(fisher_output,3)%>%
  map(1)

# truns the p values into a dataframe
odd_list<-as.data.frame(t(as.data.frame(odd_list)))%>%
  rownames_to_column()

#change column names
colnames(odd_list)<-c("taxa_id", "odds_ratio")

# now join both df
fisher_for_heatTree<-left_join(fdr_for_heatTree,
                            odd_list,
                            by = "taxa_id")




#NAs will crash metadore. let's change them with 0.9999 for now
fisher_for_heatTree[is.na(fisher_for_heatTree)]<-"1"

#change structure to numeric
fisher_for_heatTree$fdr_p<-as.numeric(fisher_for_heatTree$fdr_p)
fisher_for_heatTree$odds_ratio<-as.numeric(fisher_for_heatTree$odds_ratio)


#now let's dim p values above 0.1, or else we can get counfounded
#fisher_for_heatTree$fdr_p[fisher_for_heatTree$fdr_p>0.1]<-1







#######################################################################
############### now plot the heat tree ############### 
#######################################################################



set.seed(1)
      output<- heat_tree(imp_heat,
                 node_size = fisher_for_heatTree$odds_ratio, # n_obs is a function that calculates, in this case, the number of OTUs per taxon
                 node_color = fisher_for_heatTree$fdr_p,
                 node_label = taxon_names,
                 node_size_axis_label = "Size: oods_ratio",
                 node_color_interval = c(0, 0.15),
                 edge_color_interval = c(0, 0.15),
                 node_color_range = c("darkgreen", "seagreen", "yellowgreen", "grey"),
                 edge_color_range = c("darkgreen", "seagreen", "yellowgreen", "grey"),
                 node_color_axis_label = "Color: FDR-adjusted p",
                 layout = "davidson-harel", # The primary layout algorithm
                 initial_layout = "reingold-tilford") # The layout algorithm that initializes node locations